Cops5 (NM_013715) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR204941
Cops5 (Myc-DDK-tagged) - Mouse COP9 (constitutive photomorphogenic) homolog, subunit 5 (Arabidopsis thaliana) (Cops5)
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Cops5 |
| Synonyms | AI303502; CSN5; Jab1; Mov34; Sgn5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_013715 |
| ORF Size | 1002 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_013715.2, NP_038743.1 |
| RefSeq Size | 1273 bp |
| RefSeq ORF | 1005 bp |
| Locus ID | 26754 |
| UniProt ID | O35864 |
| Cytogenetics | 1 2.29 cM |
| MW | 38 kDa |
| Summary | Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. Promotes the proteasomal degradation of BRSK2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_013715" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC209743 | Cops5 (untagged) - Mouse COP9 (constitutive photomorphogenic) homolog, subunit 5 (Arabidopsis thaliana) (Cops5), (10ug) | 10 ug |
$732.00
|
|
| MG204941 | Cops5 (tGFP-tagged) - Mouse COP9 (constitutive photomorphogenic) homolog, subunit 5 (Arabidopsis thaliana) (Cops5) | 10 ug |
$886.00
|
|
| MR204941L3 | Lenti ORF clone of Cops5 (Myc-DDK-tagged) - Mouse COP9 (constitutive photomorphogenic) homolog, subunit 5 (Arabidopsis thaliana) (Cops5) | 10 ug |
$986.00
|
|
| MR204941L4 | Lenti ORF clone of Cops5 (mGFP-tagged) - Mouse COP9 (constitutive photomorphogenic) homolog, subunit 5 (Arabidopsis thaliana) (Cops5) | 10 ug |
$986.00
|
|
Citations
ROS disrupt COP9 signalosome-mediated ABCA1 protection and trigger its ubiquitination and degradation by cullin3 inhibiting cholesterol efflux and promoting foam cell formation in response to GPCR agonists
Govatati, Yadavalli, Traylor et al
Redox Biol (2026) 91, 104095 DOI: 10.1016/j.redox.2026.104095
Govatati, Yadavalli, Traylor et al
Redox Biol (2026) 91, 104095 DOI: 10.1016/j.redox.2026.104095
Cited by 3
AI Summary: OriGene's MycDDK-tagged pCMV-CSN5 plasmid (MR204941) was used in this study to overexpress CSN5, enabling the authors to demonstrate that increased CSN5 levels inhibit GPCR agonist-induced ubiquitination and degradation of ABCA1, thereby restoring cholesterol efflux and reducing foam cell formation.
Snippet: OxLDL (BT910) was purchased from Alfa Aesar (Tweksbury, MA). MycDDK-tagged pCMV-CSN5 plasmid (MR204941) was bought from Origene (Rockville, MD). Anti-ubiquitin antibody (P4D1) was purchased from Enzo Life Sciences (Farmingdale, NY). Control non-targeting siRNA (D-001810-10), mouse Gα12 siRNA
Showing 1-1 of 1 papers.
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