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Tmem30a (NM_133718) Mouse Untagged Clone

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SKU
MC200840
Tmem30a (untagged) - Mouse transmembrane protein 30A (Tmem30a), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $457.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Tmem30a
Synonyms 2010200I23Rik; AW540225; Cdc50a; D9Wsu20e
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC018367 sequence for NM_133718
CGGACGCGTGGGCGGCACGCAAGTCCCCTGGGCGGCCCAGCTGGACGTGGCCGAGCCGAAGCCCGGGGGC CGATGGCGATGAACTATAGCGCGAAGGATGAGGTGGACGGCGGGCCCGCGGGTCCTCCCGGGGGCGCCGC CAAGACCCGGAGGCCGGATAACACGGCCTTCAAACAGCAACGGCTACCCGCCTGGCAGCCCATCCTCACG GCCGGCACGGTGCTGCCCACCTTCTTCATCATCGGCCTCATCTTCATCCCCATCGGCATCGGCATCTTCG TCACCTCCAACAACATCCGTGAGATCGAGATTGATTACACTGGAACAGAACCTTCCAGCCCCTGCAATAA ATGTTTATCTCCGAATGTGACATCTTGTGCTTGTACCATTAACTTCACACTGAAACAGTCATTTGAGGGC AATGTGTTTATGTATTATGGACTGTCTAATTTCTATCAAAATCATCGTCGCTACGTGAAATCTCGAGATG ATAGCCAGTTAAATGGAGACCCTAGTGCTTTGCTTAATCCAAGTAAGGAATGTGAACCTTATAGAAGAAA TGAAGACAGACCAATTGCGCCATGTGGCGCTATTGCCAACAGCATGTTTAATGATACGTTAGAATTGTAT CTGGTTGCCAATGAATCTGATCCCAAGCCTATTCCAATTCCTTTGAAGAAAAAAGGTATTGCTTGGTGGA CAGATAAAAATGTGAAATTCAGAAATCCACCTGGAAAAGAGAGCCTCGAAGAAAAGTTTAAAGATACAAT AAAGCCAGTAAACTGGCATAAGGCAGTATATGAGCTAGACCCTGAAGATGAAAGTAATAATGGATTCATA AATGAAGACTTTATAGTTTGGATGCGTACTGCAGCATTACCTACTTTTCGTAAGTTGTATCGTCTCATAG AGCGGAGAGATGATTTACACCCAACATTACCAGCTGGACAGTACTTTTTGAACATCACATACAATTACCC TGTGCATTCCTTTGATGGACGGAAACGGATGATCTTGAGCACTATTTCATGGATGGGAGGAAAGAATCCA TTTTTGGGAATTGCTTACATCACTATTGGATCCATCTCTTTCCTTCTGGGAGTTGTACTGCTAGTAATTA ATCATAAATATAGAAACAGTAGTAACACTGCTGACATCACCATTTAATTTTATATTCTGAAACCAAATCT ACTGCATGTGCATCAAGGCCAGTCCTGTTCAACCTAGCTTTTGAATGCTGATGTCTGGTTAGTATGTCAT TTTGAAGTTGGCACATAACTTTAAAAAAACAAAACAAACAAAACAGCCTTTGTTCTTTGCTTCTTACATA TGGATGACTTATGAAAATATATGATGGATATAAAAATTAGCCATATTGATTATATCAATATTGTAACTGC TAAAATGACATTCTAATGTCTGCTTTTTATTGGGACAGGCCATGTGATGCATAGAGCCTCTTTCCTATGA AATGCGTCTACTGCTTAAACTGTTGATGCTGTGTTGATAACATATTGACATGATGCTGTATATGTGTGCC TACTGTGTGATGAAAGGGATTATGAGATGTATGAGTGTAATGACTTGCTAACCTTTGAAAATTTGGTTAC AGTTCAGATAGAAGAAAGACTATAAATAAAACACTTCATCATTTTCATGTGTCGTGTGTAAAGGCTTAAA GTCCCTCCTTGTTGAGGTGGTTCATATGTTCAGTTGCTCTATTATGATTCTCCGATAATGACGTTGACTT CACACTTTAGCTTGTACAACATAGAAATTAATATCTAAAGAGGTCAGTGGGTCTTGGCTAGAATTTTAAA TTTCTTCTCATTTGAGTAAAATGTTGCATTCTGAAGTCCCATGCTACCTGAAGTTGCATTTGGAGTCCCA AGCTACTGGAATGTTTATATGTGACCGTTTCCCAGGAGGCTTACACTGCAGAAGGAAGAATGAATCTAGG TGAGGTGGGCAGCTGCTTGGCAGTCCTCTCTTGTGCCCCAACTGTAAACCAGATAGAAATGTTCAGGGGA GGATACTTTCATTATTGTGGTTTGTAGTGTTAAGATGATTGCTTCTGCCTTGGAAATACCTCAAGCTGTT CTTATTTAACAGGTAAGTGACTGAGTATAATATTCCAGAAAAATTTGAAATCCTAATTTCTTCCATATTT CATTAAATTTTTTTGCATACAGGTCTAACAAATATGGATATGTATACACATCCTCTTTAATGAAGGTATT ATTTTGGTTACTTTTCCTAAGATATACCTTAAAAGATGTTCTATACATTTCCTACTTAAATTCTGGGGGA TTTGGAGTATGTACATGATAAAAAAGATTATAATATATCGATTGAAGTTACTTTATTTTCTAATTAGAAT TATTTTAATAGTCCTTTATTGAATAAGTGCTGTAATTTGTTTGCTATGAGACTTATTCCTGATGTGAATG TAAATTATTTTTCCACATGCATGAAAAAATGTATGTACTAATCAGAGTTGTCTCCATTGCATTGAAATTA CTTGTTTTGAACTAAAGTAACTCATATTTATGTAGTAGAATGCTTATGTTTTCAGACTTTGTAATGATTT CCTTTGGATGTATTTTAAATCAATCGGTCTGGGTAACATATCAGTTTAGATTAATATGTGCTTAAAAGAA GAAAAAAATTCAATGGTTCATAGTAGAAATGTGCCACACTTAAATAAGCTCTGTATGACATGAAATTCTG TTAAAACATTGTAATTCATGGTGACTTTTAACTTATAAAAATACTACTTGCACGGGTTACTTGATTTATG GATATATGAAAACTTCTCAGGACGAAAGTTCTTCTTTCTCTAGAACTATTCTTCTGTCGGTCATGCAGAA TGCTGTTATTCTGAAAAGTGTCCCTGTTGCATATGATGGTCACTTTATTTGGGGGGATTCTTCATAAGAT GTGAGATGTTGATGCCAGTCTTTCCCAAGTAAGTGCTCGTAAAAAAGGACTACTAACTAGCCTGCATCTG TCTCTAACTGGGACCAAGGGGTCTGCTGAAGGAAACTGAAGAGCTCTAACATTTTCACAGCTTGGAGAAG ATAGAATCTTTAAAAGTACAACTGAAGCTTGATCTATTTTACAAGTGCATTGATGGCCCCTGTCCTTTTT TGGTTCCTGTCATTTGAAACCAACTCCTGTTGTAAATAGGAAGAATATGGGACATTCATATTTAAGAAAA TTTGATGTCATTAGGGGACTAAGTAGAAGGGTTAGAAAAAAGTATTCATTTGCAAGTATTTTGGCCCAAG AAATTTTCCAACTGAATAGTAAGCAAAAGGTAAGTTGTTTCATTGAAATCATAAGGCAGTTTAAGATAAA CTGGGGAAGATAACTGTTTTAATAGAGGATAATCGAATTGATTGTCAAGTGGATGTTATTTATTGGATAG GGACAGAGTTTATTTGTAACCTTAATTATATTAAAAGTTATTCTGTTAGGATGTTTTGTATTAATAAACG TGAACAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_133718
Insert Size 1095 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC018367, AAH18367
RefSeq Size 3555 bp
RefSeq ORF 1095 bp
Locus ID 69981
UniProt ID Q8VEK0
Cytogenetics 9 43.82 cM
Summary Accessory component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. The beta subunit may assist in binding of the phospholipid substrate. Required for the proper folding, assembly and ER to Golgi exit of the ATP8A2:TMEM30A flippase complex. ATP8A2:TMEM30A may be involved in regulation of neurite outgrowth, and, reconstituted to liposomes, predomiminantly transports phosphatidylserine (PS) and to a lesser extent phosphatidylethanolamine (PE). The ATP8A1:TMEM30A flippase complex seems to play a role in regulation of cell migration probably involving flippase-mediated translocation of phosphatidylethanolamine (PE) at the plasma membrane. Required for the formation of the ATP8A2, ATP8B1 and ATP8B2 P-type ATPAse intermediate phosphoenzymes. Involved in uptake of platelet-activating factor (PAF). Can also mediate the export of alpha subunits ATP8A1, ATP8B1, ATP8B2, ATP8B4, ATP10A, ATP10B, ATP10D, ATP11A, ATP11B and ATP11C from the ER to other membrane localizations.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_133718" in other vectors (4)
SKU Description Size Price
MG205608 Tmem30a (tGFP-tagged) - Mouse transmembrane protein 30A (Tmem30a) 10 ug
$657.00
MR205608 Tmem30a (Myc-DDK-tagged) - Mouse transmembrane protein 30A (Tmem30a) 10 ug
$289.00 $457.00
MR205608L3 Lenti ORF clone of Tmem30a (Myc-DDK-tagged) - Mouse transmembrane protein 30A (Tmem30a) 10 ug
$757.00
MR205608L4 Lenti ORF clone of Tmem30a (mGFP-tagged) - Mouse transmembrane protein 30A (Tmem30a) 10 ug
$757.00

file-alt Citations

Identification and functional analyses of disease-associated P4-ATPase phospholipid flippase variants in red blood cells
Liou, Molday, Wang et al
J. Biol. Chem. (2019) 294 (17), 6809-6821 DOI: 10.1074/jbc.RA118.007270
AI Summary: In this study, an OriGene mouse CDC50A clone (#MC200840) was used to investigate the functional roles of disease-associated P4-ATPase flippase variants in red blood cells.
Snippet: The ATP11C dephosphorylation-deficient mutant E181Q, disease associated and related mutants I355K, I355A, I355V, T415N, T415S, T415A, V357M and V357I were generated by site-directed mutagenesis. A clone of mouse CDC50A was obtained from OriGene (#MC200840). The accession numbers for mouse ATP11C DNA and Protein were NM_001037863.2 and Q9QZW0, respectively
Showing 1-1 of 1 papers.
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