Mul1 (NM_026689) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR205346
Mul1 (Myc-DDK-tagged) - Mouse mitochondrial ubiquitin ligase activator of NFKB 1 (Mul1), nuclear gene encoding mitochondrial protein
$289.00
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Mul1 |
| Synonyms | 0610009K11Rik; AV000801; Gide |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_026689 |
| ORF Size | 1056 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_026689.1, NM_026689.2, NM_026689.3, NP_080965.2 |
| RefSeq Size | 3732 bp |
| RefSeq ORF | 1059 bp |
| Locus ID | 68350 |
| UniProt ID | Q8VCM5 |
| Cytogenetics | 4 D3 |
| MW | 39.8 kDa |
| Summary | Exhibits weak E3 ubiquitin-protein ligase activity (By similarity). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates (By similarity). Can ubiquitinate AKT1 preferentially at 'Lys-284' involving 'Lys-48'-linked polyubiquitination and seems to be involved in regulation of Akt signaling by targeting phosphorylated Akt to proteosomal degradation (By similarity). Proposed to preferentially act as a SUMO E3 ligase at physiological concentrations (By similarity). Plays a role in the control of mitochondrial morphology by promoting mitochondrial fragmentation, and influences mitochondrial localization (By similarity). Likely to promote mitochondrial fission through negatively regulating the mitochondrial fusion proteins MFN1 and MFN2, acting in a pathway that is parallel to the PRKN/PINK1 regulatory pathway (PubMed:24898855). May also be involved in the sumoylation of the membrane fission protein DNM1L (By similarity). Inhibits cell growth (By similarity). When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis (By similarity). Involved in the modulation of innate immune defense against viruses by inhibiting DDX58-dependent antiviral response (By similarity). Can mediate DDX58 sumoylation and disrupt its polyubiquitination (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_026689" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC207664 | Mul1 (untagged) - Mouse mitochondrial ubiquitin ligase activator of NFKB 1 (Mul1), nuclear gene encoding mitochondrial protein, (10ug) | 10 ug |
$503.00
|
|
| MG205346 | Mul1 (tGFP-tagged) - Mouse RIKEN cDNA 0610009K11 gene (0610009K11Rik) | 10 ug |
$489.00
$657.00
|
|
| MR205346L1 | Lenti ORF clone of Mul1 (Myc-DDK-tagged) - Mouse mitochondrial ubiquitin ligase activator of NFKB 1 (Mul1), nuclear gene encoding mitochondrial protein | 10 ug |
$757.00
|
|
| MR205346L2 | Lenti ORF clone of Mul1 (mGFP-tagged) - Mouse mitochondrial ubiquitin ligase activator of NFKB 1 (Mul1), nuclear gene encoding mitochondrial protein | 10 ug |
$757.00
|
|
| MR205346L3 | Lenti ORF clone of Mul1 (Myc-DDK-tagged) - Mouse mitochondrial ubiquitin ligase activator of NFKB 1 (Mul1), nuclear gene encoding mitochondrial protein | 10 ug |
$757.00
|
|
| MR205346L4 | Lenti ORF clone of Mul1 (mGFP-tagged) - Mouse mitochondrial ubiquitin ligase activator of NFKB 1 (Mul1), nuclear gene encoding mitochondrial protein | 10 ug |
$757.00
|
|
Citations
Mul1 suppresses Nlrp3 inflammasome activation through ubiquitination and degradation of Asc
Kim, Lee, Suh et al
BioRxiv (2019)
Kim, Lee, Suh et al
BioRxiv (2019)
Snippet: Plasmids of the wild-type mouse Mul1 (pMul1-FLAG) and Asc (pAsc-Myc) were constructed using pCMV6 Mul1-Myc/DDK (MR205346, Origene, Rockville, MD, USA) and pcDNA3-N-FLAG-Asc (a gift from Bruce Beutler, #75134, Addgene, Watertown, MA, USA) as templates, respectively. Plasmids of RING domain mutant Mul1
Showing 1-1 of 1 papers.
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