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Ldah (NM_172401) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204760
1110057K04Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110057K04 gene (1110057K04Rik), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ldah
Synonyms 1110057K04Rik; mLDAH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204760 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCAGAAGTCGAGGAACAAATCCCTGTGCGTGAGGAGTTCTTTCTCTGTGGTGGAGTTGAAACCA
AGATCATAAAATGTGGGCCCTGGACTAACCTCTTTGAGAAACAAGACGTCAGCAAGCCAAAACAGCTTAT
CTTCATCATTCCCGGTAACCCAGGCTATTCCGCATTCTACGTGCCATTTGCAAAGGCCTTGTACACTTTG
ATGAAGAGCCGCTTCCCGGTATGGATAATCTCTCATGCTGGGTTTTCGGTGACTCCCAAAGACAAGAAGG
TTCTTGCAGCACCACAGGAAGAGTCAAATGCTCAAAAAATTGAGGACGTCTATGGTCTGAATGGTCAAAT
AGAGCACAAAATAGCTTTTCTGAGAGCTCACGTGCCAAAGGATGTGAAGCTTATACTCATTGGCCATTCC
GTAGGCACCTATATGACCCTTCACGTGATGAAGCGAGTCCCCGAGCTGCCTGTCGCCCACGCCTTTCTGC
TCTTCCCAACTATCGAACGAATGTCTGAATCGCCCAACGGCAAGTTTGCCACTCCATTTTTGTGCCAGTT
TCGATACCTGCTCTATGCTACCAGCTACTTACTTTTTAAGCCGTGTCCTGAAGTCATAAAATCTTTCATA
ATCCAAAAGCTCATGGGACAAATGAACATTAAGCTTGAACTTCCTTTGACGGATATCCTTCAACCATTTT
GCCTTGCTAACGCTGCCTACCTCGGGAGCCAAGAAATGGTCCAGATAGTGAAGCGAGATGATGACATCAT
AAAGGAGTTTCTCCCTAAGCTTAAGTTTTACTATGGTAAAACTGATGGCTGGTGTCCAGTAAAGTACTAT
GAAGACATGAAGAAAGATTTTCCAGAAGGCAATATTTACCTCTGTGAGAAAGGAATACCACATGCTTTTG
TTCTCGATTTTAGCCAGGAGATGGCTACCATAGTTGCTGAGTGGATAAATAATAGACCGCCCAGAAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204760 protein sequence
Red=Cloning site Green=Tags(s)

MASEVEEQIPVREEFFLCGGVETKIIKCGPWTNLFEKQDVSKPKQLIFIIPGNPGYSAFYVPFAKALYTL
MKSRFPVWIISHAGFSVTPKDKKVLAAPQEESNAQKIEDVYGLNGQIEHKIAFLRAHVPKDVKLILIGHS
VGTYMTLHVMKRVPELPVAHAFLLFPTIERMSESPNGKFATPFLCQFRYLLYATSYLLFKPCPEVIKSFI
IQKLMGQMNIKLELPLTDILQPFCLANAAYLGSQEMVQIVKRDDDIIKEFLPKLKFYYGKTDGWCPVKYY
EDMKKDFPEGNIYLCEKGIPHAFVLDFSQEMATIVAEWINNRPPRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172401
ORF Size 978 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172401.5
RefSeq Size 2757 bp
RefSeq ORF 981 bp
Locus ID 68832
UniProt ID Q8BVA5
Cytogenetics 12 A1.1
MW 37.4 kDa
Summary Serine lipid hydrolase associated with lipid droplets. Highly expressed in macrophage-rich areas in atherosclerotic lesions, suggesting that it could promote cholesterol ester turnover in macrophages.UniProtKB/Swiss-Prot Function
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"NM_172401" in other vectors (4)
SKU Description Size Price
MC207670 1110057K04Rik (untagged) - Mouse RIKEN cDNA 1110057K04 gene (1110057K04Rik), transcript variant 1, (10ug) 10 ug
$330.00
MG204760 1110057K04Rik (tGFP-tagged) - Mouse RIKEN cDNA 1110057K04 gene (1110057K04Rik) 10 ug
$500.00
MR204760L3 Lenti ORF clone of 1110057K04Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110057K04 gene (1110057K04Rik), transcript variant 1 10 ug
$600.00
MR204760L4 Lenti ORF clone of 1110057K04Rik (mGFP-tagged) - Mouse RIKEN cDNA 1110057K04 gene (1110057K04Rik), transcript variant 1 10 ug
$600.00

Plasmid Map for MR204760

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204760
Circular map for MR204760

file-alt Citations

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