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Gpn2 (NM_133884) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204403
Gpn2 (Myc-DDK-tagged) - Mouse GPN-loop GTPase 2 (Gpn2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gpn2
Synonyms AI838661; Atpbd1b; R74630
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204403 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGGGCCGCCCCGACTACGGCCTTCGGGCAGGCAGTGATCGGGCCCCCGGGCTCGGGGAAGACCA
CGTACTGCCTGGGCATGAGTGAGTTCCTGCGCGCTCTGGGGCGGCGCGTGGCGGTGGTGAACCTGGACCC
CGCTAACGACGGACTGCCTTACGAGTGTGCGGTGGACGTGGGCGAGCTGGTGGGGCTGGGCGACGTGATG
GACGCGCTGCGCCTGGGGCCCAACGGTGGCCTGCTCTACTGCATGGAGTACCTGGAAGCCAACCTGGACT
GGCTGCGCGCCAAGCTGGAGCCCCTGCGAGGCCACTACTTTCTCTTCGACTGCCCCGGCCAGGTGGAGCT
CTGCACGCACCACACCGCCCTACGCAGCATCTTCTCCCAAATGGCCCAGTGGGACCTCAGGCTGACTGCT
GTCCACCTTGTGGATTCTCACTACTGCACAGACCCAGCCAAGTTCATCTCCGTCCTGTGTACCTCCCTGG
CCACCATGCTGCACGTGGAATTGCCCCACGTCAACCTCCTCTCCAAGATGGACCTTATTGAACACTATGG
GAAGCTGGCCTTCAACCTGGACTACTACACGGAGGTCCTGGACCTTTCCTACCTGCTTGAGCACCTGGCG
TCTGACCCTTTCTTCCGGCGCTACCGACAGCTCAATGAGAAGCTGGTGCAGCTGGTGGAAGACTACAGCC
TGGTCTCCTTCATCCCCCTGAACATCCAGGACAAGGACAGCATCCAGCGAGTCTTACAGGCTGTGGATAA
AGCTAATGGCTACTGTTTTGGGGTTCAAGAGCAGCGAAGCCTGGAAGCCATGATGTCCACTGCAATGGGA
GCTGATTTTCACTTCTCCTCCACTCTGGGCATCCAGGAGAAGTACTTAGCACCCTCGGAGCAGACCGCAG
AGCAGGAAGCCATGCAGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204403 protein sequence
Red=Cloning site Green=Tags(s)

MAGAAPTTAFGQAVIGPPGSGKTTYCLGMSEFLRALGRRVAVVNLDPANDGLPYECAVDVGELVGLGDVM
DALRLGPNGGLLYCMEYLEANLDWLRAKLEPLRGHYFLFDCPGQVELCTHHTALRSIFSQMAQWDLRLTA
VHLVDSHYCTDPAKFISVLCTSLATMLHVELPHVNLLSKMDLIEHYGKLAFNLDYYTEVLDLSYLLEHLA
SDPFFRRYRQLNEKLVQLVEDYSLVSFIPLNIQDKDSIQRVLQAVDKANGYCFGVQEQRSLEAMMSTAMG
ADFHFSSTLGIQEKYLAPSEQTAEQEAMQL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133884
ORF Size 930 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133884.1, NP_598645.1
RefSeq Size 1356 bp
RefSeq ORF 933 bp
Locus ID 100210
UniProt ID Q8VEJ1
Cytogenetics 4 D2.3
MW 34.6 kDa
Summary Small GTPase required for proper localization of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.UniProtKB/Swiss-Prot Function
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"NM_133884" in other vectors (4)
SKU Description Size Price
MC212054 Gpn2 (untagged) - Mouse GPN-loop GTPase 2 (Gpn2), (10ug) 10 ug
$330.00
MG204403 Gpn2 (tGFP-tagged) - Mouse ATP binding domain 1 family, member B (Atpbd1b) 10 ug
$500.00
MR204403L3 Lenti ORF clone of Gpn2 (Myc-DDK-tagged) - Mouse GPN-loop GTPase 2 (Gpn2) 10 ug
$600.00
MR204403L4 Lenti ORF clone of Gpn2 (mGFP-tagged) - Mouse GPN-loop GTPase 2 (Gpn2) 10 ug
$600.00

Plasmid Map for MR204403

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204403
Circular map for MR204403

file-alt Citations

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