Dcaf7 (NM_027946) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205133
Dcaf7 (Myc-DDK-tagged) - Mouse DDB1 and CUL4 associated factor 7 (Dcaf7)
$289.00 $457.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dcaf7
Synonyms 1700012F10Rik; 2610037L01Rik; C86529; HAN11; Wdr68
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205133 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCTGCACGGCAAACGGAAGGAGATCTACAAATATGAAGCGCCCTGGACTGTGTACGCCATGAACT
GGAGCGTGCGGCCGGACAAGCGCTTTCGCCTGGCGCTGGGCAGCTTCGTGGAAGAGTACAACAACAAGGT
TCAGCTTGTTGGTTTAGATGAGGAGAGTTCAGAGTTTATTTGCCGAAACACCTTTGACCACCCGTACCCT
ACCACAAAGCTCATGTGGATTCCTGACACAAAGGGCGTCTATCCAGACCTCCTGGCAACAAGTGGTGACT
ATCTCCGTGTGTGGAGGGTTGGTGAAACAGAGACCAGGCTGGAGTGCTTGCTAAACAACAACAAGAACTC
AGACTTCTGTGCTCCCCTGACCTCCTTTGACTGGAACGAGGTGGATCCTTATCTTCTAGGTACCTCAAGC
ATTGACACAACGTGCACCATCTGGGGACTAGAGACCGGGCAAGTGCTGGGACGAGTGAATCTCGTGTCTG
GCCATGTGAAGACCCAGCTTGCCCATGACAAAGAGGTCTATGACATCGCCTTCAGCCGCGCTGGAGGGGG
CAGGGACATGTTTGCCTCTGTGGGCGCTGATGGGTCTGTGAGAATGTTTGACCTCCGCCATCTGGAACAT
AGCACCATCATTTATGAAGACCCACAGCACCACCCCCTGCTTCGCCTCTGCTGGAACAAGCAGGACCCTA
ACTACCTGGCCACCATGGCCATGGACGGGATGGAGGTGGTGATTCTGGATGTTCGAGTTCCCTGCACACC
GGTTGCTAGGCTAAACAACCACCGAGCATGTGTCAATGGCATTGCCTGGGCCCCACACTCGTCCTGCCAC
ATCTGTACTGCAGCGGATGACCATCAGGCCCTCATCTGGGACATCCAGCAGATGCCTCGGGCCATCGAGG
ACCCTATCTTGGCCTACACAGCTGAGGGAGAGATCAACAATGTGCAGTGGGCCTCCACTCAGCCTGACTG
GATCGCTATCTGCTACAACAACTGCCTGGAGATACTCCGCGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205133 protein sequence
Red=Cloning site Green=Tags(s)

MSLHGKRKEIYKYEAPWTVYAMNWSVRPDKRFRLALGSFVEEYNNKVQLVGLDEESSEFICRNTFDHPYP
TTKLMWIPDTKGVYPDLLATSGDYLRVWRVGETETRLECLLNNNKNSDFCAPLTSFDWNEVDPYLLGTSS
IDTTCTIWGLETGQVLGRVNLVSGHVKTQLAHDKEVYDIAFSRAGGGRDMFASVGADGSVRMFDLRHLEH
STIIYEDPQHHPLLRLCWNKQDPNYLATMAMDGMEVVILDVRVPCTPVARLNNHRACVNGIAWAPHSSCH
ICTAADDHQALIWDIQQMPRAIEDPILAYTAEGEINNVQWASTQPDWIAICYNNCLEILRV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027946
ORF Size 1023 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027946.1, NM_027946.2, NM_027946.3, NP_082222.1
RefSeq Size 5765 bp
RefSeq ORF 1029 bp
Locus ID 71833
UniProt ID P61963
Cytogenetics 11 E1
MW 38.8 kDa
Summary Involved in craniofacial development. Acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches. Associates with DIAPH1 and controls GLI1 transcriptional activity. Could be involved in skin development. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027946" in other vectors (3)
SKU Description Size Price
MG205133 Dcaf7 (tGFP-tagged) - Mouse WD repeat domain 68 (Wdr68) 10 ug
$657.00
MR205133L3 Lenti ORF clone of Dcaf7 (Myc-DDK-tagged) - Mouse DDB1 and CUL4 associated factor 7 (Dcaf7) 10 ug
$757.00
MR205133L4 Lenti ORF clone of Dcaf7 (mGFP-tagged) - Mouse DDB1 and CUL4 associated factor 7 (Dcaf7) 10 ug
$757.00

Plasmid Map for MR205133

Download sequence for

Cloning scheme for MR205133

Circular map for MR205133

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