Tfec (NM_031198) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204566
Tfec (Myc-DDK-tagged) - Mouse transcription factor EC (Tcfec)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tfec
Synonyms BB107417; bHLHe34; Tcfec
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204566 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCTTTGACTGTCGGGTATGCGACCAGACTCTCAAGAGAGCGCAGCCTCCAGCAGCCAGCTGCATGC
CTCTGGCAGAGCATGAACCCATGAGCCCAGACAGTGATGCTGGCTGTGCAGGAAACCCATTCACAAATCT
GCTGGCTCTTGGGAAAAAAGATGGTGCTGAGAAATGGCATTTATCTGGGAGTATTTTGGATGTGTACAGT
GATGAGCAGGGCATTTCTTCAGCTAATGCTGGTCTCACGGATGCTCCTTGTCCAAGTATTCTACCCATGA
GAAAGGAAATTGCAGAAACTGATGGTAGAGCTTTGGCAAAGGAGAGACAGAAAAAGGACAACCACAACCT
CATTGAAAGAAGAAGAAGGTATAATATTAATTACCGAATCAAGGAGCTTGGCACTCTTATTCCAAAGTCT
AATGATCCTGATATGCGCTGGAACAAAGGGACCATTCTAAAGGCATCAGTGGACTACATCAAGTGGCTAC
AAAAAGAACAACAGAGAGCCCGGGAGTTAGAACACAGACAGAAGAAATTAGAACATGCTAACCGTCAGCT
TAGGCTCCGGATTCAGGAACTAGAAATACAAGCACGTGCTCATGGTCTACCAATCCTGGCTTCACTCGGC
ACAGCTGATGTTGGTACCCACATCACCAAGCAGCAGACCCATCCTGAGAGGAACTTAGGAGGCTGTTGCT
TACAACTGACTCCAACTCAGGGGACAAGCCCGGAGTTCTATGAGCAAGCTGTGGCTTTCTCTGATCCATT
GTCTCACTTCACAGATTTATCCTTTAGTGCCGCTTTGAAAGAGGAACAAAGATTGGATGGCATGCTACTA
AGTGATACAATATGCCCCTTTGGGACGGACCCACTACTCTCTGCCATTTCCCCTGCAGTTTCCAAAGCTA
GCAGCCGAAGCAGCCTTAGCTCAGAGGATGGTGATGAGTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204566 protein sequence
Red=Cloning site Green=Tags(s)

MTFDCRVCDQTLKRAQPPAASCMPLAEHEPMSPDSDAGCAGNPFTNLLALGKKDGAEKWHLSGSILDVYS
DEQGISSANAGLTDAPCPSILPMRKEIAETDGRALAKERQKKDNHNLIERRRRYNINYRIKELGTLIPKS
NDPDMRWNKGTILKASVDYIKWLQKEQQRARELEHRQKKLEHANRQLRLRIQELEIQARAHGLPILASLG
TADVGTHITKQQTHPERNLGGCCLQLTPTQGTSPEFYEQAVAFSDPLSHFTDLSFSAALKEEQRLDGMLL
SDTICPFGTDPLLSAISPAVSKASSRSSLSSEDGDEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_031198
ORF Size 951 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031198.3, NP_112475.1
RefSeq Size 1779 bp
RefSeq ORF 954 bp
Locus ID 21426
UniProt ID Q9WTW4
Cytogenetics 6 A2
MW 35.1 kDa
Summary Transcriptional regulator that acts as a repressor or an activator. Acts as a transcriptional transactivator on the proximal promoter region of the tartrate-resistant acid phosphatase (TRAP) E-box containing promoter. Collaborates with MITF in target gene activation. Acts as a transcriptional repressor on minimal promoter containing element F (that includes an E-box sequence) (By similarity). Binds to element F in an E-box sequence-specific manner (By similarity). Acts as a transcriptional repressor on minimal promoter containing mu E3 enhancer sequence (By similarity). Binds to mu E3 DNA sequence of the immunoglobulin heavy-chain gene enhancer (By similarity). Binds DNA in a homo- or heterodimeric form.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_031198" in other vectors (4)
SKU Description Size Price
MC207417 Tfec (untagged) - Mouse transcription factor EC (Tcfec), (10ug) 10 ug
$330.00
MG204566 Tfec (tGFP-tagged) - Mouse transcription factor EC (Tcfec) 10 ug
$500.00
MR204566L3 Lenti ORF clone of Tfec (Myc-DDK-tagged) - Mouse transcription factor EC (Tcfec) 10 ug
$600.00
MR204566L4 Lenti ORF clone of Tfec (mGFP-tagged) - Mouse transcription factor EC (Tcfec) 10 ug
$600.00

Plasmid Map for MR204566

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Cloning scheme for MR204566

Circular map for MR204566

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