Tlcd2 (NM_027249) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204395
Tlcd2 (Myc-DDK-tagged) - Mouse TLC domain containing 2 (Tlcd2)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tlcd2
Synonyms 2010305C02Rik; RP23-384C18.8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204395 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATCCTGGGGACTCCTTGTGGCTGGCGCTTCCTTCACGGCGTTTCGGGGACTGCACTGGGGGCTGC
AGCTGCTGCCCACCCCGAAATCTGTTCGGGACCGCTGGATGTGGCGGAACATTTTCGTTTCGCTGATACA
CAGCCTACTCTCTGGAGTAGGGGCGCTGGTCGGGCTGTGGCAGTTTCCTCAAATGGTCACCGACCCAATT
AATGATCACCCACCGTGGGCACGGGTCCTAGTAGCAGTGTCAGTGGGTTATTTCGCTGCAGATGGAGTTG
ATATGCTGTGGAACCAGACATTGGCCCAGGCCTGGGACCTTCTCTGTCACCATTTGGCGGTAGTGAGCTG
CCTCAGCACCGCTGTTGTGTCTGGCCACTATGTGGGCTTCTCTATGGTATCCCTGCTTCTGGAGCTGAAC
TCCATCTGTTTGCATCTACGGAAGCTACTGCTGCTCTCCCATAAGGCCCCATCCTTGGCCTTCAGAGTAA
GCAGTTGGGCCAGCCTGGCCACCCTGGTCCTCTTCCGCCTTCTGCCTCTGGGATGGATGAGTCTGTGGTT
GTCCCGGCAGCACTACCAGCTGTCTCTTGCTCTGGTTCTGCTTTGTGTGGCTGGGCTGGTCACCGTGGGC
AGCATAAGCATCTCCACAGGGATCCGAATTCTGACCAAGGATATCTTGCAGTCTCAGCCCTACCCGTTTA
TCCTCATGCACAAGGAAACCAAGACACGTGAGCCTGTTGCCAGGAACACTTCCACTCTCAGTCTGAAAGG
CTCCAGATACCTGTACTCCACTGCTGCTGCTGCTCTTGGTGGTCATCTCATGGTACTGGCATCTCCAAAA
CGCTGCATGACCCCTTCAGTCCTGGGCCTTCAAGAGAGAAGACTAGAGCCTGGCAAAGTGGCACATGCTG
ATAATGCTAGCACTTGGGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204395 protein sequence
Red=Cloning site Green=Tags(s)

MASWGLLVAGASFTAFRGLHWGLQLLPTPKSVRDRWMWRNIFVSLIHSLLSGVGALVGLWQFPQMVTDPI
NDHPPWARVLVAVSVGYFAADGVDMLWNQTLAQAWDLLCHHLAVVSCLSTAVVSGHYVGFSMVSLLLELN
SICLHLRKLLLLSHKAPSLAFRVSSWASLATLVLFRLLPLGWMSLWLSRQHYQLSLALVLLCVAGLVTVG
SISISTGIRILTKDILQSQPYPFILMHKETKTREPVARNTSTLSLKGSRYLYSTAAAALGGHLMVLASPK
RCMTPSVLGLQERRLEPGKVAHADNASTWE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027249
ORF Size 930 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027249.3, NP_081525.1
RefSeq Size 1424 bp
RefSeq ORF 933 bp
Locus ID 380712
UniProt ID Q8VC26
Cytogenetics 11 B5
MW 34.1 kDa
Summary Regulates the composition and fluidity of the plasma membrane (By similarity). Inhibits the incorporation of membrane-fluidizing phospholipids containing omega-3 long-chain polyunsaturated fatty acids (LCPUFA) and thereby promotes membrane rigidity (By similarity). Does not appear to have any effect on LCPUFA synthesis (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027249" in other vectors (4)
SKU Description Size Price
MC207964 Tlcd2 (untagged) - Mouse TLC domain containing 2 (Tlcd2), (10ug) 10 ug
$330.00
MG204395 Tlcd2 (tGFP-tagged) - Mouse RIKEN cDNA 2010305C02 gene (2010305C02Rik) 10 ug
$500.00
MR204395L3 Lenti ORF clone of Tlcd2 (Myc-DDK-tagged) - Mouse TLC domain containing 2 (Tlcd2) 10 ug
$600.00
MR204395L4 Lenti ORF clone of Tlcd2 (mGFP-tagged) - Mouse TLC domain containing 2 (Tlcd2) 10 ug
$600.00

Plasmid Map for MR204395

Download sequence for

Cloning scheme for MR204395

Circular map for MR204395

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