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Dusp1 (NM_013642) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR205652
Dusp1 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 1 (Dusp1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dusp1
Synonyms 3CH134; erp; mkp-1; MKP1; Ptpn16; U19515
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205652 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGATGGAGGTGGGCATCCTGGACGCCGGGGGGCTGCGCGCGCTGCTGCGAGAGGGCGCCGCGCAGT
GCCTGTTGTTGGATTGTCGCTCCTTCTTCGCTTTCAACGCCGGCCACATCGCGGGCTCAGTGAACGTGCG
CTTCAGCACCATCGTGCGTCGCCGCGCCAAGGGCGCCATGGGCCTGGAGCATATCGTGCCCAACGCTGAA
CTGCGTGGCCGCCTGCTGGCCGGAGCCTACCACGCCGTGGTGCTGCTGGACGAGCGCAGCGCCTCCCTGG
ACGGCGCCAAGCGCGACGGCACCCTGGCCCTGGCCGCGGGCGCGCTCTGCCGAGAGGCGCGCTCCACTCA
AGTCTTCTTTCTCCAAGGAGGATATGAAGCGTTTTCGGCTTCCTGCCCTGAGCTGTGCAGCAAACAGTCC
ACCCCCACGGGGCTCAGCCTCCCCCTGAGTACTAGTGTGCCTGACAGTGCAGAATCCGGATGCAGCTCCT
GTAGTACCCCTCTCTACGATCAGGGGGGCCCAGTGGAGATCCTGTCCTTCCTGTACCTGGGCAGTGCCTA
TCACGCTTCTCGGAAGGATATGCTTGACGCCTTGGGCATCACCGCCTTGATCAACGTCTCAGCCAATTGT
CCTAACCACTTTGAGGGTCACTACCAGTACAAGAGCATCCCTGTGGAGGACAACCACAAGGCAGACATCA
GCTCCTGGTTCAACGAGGCTATTGACTTCATAGACTCCATCAAGGATGCTGGAGGGAGAGTGTTTGTTCA
TTGCCAGGCCGGCATCTCCCGGTCAGCCACCATCTGCCTTGCTTACCTCATGAGGACTAACCGGGTAAAG
CTGGACGAGGCCTTTGAGTTTGTGAAGCAGAGGCGGAGTATCATCTCCCCGAACTTCAGCTTCATGGGCC
AGCTGCTGCAGTTTGAGTCCCAAGTGCTAGCCCCTCACTGCTCTGCTGAAGCTGGGAGCCCTGCCATGGC
TGTCCTTGACCGGGGCACCTCTACTACCACAGTCTTCAACTTCCCTGTCTCCATCCCCGTCCACCCCACG
AACAGTGCCCTGAACTACCTTAAAAGCCCCATCACCACCTCTCCAAGCTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR205652 protein sequence
Red=Cloning site Green=Tags(s)

MVMEVGILDAGGLRALLREGAAQCLLLDCRSFFAFNAGHIAGSVNVRFSTIVRRRAKGAMGLEHIVPNAE
LRGRLLAGAYHAVVLLDERSASLDGAKRDGTLALAAGALCREARSTQVFFLQGGYEAFSASCPELCSKQS
TPTGLSLPLSTSVPDSAESGCSSCSTPLYDQGGPVEILSFLYLGSAYHASRKDMLDALGITALINVSANC
PNHFEGHYQYKSIPVEDNHKADISSWFNEAIDFIDSIKDAGGRVFVHCQAGISRSATICLAYLMRTNRVK
LDEAFEFVKQRRSIISPNFSFMGQLLQFESQVLAPHCSAEAGSPAMAVLDRGTSTTTVFNFPVSIPVHPT
NSALNYLKSPITTSPSC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013642
ORF Size 1101 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013642.1, NM_013642.2, NM_013642.3, NP_038670.1
RefSeq Size 1952 bp
RefSeq ORF 1104 bp
Locus ID 19252
UniProt ID P28563
Cytogenetics 17 13.28 cM
MW 39.4 kDa
Summary Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.UniProtKB/Swiss-Prot Function
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"NM_013642" in other vectors (4)
SKU Description Size Price
MC200497 Dusp1 (untagged) - Mouse dual specificity phosphatase 1 (Dusp1), (10ug) 10 ug
$457.00
MG205652 Dusp1 (tGFP-tagged) - Mouse dual specificity phosphatase 1 (Dusp1) 10 ug
$489.00 $657.00
MR205652L3 Lenti ORF clone of Dusp1 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 1 (Dusp1) 10 ug
$757.00
MR205652L4 Lenti ORF clone of Dusp1 (mGFP-tagged) - Mouse dual specificity phosphatase 1 (Dusp1) 10 ug
$757.00

Plasmid Map for MR205652

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR205652
Circular map for MR205652

file-alt Citations

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