Spdef (NM_013891) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204725
Spdef (Myc-DDK-tagged) - Mouse SAM pointed domain containing ets transcription factor (Spdef)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Spdef
Synonyms PDEF; Pse
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204725 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAGTGCCAGCCCAGGCCTGAGCAACGTGTCCCCCGGTTGCCTGCTACTGTTCCCAGATGTGGCAC
CACGAACAGGGACGGAGAAGGCAGCATCAGGAGCAATGGGCCCTGAGAAGCAGGAATGGAGTCCTAGTCC
ACCCGCCACCCCTGAGCAGGGCCTGTCTGCTTTCTACCTCTCTTACTTTAACATGTATCCCGACGATAGC
AGCTGGGTCGCCAAAGTCCCCGAGGCCCGTGCCGGGGAGGACCACCCGGAGGAGCCCGAGCAGTGTCCCG
TCATTGACAGCCAGGCCTCTGGGAGCACGTTGGATGAGCACTCGCTAGAGCAGGTGCAATCGATGGTTGT
GGGCGAGGTCCTGAAAGATATTGAGACGGCCTGCAAGCTTCTGAACATCACAGCAGACCCTGGGGACTGG
AGCCCTGGTAACGTGCAGAAGTGGCTTTTATGGACAGAACACCAGTACCGGCTGCCTCCAGCAGGCAAGG
CCTTCCAGGAGCTGGGCGGTAAGGAGCTGTGCGCCATGTCCGAGGAACAGTTCCGTCAGCGTGCACCCTT
GGGTGGGGATGTACTGCATGCCCACCTGGACATCTGGAAGTCAGCGGCCTGGATGAAGGAGAGGACCTCG
CCTGGGACCCTTCACTACTGCGCCTCCACCAGCGAGGAGGGCTGGACGGATGGTGAGGTGGACTCGTCGT
GCTCCGGGCAGCCCATTCACCTGTGGCAGTTCCTGAAAGAACTGCTGCTCAAGCCCCACAGCTATGGCCG
CTTCATCCGCTGGCTCAACAAGGAGAAAGGCATCTTCAAAATTGAGGACTCAGCACAGGTGGCCCGACTG
TGGGGTGTGCGCAAGAACCGGCCAGCCATGAACTATGATAAACTAAGCCGCTCCATCCGCCAGTATTACA
AGAAGGGCATCATTCGTAAACCCGACATCTCTCAGCGCCTTGTCTACCAATTTGTGCATCCAGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204725 protein sequence
Red=Cloning site Green=Tags(s)

MGSASPGLSNVSPGCLLLFPDVAPRTGTEKAASGAMGPEKQEWSPSPPATPEQGLSAFYLSYFNMYPDDS
SWVAKVPEARAGEDHPEEPEQCPVIDSQASGSTLDEHSLEQVQSMVVGEVLKDIETACKLLNITADPGDW
SPGNVQKWLLWTEHQYRLPPAGKAFQELGGKELCAMSEEQFRQRAPLGGDVLHAHLDIWKSAAWMKERTS
PGTLHYCASTSEEGWTDGEVDSSCSGQPIHLWQFLKELLLKPHSYGRFIRWLNKEKGIFKIEDSAQVARL
WGVRKNRPAMNYDKLSRSIRQYYKKGIIRKPDISQRLVYQFVHPV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013891
ORF Size 975 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013891.4
RefSeq Size 1824 bp
RefSeq ORF 978 bp
Locus ID 30051
UniProt ID Q9WTP3
Cytogenetics 17 A3.3
MW 36.4 kDa
Summary May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013891" in other vectors (4)
SKU Description Size Price
MC200856 Spdef (untagged) - Mouse SAM pointed domain containing ets transcription factor (Spdef), (10ug) 10 ug
$300.00
MG204725 Spdef (tGFP-tagged) - Mouse SAM pointed domain containing ets transcription factor (Spdef) 10 ug
$500.00
MR204725L3 Lenti ORF clone of Spdef (Myc-DDK-tagged) - Mouse SAM pointed domain containing ets transcription factor (Spdef) 10 ug
$600.00
MR204725L4 Lenti ORF clone of Spdef (mGFP-tagged) - Mouse SAM pointed domain containing ets transcription factor (Spdef) 10 ug
$600.00

Plasmid Map for MR204725

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Cloning scheme for MR204725

Circular map for MR204725

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