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Nudt12 (BC057657) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR205643
Nudt12 (Myc-DDK-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 12 (cDNA clone MGC:68086 IMAGE:2646355)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nudt12
Synonyms 0610016O18Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205643 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTCTGTTAAAAGAAATCCCAAAAAGGAGATGATTTCTGAACTCCACAGTTCAGCCGCAGAGGGAA
ATGTCGCAAAGTTAGCAGGAATACTCAGCCATTCTCCATCTCTTCTCAATGAGACTTCTGAAAATGGATG
GACTGCTTTAATGTACGCCGCCAGGAACGGGCATCCCGATGTGGTCCAGTTTCTGCTTGAGAAAGGATGT
GACAGATCACTTGTCAATAAAGGGAGGCAAACCGCCCTGGATATTGCTGCATTTTGGGGTTATAGGCATA
TAGCTAACTTGTTAGCAAATGCAAAAGGTGGGAAGAAGCCCTGGTTCCTAACCAATGAAGTAGACGAGTG
TGAGAATTATTTTAGCAGAACACTACTGGACCGGAGGAGTGACAAAAGAAATAATTCTGACTGGCTGCAA
GCTAAAGAGAGCCACCCCACCACAGTTTATCTCCTTTTCTCAGACTTGAACCCCCTGGTTACCCTAGGTG
GTAATAAAGAAAGCTCGCAGCAGCCGGAAGTCCGGCTTTGCCAGCTGAACTACCCGGATGTAAAGGGTTA
CTTGGCTCAGCCTGAGAAGATCACCTTGGTGTTCCTTGGAGTCGAGCTTGAGATGAGGAAAGGCTCACCC
GCCCAGGCGGGAGGAGTCCCAGAGGAAGAGGAGGACGGTTTGGTTGCTTGGTTTGCCCTCGGTATTGAAC
CCGGTGCTGCTGAGGAGTTTAAGCAAAGACATGAAAATTGTTATTTTCTCCACCCGCCAATGCCAGCTCT
TCTGCAGTTGAAAGAAAAGGAGGCTGGGGTGGTAGCTCAAGCAAGATCAGTGCTTGCCTGGCATAGTCGA
TACAAGTTCTGCCCAACCTGTGGCAGTGTGACTAAAATTGAGGAAGGAGGCTACAAAAGAGTGTGTGTAC
GAGAGACTTGCCCTAGTCTCCAAGGCGTCCACAACACATCTTACCCGAGAGTTGATCCAGTCGTAATTAT
GCAGGTCATCCATCCAGATGGAACCAAATGTCTTCTAGGCAGGCAAAAGCGATTTCCCCCGGGCATGTTC
ACATGTCTTGCTGGATTTATAGAGCCCGGTAAGCCCATACTGACTGGTTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR205643 protein sequence
Red=Cloning site Green=Tags(s)

MSSVKRNPKKEMISELHSSAAEGNVAKLAGILSHSPSLLNETSENGWTALMYAARNGHPDVVQFLLEKGC
DRSLVNKGRQTALDIAAFWGYRHIANLLANAKGGKKPWFLTNEVDECENYFSRTLLDRRSDKRNNSDWLQ
AKESHPTTVYLLFSDLNPLVTLGGNKESSQQPEVRLCQLNYPDVKGYLAQPEKITLVFLGVELEMRKGSP
AQAGGVPEEEEDGLVAWFALGIEPGAAEEFKQRHENCYFLHPPMPALLQLKEKEAGVVAQARSVLAWHSR
YKFCPTCGSVTKIEEGGYKRVCVRETCPSLQGVHNTSYPRVDPVVIMQVIHPDGTKCLLGRQKRFPPGMF
TCLAGFIEPGKPILTGF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC057657
ORF Size 1101 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC057657, AAH57657
RefSeq Size 1299 bp
RefSeq ORF 1103 bp
Locus ID 67993
Cytogenetics 17 D
MW 40.8 kDa
Summary Hydrolyzes NAD(P)H to NMNH and AMP (2',5'-ADP), and diadenosine diphosphate to AMP. Has also activity towards NAD(P)(+), ADP-ribose and diadenosine triphosphate. May act to regulate the concentration of peroxisomal nicotinamide nucleotide cofactors required for oxidative metabolism in this organelle (By similarity).UniProtKB/Swiss-Prot Function
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"BC057657" in other vectors (4)
SKU Description Size Price
MC218395 Nudt12 (untagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 12 (cDNA clone MGC:68086 IMAGE:2646355), (10ug) 10 ug
$503.00
MG205643 Nudt12 (tGFP-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 12 (cDNA clone MGC:68086 IMAGE:2646355) 10 ug
$657.00
MR205643L3 Lenti ORF clone of Nudt12 (Myc-DDK-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 12 (cDNA clone MGC:68086 IMAGE:2646355) 10 ug
$757.00
MR205643L4 Lenti ORF clone of Nudt12 (mGFP-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 12 (cDNA clone MGC:68086 IMAGE:2646355) 10 ug
$757.00

Plasmid Map for MR205643

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR205643
Circular map for MR205643

file-alt Citations

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