Gpr65 (NM_008152) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205027
Gpr65 (Myc-DDK-tagged) - Mouse G-protein coupled receptor 65 (Gpr65)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gpr65
Synonyms Dig1; Gpcr25; TDAG8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205027 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGATGAACAGCATGTGCATTGAAGAGCAGCACCACCTCGAGCACTATTTGTTCCCGGTGGTCTACA
TAATTGTGTTTATAGTCAGCGTCCCAGCCAACATCGGATCTTTATGCGTATCCTTTCTGCAAGCGAAGAA
GGAAAATGAGCTAGGAATTTACCTCTTCAGTCTGTCCCTGTCAGACCTGCTGTATGCGCTGACTCTGCCC
CTCTGGATCAATTACACTTGGAATAAAGACAACTGGACTTTCTCTCCCACTTTGTGCAAAGGAAGCGTTT
TCTTCACCTACATGAACTTTTATAGCAGCACAGCGTTCCTCACTTGCATTGCCCTGGACCGCTATTTAGC
AGTCGTCTACCCTCTGAAGTTTTCCTTCCTAAGAACGAGAAGATTCGCGTTTATTACCAGCCTCTCTATC
TGGATATTAGAGTCCTTCTTTAACTCTATGCTTCTGTGGAAAGATGAAACGAGTGTTGAATATTGTGACT
CGGACAAATCTAATTTCACTCTCTGCTATGACAAATACCCTCTGGAGAAATGGCAGATAAACCTCAACCT
GTTTCGGACGTGCATGGGCTACGCAATACCCTTGATCACCATCATGATCTGCAACCATAAAGTCTACCGA
GCTGTGCGGCACAACCAAGCCACGGAAAACAGCGAGAAGAGAAGGATCATAAAGTTGCTTGCTAGCATCA
CGTTGACTTTCGTCCTATGCTTTACCCCCTTCCACGTGATGGTGCTCATCCGCTGCGTTTTAGAGCGCGA
CATGAACGTCAATGACAAGTCTGGATGGCAGACGTTTACGGTGTACAGAGTCACAGTGGCCCTGACGAGT
CTAAACTGTGTTGCCGATCCCATTCTGTACTGCTTTGTGACTGAGACGGGGAGAGCTGATATGTGGAACA
TATTAAAATTGTGTACTAGGAAACACAATAGACACCAAGGACAAAAAAGGGACATACTTTCTGTGTCCAC
AAGAGATGCTGTAGAATTAGAGATTATAGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205027 protein sequence
Red=Cloning site Green=Tags(s)

MAMNSMCIEEQHHLEHYLFPVVYIIVFIVSVPANIGSLCVSFLQAKKENELGIYLFSLSLSDLLYALTLP
LWINYTWNKDNWTFSPTLCKGSVFFTYMNFYSSTAFLTCIALDRYLAVVYPLKFSFLRTRRFAFITSLSI
WILESFFNSMLLWKDETSVEYCDSDKSNFTLCYDKYPLEKWQINLNLFRTCMGYAIPLITIMICNHKVYR
AVRHNQATENSEKRRIIKLLASITLTFVLCFTPFHVMVLIRCVLERDMNVNDKSGWQTFTVYRVTVALTS
LNCVADPILYCFVTETGRADMWNILKLCTRKHNRHQGQKRDILSVSTRDAVELEIID

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008152
ORF Size 1011 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008152.1, NM_008152.2, NM_008152.3, NP_032178.1
RefSeq Size 2153 bp
RefSeq ORF 1014 bp
Locus ID 14744
UniProt ID Q61038
Cytogenetics 12 E
MW 39.4 kDa
Summary Receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids (By similarity). Plays a role in immune response by maintaining lysosome function and supporting phagocytosis-mediated intracellular bacteria clearance (PubMed:27287411). May have a role in activation-induced cell death or differentiation of T-cells (PubMed:8599842).UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_008152" in other vectors (4)
SKU Description Size Price
MC204259 Gpr65 (untagged) - Mouse G-protein coupled receptor 65 (Gpr65), (10ug) 10 ug
$457.00
MG205027 Gpr65 (tGFP-tagged) - Mouse G-protein coupled receptor 65 (Gpr65) 10 ug
$489.00 $657.00
MR205027L3 Lenti ORF clone of Gpr65 (Myc-DDK-tagged) - Mouse G-protein coupled receptor 65 (Gpr65) 10 ug
$757.00
MR205027L4 Lenti ORF clone of Gpr65 (mGFP-tagged) - Mouse G-protein coupled receptor 65 (Gpr65) 10 ug
$757.00

Plasmid Map for MR205027

Download sequence for

Cloning scheme for MR205027

Circular map for MR205027

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products