Ankrd9 (NM_175207) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204746
Ankrd9 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 9 (Ankrd9)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ankrd9
Synonyms 2500003O20Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204746 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGTGGGACACCAGGCCTGGACGCAGCGCCAACGGAGGGCCCGAGGGTCCCGGCGCAGCTCGCTTGC
GGGTGCAGAAGCAGTGCCGGAAGTCATCCTTCGCCTTCTACCTGGCAGTGCGAGATCAGCTGCCAGTTTG
GCTACTCGAGGACATACGTGCCAGCGAAGCCTTCCACTGTGATGAGCGGGGGCGCGCTGCGGCCTATTCG
CCTTCGGAGGCTCTGCTCTATGCGCTTGTGCACGACCACCAAGCCTATGCACACTACCTGCTAGCTACCT
TCCCGCGGTGTGCACTCGCCCCACCTAGCGCGGGCTTCCGCTGCTGCACCGCGCCTGGGCCTCACGTGGC
GCTGGCGGTGCGCTACAACCGCGTGGGCATCCTGCGGCGCATCCTGCGAACGGTGCAGGACTTCCCAGTG
GAAGAGCGCGTGCGCCTCCTCGACCGCCGTGGCTGCAGCCGCGTGGAGGGCGGTGGCACGTCGCTGCATG
TGGCCTGTGAGCTCGCACGCCCCGAGTGCCTCTTTCTGCTGCTTGGCCACGGAGCTTCTCCAGGCCTTCG
AGATGGCAGTGGCTTTACACCATTGGAGCTGCTGTTGCGCCAGCTGAACCAGGACGCCAGCTCAGCCCCC
ACTAAGGCTGAGGCAGCTTCTGCCACGGTCAATGCTGCCACCGCCAACACCACTTCGTCTGAGGAGGTGT
GTCAGCGCCGCCTGCTGCTGCTTGACCTGCTAGTACTGTACACCCCGGGGGGCGTCGTAGGTCCAGCTCG
ATGTGAACTGCTAGGAGACCAGCTACGCTGGCAGCGGTTGTTGGGTGAGGACAAGTTCCAGTGGCTGGCA
GGGCTGGCGCCACCCTCCCTCTTTGTCCGTGCCATGCAGGTGTTGGTCACCACTATCTCACCTGGCCGCT
TCCCGGAGGCCTTAGATGAACTGCCTCTGCCATCGTTTTTGCAGCCCTTGGACCTCACGGGCAAGGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204746 protein sequence
Red=Cloning site Green=Tags(s)

MPWDTRPGRSANGGPEGPGAARLRVQKQCRKSSFAFYLAVRDQLPVWLLEDIRASEAFHCDERGRAAAYS
PSEALLYALVHDHQAYAHYLLATFPRCALAPPSAGFRCCTAPGPHVALAVRYNRVGILRRILRTVQDFPV
EERVRLLDRRGCSRVEGGGTSLHVACELARPECLFLLLGHGASPGLRDGSGFTPLELLLRQLNQDASSAP
TKAEAASATVNAATANTTSSEEVCQRRLLLLDLLVLYTPGGVVGPARCELLGDQLRWQRLLGEDKFQWLA
GLAPPSLFVRAMQVLVTTISPGRFPEALDELPLPSFLQPLDLTGKG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175207
ORF Size 978 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175207.5
RefSeq Size 2558 bp
RefSeq ORF 981 bp
Locus ID 74251
UniProt ID Q8BH83
Cytogenetics 12 F1
MW 35.5 kDa
Summary Substrate receptor subunit of a cullin-RING superfamily E3 ligase complex (CUL5-based E3 ubiquitin ligase complex) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Depending of the metabolic state of the cell, promotes the proteasomal degradation of IMPDH2, the rate-limiting enzyme in GTP biosynthesis or protects IMPDH2 by stabilizing IMPDH2 filaments assembly. Implicated in different cellular processes, like copper homeostasis and cell proliferation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175207" in other vectors (4)
SKU Description Size Price
MC201701 Ankrd9 (untagged) - Mouse ankyrin repeat domain 9 (Ankrd9), (10ug) 10 ug
$300.00
MG204746 Ankrd9 (tGFP-tagged) - Mouse ankyrin repeat domain 9 (Ankrd9) 10 ug
$500.00
MR204746L3 Lenti ORF clone of Ankrd9 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 9 (Ankrd9) 10 ug
$600.00
MR204746L4 Lenti ORF clone of Ankrd9 (mGFP-tagged) - Mouse ankyrin repeat domain 9 (Ankrd9) 10 ug
$600.00

Plasmid Map for MR204746

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Cloning scheme for MR204746

Circular map for MR204746

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