Rtca (NM_025517) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205629
Rtca (Myc-DDK-tagged) - Mouse RNA terminal phosphate cyclase domain 1 (Rtcd1)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rtca
Synonyms 2310009A18Rik; AI450277; RPC; Rtcd1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205629 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGGCCAGCGGGTGGAGGTGGACGGTGGGATCATGGAAGGGGGCGGCCAGATCCTCAGGGTCTCCA
CCGCCCTGAGCTGCCTCCTGGGCCTACCCTTGCGCGTGCAGAAGATCCGCGCGGGCCGCAGCACGCCGGG
CCTCAGGCCTCAGCATTTATCTGGACTGGAAATGGTTCGTGATTTGTGTGGCGGGCACCTGGAGGGAGCA
GAAATCGGCTCAACAGAGATAACCTTTACCCCCGAGAAGATCAGAGGTGGAGTCCACACGGCAGATACCA
AGACAGCAGGGAGTGTGTGCCTCTTGATGCAGGTTTCAATGCCCTGTGTCCTGTTTGCGGCTTCCCCGTC
GGAGCTCCGTCTGAAAGGCGGCACCAATGCGGAGATGGCCCCGCAGATCGATTACACCATGATGGTTTTC
AAGCCAATTGCTGAGAAGTTTGGTTTCACATTTAATTGTGACATTAAAACGAGGGGCTATTACCCCAAAG
GGGGTGGTGAAGTGATTGTCCGAGTGTCCCCAGTTAAACGATTGGACCCAATAAATCTGACTGATCGAGG
CTCTGTGACCAAGATTTATGGGAGAGCTTTTGTTGCCGGTGTTTTGCCACTAAAAGTAGCAAAAGATATG
GCGGCGGCGGCTGTGCGGTGCATCCGAAAGGAGATCCGGGATCTGTACGTCAGCATCCAGCCCGTGCAGG
AGGCCAGGGACCAAGCCTTCGGCAATGGGAGTGGAATCATCATTGTTGCTGAGACATCCACTGGCTGCTT
GTTTGCTGGATCATCACTTGGCAAAAGAGGTGTGAATGCAGACAAGGTTGGGATTGAAGCTGCTGAAATG
CTATTAGCGAACCTTAGACACGGTGGCACTGTGGATGAGTATCTACAAGACCAGCTGATTATTTTCATGG
CGCTAGCCAATGGAATTTCCAGAATAAAAACAGGGCCAGTCACACTCCACACACAGACTGCTATACATTT
TGCTGAACAGCTAGCAAAGGCCAAATTTACCGTGAAGAAATCAGAAGAGGAAGAGGATGCCACTAAGGAC
ACTTACATCATCGAGTGTGAAGGAATTGGGATGGCAAATCCACACCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205629 protein sequence
Red=Cloning site Green=Tags(s)

MEGQRVEVDGGIMEGGGQILRVSTALSCLLGLPLRVQKIRAGRSTPGLRPQHLSGLEMVRDLCGGHLEGA
EIGSTEITFTPEKIRGGVHTADTKTAGSVCLLMQVSMPCVLFAASPSELRLKGGTNAEMAPQIDYTMMVF
KPIAEKFGFTFNCDIKTRGYYPKGGGEVIVRVSPVKRLDPINLTDRGSVTKIYGRAFVAGVLPLKVAKDM
AAAAVRCIRKEIRDLYVSIQPVQEARDQAFGNGSGIIIVAETSTGCLFAGSSLGKRGVNADKVGIEAAEM
LLANLRHGGTVDEYLQDQLIIFMALANGISRIKTGPVTLHTQTAIHFAEQLAKAKFTVKKSEEEEDATKD
TYIIECEGIGMANPHL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025517
ORF Size 1098 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025517.1, NM_025517.2, NM_025517.3, NP_079793.1
RefSeq Size 1576 bp
RefSeq ORF 1101 bp
Locus ID 66368
UniProt ID Q9D7H3
Cytogenetics 3 G1
MW 39.3 kDa
Summary Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025517" in other vectors (3)
SKU Description Size Price
MG205629 Rtca (tGFP-tagged) - Mouse RNA terminal phosphate cyclase domain 1 (Rtcd1) 10 ug
$657.00
MR205629L3 Lenti ORF clone of Rtca (Myc-DDK-tagged) - Mouse RNA terminal phosphate cyclase domain 1 (Rtcd1) 10 ug
$757.00
MR205629L4 Lenti ORF clone of Rtca (mGFP-tagged) - Mouse RNA terminal phosphate cyclase domain 1 (Rtcd1) 10 ug
$757.00

Plasmid Map for MR205629

Download sequence for

Cloning scheme for MR205629

Circular map for MR205629

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