Gfi1b (NM_008114) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR204861
Gfi1b (Myc-DDK-tagged) - Mouse growth factor independent 1B (Gfi1b), transcript variant 1
$289.00
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Gfi1b |
| Synonyms | Gfi-1B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_008114 |
| ORF Size | 990 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_008114.3 |
| RefSeq Size | 1724 bp |
| RefSeq ORF | 993 bp |
| Locus ID | 14582 |
| UniProt ID | O70237 |
| Cytogenetics | 2 A3 |
| MW | 37.4 kDa |
| Summary | Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_008114" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC201880 | Gfi1b (untagged) - Mouse growth factor independent 1B (Gfi1b), transcript variant 1, (10ug) | 10 ug |
$300.00
|
|
| MG204861 | Gfi1b (tGFP-tagged) - Mouse growth factor independent 1B (Gfi1b) | 10 ug |
$500.00
|
|
| MR204861L3 | Lenti ORF clone of Gfi1b (Myc-DDK-tagged) - Mouse growth factor independent 1B (Gfi1b), transcript variant 1 | 10 ug |
$600.00
|
|
| MR204861L4 | Lenti ORF clone of Gfi1b (mGFP-tagged) - Mouse growth factor independent 1B (Gfi1b), transcript variant 1 | 10 ug |
$600.00
|
|
Citations
Transcriptional deregulation of oncogenic myocyte enhancer factor 2C in T-cell acute lymphoblastic leukemia
Nagel, Venturini, Meyer et al
Leuk. Lymphoma (2011) 52 (2), 290-7 DOI: 10.3109/10428194.2010.537003
Nagel, Venturini, Meyer et al
Leuk. Lymphoma (2011) 52 (2), 290-7 DOI: 10.3109/10428194.2010.537003
Cited by 31
AI Summary: OriGene products provided expression constructs for GFI1B, HOXA9, and HOXA10, as well as cloned cDNAs for LYL1 and TAL1, which were used to investigate the transcriptional regulation of MEF2C in T-cell acute lymphoblastic leukemia.
Snippet: Expression constructs for GFI1B, HOXA9, and HOXA10 and cloned cDNAs for LYL1 and TAL1 were obtained from Origene (Rockville, MD, USA). ... that MEF2C is repressed by STAT5, suggest that the IL7-JAK-STAT5 pathway physiologically contributes to the down-regulation of
Showing 1-2 of 2 papers.
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