Ppa2 (NM_146141) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204852
Ppa2 (Myc-DDK-tagged) - Mouse pyrophosphatase (inorganic) 2 (Ppa2), nuclear gene encoding mitochondrial protein
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ppa2
Synonyms 1110013G13Rik; Sid6306
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204852 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCGCGCTGCTGCCGCTGCTGAGCGTCGGCCGAGGGTGGCGCGTGGGGGCCGCCGCCCGCCCTCCGC
GCCGCGTCATGTCCCTGTACCGCACGGAGGAGCTGGGCCATCCCCGCTCGCAGGACTACCGCCTGTTCTT
CAAGCATGTAGCTGGTCATTACATCTCCCCTTTCCACGACATTCCTCTGAAGGCGGACTGTAAAGAGGAA
CATGACATTCCCAGGAAAAAGGCACGAAATGATGAATATGAGAATTTGTTTAATATGGTTGTGGAAATAC
CTCGGTGGACAAATGCCAAGATGGAGATTGCCACAGAGGAGCCACTCAATCCCATTAAGCAAGATATAAA
GAACGGAAAGCTACGCTACACACCGAACATCTTCCCTCACAAGGGCTATATATGGAATTACGGGGCCCTC
CCTCAGACCTGGGAAGATCCTCACCTAAGAGATAAGAGCACCGACTGCTGTGGGGATAATGATCCCATTG
ACGTCTGTGAAATAGGCTCAAAGGTTCTTTCCCGTGGAGACGTGGTCCATGTGAAGATCCTTGGGACTTT
GGCACTTATTGATCAGAGTGAAACAGATTGGAAAATAATTGCTATCAATGTGAATGATCCTGAGGCTGAA
AAATTTCATGATATTGATGATGTTAAGAAGTTCAAACCAGGTTACCTGGAAGCCACTCTCAATTGGTTTA
GACTCTATAAGGTACCAGATGGAAAACCAGAAAACAAGTTTGCTTTTAATGGAGAATTCAAAAACAAGGC
TTTCGCCCTCGACGTTATTAACTCTGCACATGAACGTTGGAAAGAGATGGTTATGAAGAAGTGTGACAAG
GGAGCCATCAGTTGTGTAAATGTGCACATCTGCGATAGCCCTTTCCACTGCACTATGGAGGAAGCAAGAT
CATTGGTTGAATCGGTTCCAACACCTTCGATGAATAAGGAAAGCAATGTAGAGGAAGAAGTGTGGCACTT
CCTCCGCAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204852 protein sequence
Red=Cloning site Green=Tags(s)

MRALLPLLSVGRGWRVGAAARPPRRVMSLYRTEELGHPRSQDYRLFFKHVAGHYISPFHDIPLKADCKEE
HDIPRKKARNDEYENLFNMVVEIPRWTNAKMEIATEEPLNPIKQDIKNGKLRYTPNIFPHKGYIWNYGAL
PQTWEDPHLRDKSTDCCGDNDPIDVCEIGSKVLSRGDVVHVKILGTLALIDQSETDWKIIAINVNDPEAE
KFHDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENKFAFNGEFKNKAFALDVINSAHERWKEMVMKKCDK
GAISCVNVHICDSPFHCTMEEARSLVESVPTPSMNKESNVEEEVWHFLRN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146141
ORF Size 990 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146141.2, NP_666253.1
RefSeq Size 1383 bp
RefSeq ORF 993 bp
Locus ID 74776
UniProt ID Q91VM9
Cytogenetics 3 G3
MW 38.1 kDa
Summary Hydrolyzes inorganic pyrophosphate. This activity is essential for correct regulation of mitochondrial membrane potential, and mitochondrial organization and function.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_146141" in other vectors (4)
SKU Description Size Price
MC207746 Ppa2 (untagged) - Mouse pyrophosphatase (inorganic) 2 (Ppa2), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$330.00
MG204852 Ppa2 (tGFP-tagged) - Mouse pyrophosphatase (inorganic) 2 (Ppa2) 10 ug
$500.00
MR204852L3 Lenti ORF clone of Ppa2 (Myc-DDK-tagged) - Mouse pyrophosphatase (inorganic) 2 (Ppa2), nuclear gene encoding mitochondrial protein 10 ug
$600.00
MR204852L4 Lenti ORF clone of Ppa2 (mGFP-tagged) - Mouse pyrophosphatase (inorganic) 2 (Ppa2), nuclear gene encoding mitochondrial protein 10 ug
$600.00

Plasmid Map for MR204852

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Cloning scheme for MR204852

Circular map for MR204852

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