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Prkra (NM_011871) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204457
Prkra (Myc-DDK-tagged) - Mouse protein kinase, interferon inducible double stranded RNA dependent activator (Prkra)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Prkra or NM_011871"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Prkra
Synonyms AV120107; lear; Pact; PRK; RAX
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204457 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCATAGCAGGCATCGTGCCGAGGCCCCGCCGCTGCAGCGCGAGGACAGCGGGACCTTCAGTTTGG
GCAAGATGATAACAGCTAAGCCTGGGAAAACACCGATTCAGGTATTGCACGAGTACGGCATGAAGACCAA
GAACATTCCAGTTTATGAATGTGAAAGATCCGATGTGCAAGTACACGTGCCCACTTTCACCTTCAGAGTA
ACCGTTGGTGACATAACTTGCACAGGTGAAGGTACAAGTAAGAAGCTGGCGAAGCACAGAGCTGCGGAGG
CCGCCATAAACATTTTGAAAGCCAATGCAAGTATTTGCTTTGCAGTTCCTGACCCCTTAATGCCTGATCC
ATCCAAACAGCCGAAGAACCAGCTGAATCCAATTGGCTCATTACAGGAATTAGCAATTCACCATGGCTGG
CGACTTCCTGAATATACCCTTTCCCAGGAAGGAGGACCGGCTCATAAGAGGGAATACACCACGATCTGCA
GGCTTGAATCATTCATGGAAACTGGAAAGGGGGCATCAAAAAAACAAGCCAAGAGAAATGCTGCTGAGAA
ATTTCTCGCCAAGTTTAGTAATATTTCTCCAGAGAACCACATTTCTCTAACGAACGTGGTTGGACATTCC
CTAGGATGCACTTGGCACTCCTTGAGGAATTCCCCTGGTGAGAAGATCAACCTCCTGAAAAGGAGCCTCC
TCAGTCTCCCGAACACAGACTACATCCAGCTGCTTAGTGAGATTGCCAGCGAGCAAGGCTTTAACATAAC
GTATTTGGATATAGAGGAGCTGAGTGCCAACGGACAGTATCAGTGTCTCGCAGAGCTGTCCACCAGTCCT
ATCACCGTGTGTCACGGCTCAGGCATCTCCTGTGGCAATGCACAGAGTGACGCTGCTCACAATGCTCTGC
AGTATTTAAAGATAATAGCAGAAAGAAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204457 protein sequence
Red=Cloning site Green=Tags(s)

MSHSRHRAEAPPLQREDSGTFSLGKMITAKPGKTPIQVLHEYGMKTKNIPVYECERSDVQVHVPTFTFRV
TVGDITCTGEGTSKKLAKHRAAEAAINILKANASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGW
RLPEYTLSQEGGPAHKREYTTICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHS
LGCTWHSLRNSPGEKINLLKRSLLSLPNTDYIQLLSEIASEQGFNITYLDIEELSANGQYQCLAELSTSP
ITVCHGSGISCGNAQSDAAHNALQYLKIIAERK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011871
ORF Size 939 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011871.3
RefSeq Size 1554 bp
RefSeq ORF 942 bp
Locus ID 23992
UniProt ID Q9WTX2
Cytogenetics 2 C3
MW 34.4 kDa
Summary Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1 (By similarity). Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner.UniProtKB/Swiss-Prot Function
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"NM_011871" in other vectors (4)
SKU Description Size Price
MC203800 Prkra (untagged) - Mouse protein kinase, interferon inducible double stranded RNA dependent activator (Prkra), (10ug) 10 ug
$300.00
MG204457 Prkra (tGFP-tagged) - Mouse protein kinase, interferon inducible double stranded RNA dependent activator (Prkra) 10 ug
$500.00
MR204457L3 Lenti ORF clone of Prkra (Myc-DDK-tagged) - Mouse protein kinase, interferon inducible double stranded RNA dependent activator (Prkra) 10 ug
$600.00
MR204457L4 Lenti ORF clone of Prkra (mGFP-tagged) - Mouse protein kinase, interferon inducible double stranded RNA dependent activator (Prkra) 10 ug
$600.00

Plasmid Map for MR204457

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204457
Circular map for MR204457

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