OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Tmem30a (NM_133718) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR205608
Tmem30a (Myc-DDK-tagged) - Mouse transmembrane protein 30A (Tmem30a)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Tmem30a or NM_133718"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem30a
Synonyms 2010200I23Rik; AW540225; Cdc50a; D9Wsu20e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205608 representing NM_133718
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATT
CGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC

ATGGCGATGAACTATAGCGCGAAGGATGAGGTGGACGGCGGGCCCGCGGGTCCTCCCGGG
GGCGCCGCCAAGACCCGGAGGCCGGATAACACGGCCTTCAAACAGCAACGGCTACCCGCC
TGGCAGCCCATCCTCACGGCCGGCACGGTGCTGCCCACCTTCTTCATCATCGGCCTCATC
TTCATCCCCATCGGCATCGGCATCTTCGTCACCTCCAACAACATCCGTGAGATCGAGATT
GATTACACTGGAACAGAACCTTCCAGCCCCTGCAATAAATGTTTATCTCCGAATGTGACA
TCTTGTGCTTGTACCATTAACTTCACACTGAAACAGTCATTTGAGGGCAATGTGTTTATG
TATTATGGACTGTCTAATTTCTATCAAAATCATCGTCGCTACGTGAAATCTCGAGATGAT
AGCCAGTTAAATGGAGACCCTAGTGCTTTGCTTAATCCAAGTAAGGAATGTGAACCTTAT
AGAAGAAATGAAGACAGACCAATTGCGCCATGTGGCGCTATTGCCAACAGCATGTTTAAT
GATACGTTAGAATTGTATCTGGTTGCCAATGAATCTGATCCCAAGCCTATTCCAATTCCT
TTGAAGAAAAAAGGTATTGCTTGGTGGACAGATAAAAATGTGAAATTCAGAAATCCACCT
GGAAAAGAGAGCCTCGAAGAAAAGTTTAAAGATACAATAAAGCCAGTAAACTGGCATAAG
GCAGTATATGAGCTAGACCCTGAAGATGAAAGTAATAATGGATTCATAAATGAAGACTTT
ATAGTTTGGATGCGTACTGCAGCATTACCTACTTTTCGTAAGTTGTATCGTCTCATAGAG
CGGAGAGATGATTTACACCCAACATTACCAGCTGGACAGTACTTTTTGAACATCACATAC
AATTACCCTGTGCATTCCTTTGATGGACGGAAACGGATGATCTTGAGCACTATTTCATGG
ATGGGAGGAAAGAATCCATTTTTGGGAATTGCTTACATCACTATTGGATCCATCTCTTTC
CTTCTGGGAGTTGTACTGCTAGTAATTAATCATAAATATAGAAACAGTAGTAACACTGCT
GACATCACCATT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGAT
GACGACGATAAGGTTTAAA
Protein Sequence
>MR205608 protein sequence
Blue=ORF Red=Cloning site Green=Tag(s)

MAMNYSAKDEVDGGPAGPPGGAAKTRRPDNTAFKQQRLPAWQPILTAGTVLPTFFIIGLI
FIPIGIGIFVTSNNIREIEIDYTGTEPSSPCNKCLSPNVTSCACTINFTLKQSFEGNVFM
YYGLSNFYQNHRRYVKSRDDSQLNGDPSALLNPSKECEPYRRNEDRPIAPCGAIANSMFN
DTLELYLVANESDPKPIPIPLKKKGIAWWTDKNVKFRNPPGKESLEEKFKDTIKPVNWHK
AVYELDPEDESNNGFINEDFIVWMRTAALPTFRKLYRLIERRDDLHPTLPAGQYFLNITY
NYPVHSFDGRKRMILSTISWMGGKNPFLGIAYITIGSISFLLGVVLLVINHKYRNSSNTA
DITI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133718
ORF Size 1092 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133718.4
RefSeq Size 3680 bp
RefSeq ORF 1095 bp
Locus ID 69981
UniProt ID Q8VEK0
Cytogenetics 9 43.82 cM
MW 41.1 kDa
Summary Accessory component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. The beta subunit may assist in binding of the phospholipid substrate. Required for the proper folding, assembly and ER to Golgi exit of the ATP8A2:TMEM30A flippase complex. ATP8A2:TMEM30A may be involved in regulation of neurite outgrowth, and, reconstituted to liposomes, predomiminantly transports phosphatidylserine (PS) and to a lesser extent phosphatidylethanolamine (PE). The ATP8A1:TMEM30A flippase complex seems to play a role in regulation of cell migration probably involving flippase-mediated translocation of phosphatidylethanolamine (PE) at the plasma membrane. Required for the formation of the ATP8A2, ATP8B1 and ATP8B2 P-type ATPAse intermediate phosphoenzymes. Involved in uptake of platelet-activating factor (PAF). Can also mediate the export of alpha subunits ATP8A1, ATP8B1, ATP8B2, ATP8B4, ATP10A, ATP10B, ATP10D, ATP11A, ATP11B and ATP11C from the ER to other membrane localizations.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_133718" in other vectors (4)
SKU Description Size Price
MC200840 Tmem30a (untagged) - Mouse transmembrane protein 30A (Tmem30a), (10ug) 10 ug
$457.00
MG205608 Tmem30a (tGFP-tagged) - Mouse transmembrane protein 30A (Tmem30a) 10 ug
$657.00
MR205608L3 Lenti ORF clone of Tmem30a (Myc-DDK-tagged) - Mouse transmembrane protein 30A (Tmem30a) 10 ug
$757.00
MR205608L4 Lenti ORF clone of Tmem30a (mGFP-tagged) - Mouse transmembrane protein 30A (Tmem30a) 10 ug
$757.00

Plasmid Map for MR205608

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR205608
Circular map for MR205608

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products