Apoh (NM_013475) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205186
Apoh (Myc-DDK-tagged) - Mouse apolipoprotein H (Apoh)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Apoh
Synonyms B2GPI; beta-2-GPI; beta2-GPI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205186 representing NM_013475
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTTCCCCGGTGCTCGCCTTGTTCTCCGCCTTCCTCTGCCATGTTGCTATTGCAGGACGGATCTGTC
CGAAGCCGGATGACCTACCATTTGCTACGGTTGTCCCCTTAAAGACATCCTACGACCCTGGGGAGCAGAT
TGTCTACTCCTGCAAGCCAGGCTACGTGTCCAGGGGAGGGATGAGACGGTTTACCTGTCCTCTCACAGGA
ATGTGGCCCATCAACACCCTGAGATGTGTCCCCAGAGTATGTCCTTTCGCTGGAATCTTAGAAAATGGAA
TTGTACGCTACACGAGTTTTGAATATCCCAAGAACATCAGTTTTGCTTGTAACCCTGGGTTTTTTCTGAA
TGGGACCAGCTCATCTAAGTGCACGGAGGAAGGAAAATGGAGCCCAGATATTCCTGCTTGTGCTCGCATC
ACCTGCCCGCCACCACCAGTTCCAAAGTTTGCACTCCTTAAGGATTATAGGCCTTCAGCTGGGAACAACT
CTTTGTATCAGGACACAGTGGTCTTTAAATGCTTGCCACACTTTGCCATGATCGGAAATGACACAGTCAT
GTGCACAGAACAAGGAAACTGGACCCGATTGCCAGAATGCCTGGAAGTAAAATGTCCCTTCCCTCCGAGG
CCAGAAAATGGGTATGTGAATTATCCTGCAAAGCCGGTGCTTCTATATAAGGATAAAGCCACATTTGGTT
GCCATGAGACATACAAGCTGGACGGCCCAGAAGAAGCGGAATGTACCAAGACGGGAACTTGGTCCTTCTT
GCCGACCTGTAGAGAGTCTTGCAAACTCCCCGTTAAGAAAGCCACCGTGCTGTACCAAGGGATGAGGGTG
AAGATCCAGGAACAGTTTAAGAATGGGATGATGCATGGCGACAAAATTCACTTCTACTGCAAAAACAAAG
AGAAGAAGTGCAGCTACACTGTGGAGGCTCATTGCAGAGATGGCACTATCGAGATTCCCTCGTGCTTCAA
GGAGCACAGTTCTCTGGCTTTCTGGAAAACGGATGCATCAGAACTGACACCGTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205186 representing NM_013475
Red=Cloning site Green=Tags(s)

MVSPVLALFSAFLCHVAIAGRICPKPDDLPFATVVPLKTSYDPGEQIVYSCKPGYVSRGGMRRFTCPLTG
MWPINTLRCVPRVCPFAGILENGIVRYTSFEYPKNISFACNPGFFLNGTSSSKCTEEGKWSPDIPACARI
TCPPPPVPKFALLKDYRPSAGNNSLYQDTVVFKCLPHFAMIGNDTVMCTEQGNWTRLPECLEVKCPFPPR
PENGYVNYPAKPVLLYKDKATFGCHETYKLDGPEEAECTKTGTWSFLPTCRESCKLPVKKATVLYQGMRV
KIQEQFKNGMMHGDKIHFYCKNKEKKCSYTVEAHCRDGTIEIPSCFKEHSSLAFWKTDASELTPC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013475
ORF Size 1035 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013475.4
RefSeq Size 1165 bp
RefSeq ORF 1038 bp
Locus ID 11818
UniProt ID Q01339
Cytogenetics 11 71.8 cM
MW 39.1 kDa
Summary Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013475" in other vectors (4)
SKU Description Size Price
MC202287 Apoh (untagged) - Mouse apolipoprotein H (Apoh), (10ug) 10 ug
$457.00
MG205186 Apoh (tGFP-tagged) - Mouse apolipoprotein H (Apoh) 10 ug
$657.00
MR205186L3 Lenti ORF clone of Apoh (Myc-DDK-tagged) - Mouse apolipoprotein H (Apoh) 10 ug
$757.00
MR205186L4 Lenti ORF clone of Apoh (mGFP-tagged) - Mouse apolipoprotein H (Apoh) 10 ug
$757.00

Plasmid Map for MR205186

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Cloning scheme for MR205186

Circular map for MR205186

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