Ptgr1 (NM_025968) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204840
Ptgr1 (Myc-DDK-tagged) - Mouse prostaglandin reductase 1 (Ptgr1)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ptgr1
Synonyms 2510002C21Rik; Ltb4dh; Zadh3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204840 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCAGGCCAAGAGCTGGACCCTGAAGAAGCACTTCGAAGGCTTCCCTACGGACGGTAACTTCGAGT
TGAAGACGACTGAGCTCCCACCCTTAAATAATGGAGAGGTCCTGCTGGAAGCCCTGTTCCTCTCTGTGGA
TCCTTACATGAGAGTTGCAGCCAAAAAACTGAAGGAGGGCGACAGGATGATGGGCGAGCAAGTGGCCAGA
GTCGTGGAAAGTAAAAACTCAGCCTTCCCAAAAGGAACGATTGTAGCGGCTTTATTAGGTTGGACATCAC
ATTCCATTTCTGATGGGAATGGACTGACAAAGCTGCCTGTAGAGTGGCCGGACAAGTTGCCACTGTCTTT
GGCACTGGGGACAGTTGGCATGCCAGGCCTCACTGCCTACTTTGGCCTGCTTGACATCTGTGGCGTGAAG
GGTGGAGAAACCGTGATGGTCAGCGCAGCAGCAGGAGCAGTGGGCTCTGTTGTGGGGCAGATAGCTAAGC
TCAAGGGCTGCAAAGTTGTTGGTACAGCAGGGTCTGATGAAAAGGTTGCCTATCTTAAGAAGCTTGGATT
CGACGTGGCCTTTAACTACAAGACAGTAAAGTCACTGGAGGAAGCTTTGAGGACAGCCTCTCCCGATGGT
TATGATTGCTATTTTGATAATGTAGGTGGAGAGTTTTCAAACGCTGTTATACTCCAGATGAAGACATTTG
GAAGGATTGCCATCTGTGGTGCCATCTCTCAATACAACCGTACTGGACCATGTCCCCAGGGCCCAGCCCC
AGAGGTGGTTATCTATCAGCAACTCCGCATGGAGGGGTTCATCGTGAATCGGTGGCAAGGAGAAGTCCGC
CAGAAGGCTCTGACAGAGTTGATGAATTGGGTCTCAGAGGGTAAAGTCCAGTATCATGAACACGTCACTG
AAGGATTTGAGAAAATGCCCGCAGCATTTATGGGAATGTTGAAAGGAGAAAATCTGGGGAAGACCATAGT
GAAAGCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204840 protein sequence
Red=Cloning site Green=Tags(s)

MVQAKSWTLKKHFEGFPTDGNFELKTTELPPLNNGEVLLEALFLSVDPYMRVAAKKLKEGDRMMGEQVAR
VVESKNSAFPKGTIVAALLGWTSHSISDGNGLTKLPVEWPDKLPLSLALGTVGMPGLTAYFGLLDICGVK
GGETVMVSAAAGAVGSVVGQIAKLKGCKVVGTAGSDEKVAYLKKLGFDVAFNYKTVKSLEEALRTASPDG
YDCYFDNVGGEFSNAVILQMKTFGRIAICGAISQYNRTGPCPQGPAPEVVIYQQLRMEGFIVNRWQGEVR
QKALTELMNWVSEGKVQYHEHVTEGFEKMPAAFMGMLKGENLGKTIVKA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025968
ORF Size 987 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025968.1, NM_025968.2, NM_025968.3, NP_080244.1
RefSeq Size 2104 bp
RefSeq ORF 990 bp
Locus ID 67103
UniProt ID Q91YR9
Cytogenetics 4 B3
MW 35.6 kDa
Summary Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-oxo-PGE1, 15-oxo-PGE2 and 15-oxo-PGE2-alpha. Has no activity towards PGE1, PGE2 and PGE2-alpha. Catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4. This is an initial and key step of metabolic inactivation of leukotriene B4 (By similarity).UniProtKB/Swiss-Prot Function
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"NM_025968" in other vectors (4)
SKU Description Size Price
MC203699 Ptgr1 (untagged) - Mouse prostaglandin reductase 1 (Ptgr1), (10ug) 10 ug
$300.00
MG204840 Ptgr1 (tGFP-tagged) - Mouse leukotriene B4 12-hydroxydehydrogenase (Ltb4dh) 10 ug
$500.00
MR204840L3 Lenti ORF clone of Ptgr1 (Myc-DDK-tagged) - Mouse prostaglandin reductase 1 (Ptgr1) 10 ug
$600.00
MR204840L4 Lenti ORF clone of Ptgr1 (mGFP-tagged) - Mouse prostaglandin reductase 1 (Ptgr1) 10 ug
$600.00

Plasmid Map for MR204840

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Cloning scheme for MR204840

Circular map for MR204840

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