Tpte2 (BC016580) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205381
Tpte2 (Myc-DDK-tagged) - Mouse transmembrane phosphoinositide 3-phosphatase and tensin homolog 2 (cDNA clone MGC:27763 IMAGE:2655857)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tpte2
Synonyms CRLM2; Ly114; Tpte2; Tslpr
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205381 representing BC016580
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCTTCTGGAACGCGCTGACGTCACGGGCTGCTCCCCTGATCCCCGCCCTGCCCCCGCAGGTGATG
TCACAGTCGTCTGCCATGACCTGGAGACGGTGGAGGTCACGTGGGGCTCGGGCCCCGACCACCACAGCGC
CAACTTGAGCCTGGAGTTCCGTTATGGTACTGGCGCCCTGCAACCCTGCCCGCGATATTTCCTGTCCGGC
GCTGGTGTCACTTCCGGGTGCATCCTCCCCGCGGCGAGGGCGGGGCTGCTGGAGCTGGCACTGCGCGACG
GAGGCGGGGCCATGGTGTTTAAGGCTAGGCAGCGCGCGTCCGCCTGGCTGAAGCCCCGCCCACCTTGGAA
TGTGACGCTGCTCTGGACACCAGACGGGGACGTGACTGTCTCCTGGCCTGCCCACTCCTACCTGGGCCTG
GACTACGAGGTGCAGCACCGGGAGAGCAATGACGATGAGGACGCCTGGCAGACGACCTCAGGGCCCTGCT
GTGACTTGACAGTGGGCGGGCTCGACCCCGCGCGCTGCTATGACTTCCGGGTTCGGGCGTCGCCCCGGGC
CGCGCACTATGGCCTGGAGGCGCAGCCTAGCGAGTGGACAGCGGTGACAAGGCTTTCCGGGGCAGCATCC
GCGGCCTCCTGTACCGCAAGCCCCGCCCCATCCCCGGCCCTGGCCCCGCCCCTCCTGCCCCTGGGCTGCG
GCCTAGCAGCGCTGCTGACACTGTCCCTGCTCCTGGCCGCCCTGAGGCTTCGCAGGGTGAAAGATGCGCT
GCTGCCCTGCGTCCCTGACCCCAGCGGCTCCTTCCCTGGACTCTTTGAGAAGCATCACGGGAACTTCCAG
GCCTGGATTGCGGACGCCCAGGCCACAGCCCCGCCAGCCAGGACCGAGGAGGAAGATGACCTCATCCACC
CCAAGGCTAAGAGGGTGGAGCCCGAGGATGGCACCTCCCTCTGCACCGTGCCAAGGCCACCCAGCTTCGA
GCCAAGGGGGCCGGGAGGCGGGGCCATGGTGTCAGTGGGCGGGGCCACGTTCATGGTGGGCGACAGCGGC
TACATGACCCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205381 representing BC016580
Red=Cloning site Green=Tags(s)

MALLERADVTGCSPDPRPAPAGDVTVVCHDLETVEVTWGSGPDHHSANLSLEFRYGTGALQPCPRYFLSG
AGVTSGCILPAARAGLLELALRDGGGAMVFKARQRASAWLKPRPPWNVTLLWTPDGDVTVSWPAHSYLGL
DYEVQHRESNDDEDAWQTTSGPCCDLTVGGLDPARCYDFRVRASPRAAHYGLEAQPSEWTAVTRLSGAAS
AASCTASPAPSPALAPPLLPLGCGLAALLTLSLLLAALRLRRVKDALLPCVPDPSGSFPGLFEKHHGNFQ
AWIADAQATAPPARTEEEDDLIHPKAKRVEPEDGTSLCTVPRPPSFEPRGPGGGAMVSVGGATFMVGDSG
YMTL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC016580
ORF Size 1062 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC016580.1
RefSeq Size 1425 bp
RefSeq ORF 1064 bp
Locus ID 57914
Cytogenetics 5 F
MW 52.3 kDa
Summary Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2. Implicated in the development of the hematopoietic system.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC016580" in other vectors (3)
SKU Description Size Price
MG205381 Tpte2 (tGFP-tagged) - Mouse transmembrane phosphoinositide 3-phosphatase and tensin homolog 2 (cDNA clone MGC:27763 IMAGE:2655857) 10 ug
$657.00
MR205381L3 Lenti ORF clone of Tpte2 (Myc-DDK-tagged) - Mouse transmembrane phosphoinositide 3-phosphatase and tensin homolog 2 (cDNA clone MGC:27763 IMAGE:2655857) 10 ug
$757.00
MR205381L4 Lenti ORF clone of Tpte2 (mGFP-tagged) - Mouse transmembrane phosphoinositide 3-phosphatase and tensin homolog 2 (cDNA clone MGC:27763 IMAGE:2655857) 10 ug
$757.00

Plasmid Map for MR205381

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Cloning scheme for MR205381

Circular map for MR205381

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