Dbx1 (NM_001005232) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204981
Dbx1 (Myc-DDK-tagged) - Mouse developing brain homeobox 1 (Dbx1)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dbx1
Synonyms AI426026; Dbx
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204981 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGTTCCCCGGCCTCCTTGCGCCTCCCGCGGGATACCCTAGCCTCCTGCGACCCACGCCCACCTTAA
CACTGCCCCAGTCCCTTCAATCCGCATTTTCCGGCCACTCTAGTTTCCTAGTAGAAGATTTGATCCGCAT
CAGCCGGCCTCCCACCTACCTGTCCCGCAGCATACCCGCTGCCAGCCTGTCACCCCCCAGTCAAGAGGCC
CCTGCAGCCCTCGCCGACTCGGGGACCTCAGACCTGGGCTCTCCTGGGTCCGGGAGCCGGCGAGGCAGTT
CTCCACAGACCGCCCTCTCCCCTGCCAGCGAGCCCACGTTTCTGAAGTTTGGGGTGAATGCCATCCTTTC
CTCGGCGCCTAGAAGAGAAACTTCGCCCGCTTTGCTGCAGAGCCCTCCTCCCAAGACGTTCGCCTTTCCA
TACTTCGAAGGCTCGTTCCAACCCTTTATCAGATCCTCCTATTTCCCAGCTTCCTCCAGCGTCGTGCCTA
TCCCTGGGACCTTCTCCTGGCCGCTGGCTGCTCGCGGCAAGCCTCGCCGGGGCATGCTGCGCAGAGCCGT
GTTCTCTGACGTGCAGCGGAAAGCCCTGGAGAAGACGTTCCAGAAGCAGAAGTACATCAGCAAGCCAGAC
CGGAAAAAGCTGGCCTCCAAGCTTGGCTTGAAGGACTCGCAGGTGAAAATCTGGTTCCAGAATCGACGCA
TGAAATGGCGGAACTCCAAAGAGCGCGAGCTCCTGTCTAGCGGGGGCTGCCGAGAGCAGACCCTTCCCAC
AAAACTAAACCCCCATCCAGACCTTAGTGATGTAGGCCAGAAGGGACCTGGGGATGAGGAGGAAGACAAT
CCGGGTGCCCGCCTGGCCTACCACGCGCCCGCAGACCCGCGACATTTGCTGGAAGGGCCACTGCCTGCCT
CGCCGGCGCACTCGAGCAGCCCGGGTAAACCGTCAGACTTCTCTGATTCTGATGAGGATGAGGAGGGCGA
GGAGGATGAGGAAATCACGGTGTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204981 protein sequence
Red=Cloning site Green=Tags(s)

MMFPGLLAPPAGYPSLLRPTPTLTLPQSLQSAFSGHSSFLVEDLIRISRPPTYLSRSIPAASLSPPSQEA
PAALADSGTSDLGSPGSGSRRGSSPQTALSPASEPTFLKFGVNAILSSAPRRETSPALLQSPPPKTFAFP
YFEGSFQPFIRSSYFPASSSVVPIPGTFSWPLAARGKPRRGMLRRAVFSDVQRKALEKTFQKQKYISKPD
RKKLASKLGLKDSQVKIWFQNRRMKWRNSKERELLSSGGCREQTLPTKLNPHPDLSDVGQKGPGDEEEDN
PGARLAYHAPADPRHLLEGPLPASPAHSSSPGKPSDFSDSDEDEEGEEDEEITVS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001005232
ORF Size 1005 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001005232.1, NP_001005232.1
RefSeq Size 2096 bp
RefSeq ORF 1008 bp
Locus ID 13172
UniProt ID P52950
Cytogenetics 7 31.44 cM
MW 36.3 kDa
Summary Could have a role in patterning the central nervous system during embryogenesis. Has a key role in regulating the distinct phenotypic features that distinguish two major classes of ventral interneurons, V0 and V1 neurons. Regulates the transcription factor profile, neurotransmitter phenotype, intraspinal migratory path and axonal trajectory of V0 neurons, features that differentiate them from an adjacent set of V1 neurons.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001005232" in other vectors (4)
SKU Description Size Price
MC207258 Dbx1 (untagged) - Mouse developing brain homeobox 1 (Dbx1), (10ug) 10 ug
$503.00
MG204981 Dbx1 (tGFP-tagged) - Mouse developing brain homeobox 1 (Dbx1) 10 ug
$657.00
MR204981L3 Lenti ORF clone of Dbx1 (Myc-DDK-tagged) - Mouse developing brain homeobox 1 (Dbx1) 10 ug
$757.00
MR204981L4 Lenti ORF clone of Dbx1 (mGFP-tagged) - Mouse developing brain homeobox 1 (Dbx1) 10 ug
$757.00

Plasmid Map for MR204981

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Cloning scheme for MR204981

Circular map for MR204981

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