Vsx2 (NM_007701) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205529
Vsx2 (Myc-DDK-tagged) - Mouse visual system homeobox 2 (Vsx2)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Vsx2
Synonyms Chx10; Hox-1; Hox-10; Hox1; Hox10; or
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205529 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGGGAAAGCGGGGGAAGCGCTGAGCAAGCCCAAATCCGAGACAGTGGCCAAGAGTACCTCGGGGG
GCGCCCCGGCCAGGTGCACAGGGTTCGGCATCCAGGAGATCCTAGGCTTGAACAAGGAGCCTCCCAGCTC
CCACCCGCGGGCTGCCCTCGACGGCCTGGCCCCTGGGCATTTGTTAGCTGCGCGCTCCGTTCTCAGCCCG
GCAGGAGTGGGCAGCATGGGGCTGCTGGGGCCCGGGGGACTTCCCGGCTTCTACACACAGCCCACCTTCT
TGGAAGTGCTGTCCGATCCGCAGAGCGTCCACTTGCAGCCACTAGGCAGAGCGTCGGGGCCGCTGGACAC
CAGCCAGACGGCCAGCTCCGATTCCGAAGATGTTTCCTCCAGTGACCGAAAAATGTCCAAATCGGCTTTA
AACCAGACCAAGAAGCGTAAGAAGCGGCGACACAGGACAATCTTTACTTCCTACCAGCTAGAGGAGCTGG
AGAAAGCATTCAATGAAGCCCACTACCCAGATGTCTACGCCCGGGAGATGCTGGCCATGAAAACGGAGCT
CCCAGAAGACAGGATACAGGTGTGGTTCCAGAACCGCAGAGCCAAGTGGAGGAAGAGGGAGAAGTGCTGG
GGCCGGAGCAGCGTCATGGCTGAGTATGGTCTCTATGGAGCCATGGTGAGGCACTCGATCCCCCTGCCAG
AGTCTATCCTCAAGTCTGCCAAGGATGGCATAATGGATTCCTGTGCCCCATGGCTACTGGGGATGCACAA
AAAGTCGCTGGAGGCAGCAGCTGAGTCGGGAAGGAAGCCCGAGGTGGAACGCCAGGCCCTGCCCAAGCTC
GACAAGATGGAGCAGGAGGAACGGGCCCCAGAGGCCCAGGCAGCCATCTCCCAGGAAGAACTGAGGGAGA
ACAGCATTGCCGCCCTCCGAGCCAAAGCTCAGGAACACAGCACCAAGGTTCTGGGGACTGTATCTGGGCC
TGACAGCCTGGCCAGAAATGCTGAGAAGCCAGAAGAAGAGGACGCCACGGAGGAAGACAGGCCAGCTGAG
AAGCTCAGCCCACCGCAGCTGGAGGACATGGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205529 protein sequence
Red=Cloning site Green=Tags(s)

MTGKAGEALSKPKSETVAKSTSGGAPARCTGFGIQEILGLNKEPPSSHPRAALDGLAPGHLLAARSVLSP
AGVGSMGLLGPGGLPGFYTQPTFLEVLSDPQSVHLQPLGRASGPLDTSQTASSDSEDVSSSDRKMSKSAL
NQTKKRKKRRHRTIFTSYQLEELEKAFNEAHYPDVYAREMLAMKTELPEDRIQVWFQNRRAKWRKREKCW
GRSSVMAEYGLYGAMVRHSIPLPESILKSAKDGIMDSCAPWLLGMHKKSLEAAAESGRKPEVERQALPKL
DKMEQEERAPEAQAAISQEELRENSIAALRAKAQEHSTKVLGTVSGPDSLARNAEKPEEEDATEEDRPAE
KLSPPQLEDMA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007701
ORF Size 1083 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007701.3
RefSeq Size 3234 bp
RefSeq ORF 1086 bp
Locus ID 12677
UniProt ID Q61412
Cytogenetics 12 39.28 cM
MW 39.4 kDa
Summary This gene encodes a member of the Vsx (visual system homeobox) family which belongs to the larger PRD homeobox class. The encoded protein is required for eye organogenesis and controls retinal development. Disruption of this gene is associated with ocular retardation J (orJ), a mouse disease which causes microphthalmia, retinal degeneration and optic nerve aplasia. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2014
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Documents
Resources
"NM_007701" in other vectors (4)
SKU Description Size Price
MC205428 Vsx2 (untagged) - Mouse visual system homeobox 2 (Vsx2), (10ug) 10 ug
$457.00
MG205529 Vsx2 (tGFP-tagged) - Mouse C. elegans ceh-10 homeo domain containing homolog (Chx10) 10 ug
$657.00
MR205529L3 Lenti ORF clone of Vsx2 (Myc-DDK-tagged) - Mouse visual system homeobox 2 (Vsx2) 10 ug
$757.00
MR205529L4 Lenti ORF clone of Vsx2 (mGFP-tagged) - Mouse visual system homeobox 2 (Vsx2) 10 ug
$757.00

Plasmid Map for MR205529

Download sequence for

Cloning scheme for MR205529

Circular map for MR205529

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