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Zdhhc9 (BC042618) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR205587
Zdhhc9 (Myc-DDK-tagged) - Mouse zinc finger, DHHC domain containing 9 (cDNA clone MGC:36333 IMAGE:4950217)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Zdhhc9
Synonyms 6430508G22; 9530098M12Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205587 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGTGATGGTGGTAAGAAAGAAGGTGACACGGAAATGGGAGAAACTCCCAGGCAGGAACACCTTCT
GCTGTGATGGCCGCGTCATGATGGCCCGGCAAAAGGGCATCTTCTACCTGACCCTCTTCCTCATCCTGGG
GACATGTACACTCTTCTTTGCCTTCGAGTGTCGCTACCTGGCTGTTCAGCTGTCTCCTGCCATTCCTGTG
TTTGCCGCTATGCTCTTTCTTTTCTCCATGGCCACGCTGTTGAGGACAAGTTTCAGTGACCCTGGAGTGA
TTCCTCGAGCACTACCAGATGAAGCAGCTTTCATAGAAATGGAAATAGAAGCCACCAATGGTGCCGTGCC
ACAGGGCCAGCGACCACCTCCTCGTATTAAGAATTTCCAGATAAACAACCAGATTGTGAAACTGAAATAC
TGTTATACATGCAAGATCTTCCGGCCTCCCAGGGCCTCCCATTGTAGCATCTGTGACAACTGTGTGGAGC
GCTTCGACCATCACTGCCCCTGGGTGGGAAACTGTGTTGGAAAGAGGAACTACCGCTACTTCTACCTCTT
CATCCTCTCTCTCTCCCTCCTCACAATTTATGTCTTCGCCTTTAACATCGTCTATGTGGCCCTCAAATCC
TTGAAAATTGGCTTCCTGGAGACACTGAAAGAAACGCCTGGAACTGTTCTGGAAGTACTCATTTGCTTCT
TCACTCTCTGGTCTGTTGTGGGGCTGACTGGATTTCACACATTCCTTGTGGCTCTCAATCAGACCACCAA
TGAAGACATCAAAGGATCATGGACAGGGAAGAATCGTGTGCAGAATCCGTACAGCCACGGCAACATTGTG
AAGAACTGCTGTGAAGTGCTTTGTGGCCCCTTGCCCCCCAGTGTCCTGGATCGAAGGGGTATTTTGCCAC
TGGAGGAAAGTGGAAGTCGACCTCCAAGTACTCAAGAGACCAGCAGTAGCCTGTTGCCACAGAGCCCAGC
CTCCACAGAGCATATGAACTCTAATGAGATGGCAGAGGACACCAGCATTCCTGAAGAGATGCCACCTCCA
GAACCCCCAGAGCCACCACAGGAGGCATCCGAAGCTGAGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR205587 protein sequence
Red=Cloning site Green=Tags(s)

MSVMVVRKKVTRKWEKLPGRNTFCCDGRVMMARQKGIFYLTLFLILGTCTLFFAFECRYLAVQLSPAIPV
FAAMLFLFSMATLLRTSFSDPGVIPRALPDEAAFIEMEIEATNGAVPQGQRPPPRIKNFQINNQIVKLKY
CYTCKIFRPPRASHCSICDNCVERFDHHCPWVGNCVGKRNYRYFYLFILSLSLLTIYVFAFNIVYVALKS
LKIGFLETLKETPGTVLEVLICFFTLWSVVGLTGFHTFLVALNQTTNEDIKGSWTGKNRVQNPYSHGNIV
KNCCEVLCGPLPPSVLDRRGILPLEESGSRPPSTQETSSSLLPQSPASTEHMNSNEMAEDTSIPEEMPPP
EPPEPPQEASEAEK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC042618
ORF Size 1092 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC042618, AAH42618
RefSeq Size 2892 bp
RefSeq ORF 1094 bp
Locus ID 208884
Cytogenetics X A5
MW 40.9 kDa
Summary The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.UniProtKB/Swiss-Prot Function
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"BC042618" in other vectors (4)
SKU Description Size Price
MC206388 Zdhhc9 (untagged) - Mouse zinc finger, DHHC domain containing 9 (cDNA clone MGC:36333 IMAGE:4950217), (10ug) 10 ug
$457.00
MG205587 Zdhhc9 (tGFP-tagged) - Mouse zinc finger, DHHC domain containing 9 (cDNA clone MGC:36333 IMAGE:4950217) 10 ug
$657.00
MR205587L3 Lenti ORF clone of Zdhhc9 (Myc-DDK-tagged) - Mouse zinc finger, DHHC domain containing 9 (cDNA clone MGC:36333 IMAGE:4950217) 10 ug
$757.00
MR205587L4 Lenti ORF clone of Zdhhc9 (mGFP-tagged) - Mouse zinc finger, DHHC domain containing 9 (cDNA clone MGC:36333 IMAGE:4950217) 10 ug
$757.00

Plasmid Map for MR205587

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR205587
Circular map for MR205587

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