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Crym (NM_016669) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204445
Crym (Myc-DDK-tagged) - Mouse crystallin, mu (Crym)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Crym
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204445 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCGGGCGCCAGCGTTCCTGAGCGCAGAGGAGGTGCAGGATCACCTTCGCAGCTCCAGCCTTCTCA
TCCCACCCCTGGAGGCCGCACTGGCCAACTTCTCCAAAGGTCCCGACGGAGGGGTCATGCAGCCAGTGCG
CACCGTGGTGCCTGTAGCCAAGCACCGAGGCTTCCTGGGAGTCATGCCTGCCTACAGTGCTGCTGAGGAT
GCGCTCACCACCAAGTTAGTCACCTTCTATGAGGGCCACAGCAACACAGCGGTCCCCTCCCATCAGGCAT
CGGTGCTTCTCTTTGATCCCAGCAATGGCTCCCTGCTGGCGGTCATGGATGGAAATGTCATAACTGCAAA
GAGAACAGCAGCGGTGTCTGCCATTGCCACAAAGCTGTTGAAGCCCCCAGGCAGTGATGTGCTGTGCATC
CTTGGAGCGGGGGTCCAGGCGTACAGTCACTATGAGATCTTCACAGAGCAGTTCTCCTTCAAGGAGGTGA
GAATGTGGAACCGCACCAGGGAAAATGCTGAGAAGTTTGCAAGCACAGTGCAAGGAGATGTTCGGGTCTG
TTCATCAGTGCAGGAGGCTGTGACAGGTGCTGATGTCATCATCACAGTCACCATGGCAACAGAGCCCATT
TTATTTGGTGAATGGGTAAAGCCGGGGGCTCACATCAATGCTGTTGGAGCCAGCAGGCCTGACTGGCGAG
AACTGGATGACGAGCTCATGAGGCAAGCGGTGCTGTATGTGGACTCCCGGGAGGCTGCCCTGAAGGAGTC
AGGAGACGTTCTGTTGTCAGGGGCTGACATCTTTGCTGAGCTTGGAGAAGTGATTTCAGGAGCGAAGCCT
GCACACTGTGAGAAGACCACAGTGTTCAAATCTTTGGGGATGGCAGTGGAAGACCTGGTTGCAGCCAAAT
TAGTATATGATTCTTGGTCATCTGGCAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204445 protein sequence
Red=Cloning site Green=Tags(s)

MKRAPAFLSAEEVQDHLRSSSLLIPPLEAALANFSKGPDGGVMQPVRTVVPVAKHRGFLGVMPAYSAAED
ALTTKLVTFYEGHSNTAVPSHQASVLLFDPSNGSLLAVMDGNVITAKRTAAVSAIATKLLKPPGSDVLCI
LGAGVQAYSHYEIFTEQFSFKEVRMWNRTRENAEKFASTVQGDVRVCSSVQEAVTGADVIITVTMATEPI
LFGEWVKPGAHINAVGASRPDWRELDDELMRQAVLYVDSREAALKESGDVLLSGADIFAELGEVISGAKP
AHCEKTTVFKSLGMAVEDLVAAKLVYDSWSSGK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016669
ORF Size 939 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016669.2
RefSeq Size 1256 bp
RefSeq ORF 942 bp
Locus ID 12971
UniProt ID O54983
Cytogenetics 7 64.47 cM
MW 33.5 kDa
Summary Specifically catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). Binds thyroid hormone which is a strong reversible inhibitor. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors (By similarity).UniProtKB/Swiss-Prot Function
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"NM_016669" in other vectors (4)
SKU Description Size Price
MC204467 Crym (untagged) - Mouse crystallin, mu (Crym), (10ug) 10 ug
$300.00
MG204445 Crym (tGFP-tagged) - Mouse crystallin, mu (Crym) 10 ug
$500.00
MR204445L3 Lenti ORF clone of Crym (Myc-DDK-tagged) - Mouse crystallin, mu (Crym) 10 ug
$600.00
MR204445L4 Lenti ORF clone of Crym (mGFP-tagged) - Mouse crystallin, mu (Crym) 10 ug
$600.00

Plasmid Map for MR204445

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204445
Circular map for MR204445

file-alt Citations

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