Steap1 (NM_027399) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205069
Steap1 (Myc-DDK-tagged) - Mouse six transmembrane epithelial antigen of the prostate 1 (Steap1)
$289.00 $457.00
2 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Steap1
Synonyms 2410007B19Rik; Prss24; Steap
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205069 representing NM_027399
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGATCAGTGACGATGTTACAAACCCAGAACAACTTTGGAAAATGAAGCCAAAGGGAAACCTGGAAG
ATGACAGTTACTCGACTAAGGACTCGGGAGAGACGAGCATGCTGAAAAGACCGGGGCTCTCGCACTTGCA
GCACGCGGTCCACGTCGATGCCTTTGACTGCCCCTCCGAGCTTCAGCACACGCAGGAATTCTTTCCAAAC
TGGCGCTTGCCAGTGAAAGTCGCTGCCATCATATCATCCCTGACTTTCCTGTACACACTTCTGAGGGAAA
TCATCTACCCGTTAGTAACTTCCCGTGAACAATATTTTTATAAAATTCCAATCCTGGTTATTAACAAAGT
CTTGCCAATGGTCGCCATTACCCTCTTGGCACTGGTCTATTTGCCAGGAGAGTTAGCGGCAGTTGTACAG
CTTCGCAATGGAACCAAGTACAAGAAGTTCCCACCCTGGTTAGATAGATGGATGCTGGCCAGGAAGCAGT
TTGGTCTCCTCAGCTTCTTTTTTGCTGTGCTGCACGCTGTTTACAGTCTCTCATACCCAATGAGGAGATC
CTACAGATACAAGCTACTCAACTGGGCTTACAAACAGGTTCAACAAAACAAAGAGGATGCCTGGGTAGAG
CATGATGTCTGGAGAATGGAGATTTATGTGTCCCTGGGGATTGTGGGGCTGGCCATCTTGGCTCTCTTGG
CTGTGACATCTATTCCATCTGTGAGCGACTCTTTAACCTGGAGAGAATTTCACTATATTCAGAGCAAACT
AGGAATTGTCTCTCTTCTTCTGGGCACAGTACACGCTTTGGTTTTTGCCTGGAATAAATGGGTAGATGTC
AGTCAATTTGTATGGTACATGCCTCCGACTTTTATGATAGCTGTTTTCCTTCCAACTCTTGTCCTGATAT
GTAAAATCGCCCTGTGCCTGCCCTGTCTGAGGAAGAAGATACTGAAGATTAGATGTGGTTGGGAAGATGT
CAGCAAAATTAACAGGACTGAGATGGCCTCCAGGTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205069 representing NM_027399
Red=Cloning site Green=Tags(s)

MEISDDVTNPEQLWKMKPKGNLEDDSYSTKDSGETSMLKRPGLSHLQHAVHVDAFDCPSELQHTQEFFPN
WRLPVKVAAIISSLTFLYTLLREIIYPLVTSREQYFYKIPILVINKVLPMVAITLLALVYLPGELAAVVQ
LRNGTKYKKFPPWLDRWMLARKQFGLLSFFFAVLHAVYSLSYPMRRSYRYKLLNWAYKQVQQNKEDAWVE
HDVWRMEIYVSLGIVGLAILALLAVTSIPSVSDSLTWREFHYIQSKLGIVSLLLGTVHALVFAWNKWVDV
SQFVWYMPPTFMIAVFLPTLVLICKIALCLPCLRKKILKIRCGWEDVSKINRTEMASRL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027399
ORF Size 1017 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027399.3, NP_081675.2
RefSeq Size 1216 bp
RefSeq ORF 1020 bp
Locus ID 70358
UniProt ID Q9CWR7
Cytogenetics 5 3.2 cM
MW 39.7 kDa
Summary Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027399" in other vectors (4)
SKU Description Size Price
MC211059 Steap1 (untagged) - Mouse six transmembrane epithelial antigen of the prostate 1 (Steap1), (10ug) 10 ug
$457.00
MG205069 Steap1 (tGFP-tagged) - Mouse six transmembrane epithelial antigen of the prostate 1 (Steap1) 10 ug
$657.00
MR205069L3 Lenti ORF clone of Steap1 (Myc-DDK-tagged) - Mouse six transmembrane epithelial antigen of the prostate 1 (Steap1) 10 ug
$757.00
MR205069L4 Lenti ORF clone of Steap1 (mGFP-tagged) - Mouse six transmembrane epithelial antigen of the prostate 1 (Steap1) 10 ug
$757.00

Plasmid Map for MR205069

Download sequence for

Cloning scheme for MR205069

Circular map for MR205069

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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