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Gfi1b (NM_008114) Mouse Untagged Clone

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SKU
MC201880
Gfi1b (untagged) - Mouse growth factor independent 1B (Gfi1b), transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Gfi1b
Synonyms Gfi-1B
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC052654 sequence for NM_008114
AAGAGGTAATCCGACTCATAACGTTGACCGAGCCGAGAGCAGTCCCCAGGGACAGTGTGGAGGTTCGTGG CTCTCGGGCAGAACTCAGAAGAGGGACAGCTCCCTGACGGTGTGGCGTGCACGCAGAAAAATGCCACGGT CCTTTCTAGTGAAGAGTAAGAAGGCACACACTTACCACCAGCCCCGGGCACAGGGTGATGAGCTGGTCTG GCCTCCTGCTGTAATTCCTGTGGCAAAAGAGCATAGCCAGAGTGCCAGCCCTCTTCTCAGCACACCGCTT CCAAGCCAGACCTTGGACTGGAACACAATCAAACAGGAGCGGGAGATGTTGCTGAACCAGAGCCTTCCCA AGATGGCCTCAGCCCCAGAGGGGCCTCTCGTGACACCCCAACCCCAGGATGGGGAATCACCACTCTCTGA GTCACCCCCTTTCTACAAGCCCAGCTTCTCCTGGGATACCTTGGCCTCCTCCTACAGCCACAGCTACACA CAGACCCCCTCCACCATGCAGTCCGCCTTCCTGGAGCGCTCCGTGAGGCTGTACGGCAGCCCCCTCGTGC CCAGCACAGAGTCTCCCTTGGACTTCCGCCTCCGCTACTCTCCAGGCATGGACACTTACCACTGTGTCAA GTGCAACAAGGTGTTCTCCACCCCTCATGGGCTAGAAGTGCATGTCCGCCGCTCTCACAGCGGAACCCGG CCCTTTGCCTGTGATGTCTGTGGCAAAACCTTTGGCCACGCTGTGAGCTTGGAGCAGCATACTCACGTCC ACTCACAGGAGCGAAGCTTCGAGTGCCGGATGTGTGGCAAAGCCTTCAAGCGTTCATCCACCCTGTCCAC CCACCTGCTCATCCACTCGGACACTCGGCCCTACCCCTGCCAGTTCTGTGGGAAGCGCTTCCACCAGAAG TCGGACATGAAGAAACACACCTACATCCACACAGGTGAGAAGCCCCACAAGTGCCAGGTGTGTGGGAAAG CCTTCAGCCAGAGCTCCAACCTCATCACCCACAGCCGCAAGCACACAGGCTTCAAGCCGTTCAGCTGTGA GCTGTGCACCAAGGGCTTCCAGCGCAAGGTGGACCTGCGACGTCACCGTGAGAGTCAACACAATCTCAAG TGAGACGGTTGGCCGCCTGCTTTAGTGTGTCTCGCCTGAAGGCCAGCCTCTCCTTTCCAATCCTGATCCC AGTCCTCCTGGAAGCAGCATTGCCCATGAGCCTCTCTGCTTCTTTTGAGACTGGATGACCTCAACCAAGC CACACTCCTCCTCTGACCACAGCTGATGTGGGAGTCAGTTCGGGCCTTTCTGAACTGAGGCAGTCACAGA CATGCTCTGGTCTCATTCAATGCCAGAGCACAGACAGCTACAAAGCCCTCGTGGACATGGCAAAACCAGG CAAGAGCCAGGATCCACAGAGAAACCCTCTTGCTTGCTTCTCTGGGTCTAGTGACTGAATTTTTAGGGAT TCTCTTTTTGGCTGCCACAGTCAGAGTTGTCCCCTGGCCTAGAGGTCTGGCGCCCCTCTGCTTCGGCCAG ATGTGCTCTCTGTCCCCCACCTCTCACCACATCCATCCGCCCACTGGGAGTCAGGCTCCTGAGATGAGCC ACGAGGCTATGAAATCAGCCATGATAGTAGAAGCCTGCTCCTATCAGTTGATGTAAATGTAAATAAAGGT GGGTTTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_008114
Insert Size 993 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC052654, AAH52654
RefSeq Size 1795 bp
RefSeq ORF 993 bp
Locus ID 14582
UniProt ID O70237
Cytogenetics 2 A3
Summary Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes a dominant isoform (1).
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"NM_008114" in other vectors (4)
SKU Description Size Price
MG204861 Gfi1b (tGFP-tagged) - Mouse growth factor independent 1B (Gfi1b) 10 ug
$500.00
MR204861 Gfi1b (Myc-DDK-tagged) - Mouse growth factor independent 1B (Gfi1b), transcript variant 1 10 ug
$289.00 $300.00
MR204861L3 Lenti ORF clone of Gfi1b (Myc-DDK-tagged) - Mouse growth factor independent 1B (Gfi1b), transcript variant 1 10 ug
$600.00
MR204861L4 Lenti ORF clone of Gfi1b (mGFP-tagged) - Mouse growth factor independent 1B (Gfi1b), transcript variant 1 10 ug
$600.00

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