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Tob2 (NM_020507) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR205181
Tob2 (Myc-DDK-tagged) - Mouse transducer of ERBB2, 2 (Tob2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tob2
Synonyms 2900090N22Rik; 4930545K18Rik; AV071822; mKIAA1663
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205181 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCTGGAGATCAAAGTGGCCCTCAACTTCATCATCTCCTACTTATACAACAAGCTGCCCCGCCGCA
GGGCAGACCTGTTTGGGGAGGAGCTAGAGCGGCTTCTGAGAAAGAAATATGAGGGCCACTGGTACCCGGA
GAAGCCGTTGAAGGGTTCTGGCTTCCGCTGTGTCCACATTGGGGAAGTGGTAGACCCTGTGGTGGAGCTG
GCTGCCAAGCGGAGCGGCCTAGCTGTGGAGGATGTGCGTGCCAACGTGCCCGAAGAGCTGAGTGTCTGGA
TTGACCCTTTCGAGGTATCCTACCAGATTGGCGAGAAGGGAGTCGTGAAGGTCCTGTACCTGGGTGACAG
TGAGGCGAGTGGTACCCCAGAGCTGGACAAAGAGATCAAGAGCAGCTTCAACCCTGACGCCCAGGTGTTT
GTGCCCATCGGCAGCCAGGACAGCTCTCTGTCCAGCTCTCCCTCGCCGTCCTTTGGCCAGTCACCCAGCC
CCACCTTCATCCCCCGGTCTGCCCAGCCCATCACCTTTACTACTGCCTCTTTTGCTGCCACCAAATTTGG
TTCCACTAAGATGAAGAAGGGTGGTGGGGCATCTGGCGGTGTGAGTGTGGGTGGCAGCGGGGCGGGTGGC
CAGCAGCCGCCGCCACAGCAGCCTCGCATGGCCCGCTCACCCACAAAGAACCTGCTGAAGCACAAGAGCC
TCTCTCTATCTATGCATTCACTGAACCTCATCCCAGCCAATCCGGCCCCCCAGTCCCAGCTCTCCCCCAA
TGCCAAGGAGTTTGTGTACAACGGTGGTGGCTCACCCAGCCTCTTCTTCGATGGGGTGGAAGGCCCGAGC
ACCAGCACCACGGCCCCCTTCGGCAGCGGTGGAGCCAGCACTTGCAACAGCAGCAGCTTTGATGTGTCCC
AGGTGTTCGGAGGCGGTGCTAACAGCCTGTTCTTGGAGAAATCGCCCTTTGTGGAAGGCCTCAGCTACAA
CCTGAATACCATGCAGTATCCCAACCAGCCCTTCCAGCCTGTCGTGCTGGCCAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR205181 protein sequence
Red=Cloning site Green=Tags(s)

MQLEIKVALNFIISYLYNKLPRRRADLFGEELERLLRKKYEGHWYPEKPLKGSGFRCVHIGEVVDPVVEL
AAKRSGLAVEDVRANVPEELSVWIDPFEVSYQIGEKGVVKVLYLGDSEASGTPELDKEIKSSFNPDAQVF
VPIGSQDSSLSSSPSPSFGQSPSPTFIPRSAQPITFTTASFAATKFGSTKMKKGGGASGGVSVGGSGAGG
QQPPPQQPRMARSPTKNLLKHKSLSLSMHSLNLIPANPAPQSQLSPNAKEFVYNGGGSPSLFFDGVEGPS
TSTTAPFGSGGASTCNSSSFDVSQVFGGGANSLFLEKSPFVEGLSYNLNTMQYPNQPFQPVVLAN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020507
ORF Size 1035 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020507.3, NP_065253.1
RefSeq Size 3994 bp
RefSeq ORF 1038 bp
Locus ID 57259
UniProt ID Q9JM55
Cytogenetics 15 E1
MW 36.8 kDa
Summary Anti-proliferative protein inhibits cell cycle progression from the G0/G1 to S phases.UniProtKB/Swiss-Prot Function
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"NM_020507" in other vectors (4)
SKU Description Size Price
MC203801 Tob2 (untagged) - Mouse transducer of ERBB2, 2 (Tob2), (10ug) 10 ug
$457.00
MG205181 Tob2 (tGFP-tagged) - Mouse transducer of ERBB2, 2 (Tob2) 10 ug
$657.00
MR205181L3 Lenti ORF clone of Tob2 (Myc-DDK-tagged) - Mouse transducer of ERBB2, 2 (Tob2) 10 ug
$757.00
MR205181L4 Lenti ORF clone of Tob2 (mGFP-tagged) - Mouse transducer of ERBB2, 2 (Tob2) 10 ug
$757.00

Plasmid Map for MR205181

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR205181
Circular map for MR205181

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