Lrrc19 (NM_175305) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205609
Lrrc19 (Myc-DDK-tagged) - Mouse leucine rich repeat containing 19 (Lrrc19)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lrrc19
Synonyms 9130022A01Rik; AI314124; AL022954; AW261791
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205609 representing NM_175305
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGTCACACGCTTCATGTTTTGGCTCTTCTCCATGCTACTACCATCTGTCAAAAGCCAAGCTTCTG
AAACAGAAGTCCCATGTAATTTCAGCAGAAGGAATTACACTTTGATTCCAGAGGGTATCAGTACCAATGT
TACCATTCTTGATCTCAGTTATAACCGAATTACTCTGAATGCTGCAGATAGCAGGGTGCTGCAGATGTAC
TCTTTACTCACTGAGCTCTACCTGATGGAGAATAACATCATTGCCTTATATAACAGCAGTTTCAGGAATC
TTCTCAACCTAGAAATTTTAAATATCTGTGGAAATTCAATCAGTGTAATTCAACAGGGTTCATTTGTTGG
CTTAAATGAACTAAAACAATTATTTCTTTGCCAAAACAAAATATTACAACTGAATCCCGATACATTTGTG
CCTCTAAATAACCTAAAAGTTCTGAATCTGCAAGGCAATTTGATACGCCTCTTCGATGCACCACAGCTAC
CTCATCTGGAGATACTAACTCTGGATGGAAATCCATGGAACTGTACCTGTGGTCTACTTGAGTTGCACAA
CTGGCTGAACACGTCTAATGTGACATTAGAAAACGAGAACATGACCATGTGTAGCTACCCAGATGAGCTA
AAGCACGACAGCATCAAGTCAGCGCCCTTCACAACTGAATGCCACTCTACATTTATTTCCACTATAACTG
AAGACTTTCAGTCCACTAGGAATTCATCTTTCAATAGCTCTTCACACAACTTAACATGGACCTCAGAGCA
TGAGCCTCTTGGGAAAAGCTGGGCTTTCCTGGTTGGTGTTGTTGCCACTGTACTGCTAACTTCACTGCTT
ATCTTCATTGCTATCAAGTGCCCAGTATGGTACAATATTCTGCTGAGTTACAACCACCATCGCCTGGAAG
AGCATGAGGCAGAAACCTATGAAAATGGTCTTACAAGAAACCCAAGTTCTCTTTCACAAATAACAGATAC
GAACTCTGAAGACACCACAGTGATATTTGAACAGTTGCATGCATTTGTGGTAGATGATGATGGGTTTATT
GAAGACAGATACATAGATATCAATGAGGTACATGAAGAAAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205609 representing NM_175305
Red=Cloning site Green=Tags(s)

MKVTRFMFWLFSMLLPSVKSQASETEVPCNFSRRNYTLIPEGISTNVTILDLSYNRITLNAADSRVLQMY
SLLTELYLMENNIIALYNSSFRNLLNLEILNICGNSISVIQQGSFVGLNELKQLFLCQNKILQLNPDTFV
PLNNLKVLNLQGNLIRLFDAPQLPHLEILTLDGNPWNCTCGLLELHNWLNTSNVTLENENMTMCSYPDEL
KHDSIKSAPFTTECHSTFISTITEDFQSTRNSSFNSSSHNLTWTSEHEPLGKSWAFLVGVVATVLLTSLL
IFIAIKCPVWYNILLSYNHHRLEEHEAETYENGLTRNPSSLSQITDTNSEDTTVIFEQLHAFVVDDDGFI
EDRYIDINEVHEEK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175305
ORF Size 1092 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175305.5
RefSeq Size 2773 bp
RefSeq ORF 1095 bp
Locus ID 100061
UniProt ID Q8BZT5
Cytogenetics 4 C5
MW 42 kDa
Summary Pathogen-recognition receptor which mediates the activation of TRAF2- and TRAF6 NF-kappa-B signaling pathways and induces the expression of proinflammatory cytokines (PubMed:26776522, PubMed:19679103, PubMed:25026888). In kidney, prevents infection by uropathogenic bacteria by inducing the production of cytokines, chemokines and antimicrobial substances (PubMed:25026888). In gut, involved in host-microbiota interactions, plays a critical role in promoting the recruitment of immune cells and intestinal inflammation (PubMed:26776522).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175305" in other vectors (4)
SKU Description Size Price
MC212050 Lrrc19 (untagged) - Mouse leucine rich repeat containing 19 (Lrrc19), (10ug) 10 ug
$503.00
MG205609 Lrrc19 (tGFP-tagged) - Mouse leucine rich repeat containing 19 (Lrrc19) 10 ug
$657.00
MR205609L3 Lenti ORF clone of Lrrc19 (Myc-DDK-tagged) - Mouse leucine rich repeat containing 19 (Lrrc19) 10 ug
$757.00
MR205609L4 Lenti ORF clone of Lrrc19 (mGFP-tagged) - Mouse leucine rich repeat containing 19 (Lrrc19) 10 ug
$757.00

Plasmid Map for MR205609

Download sequence for

Cloning scheme for MR205609

Circular map for MR205609

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