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Stx18 (BC021362) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204350
Stx18 (Myc-DDK-tagged) - Mouse syntaxin 18 (cDNA clone MGC:29151 IMAGE:5035350)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Stx18
Synonyms 1810035L21Rik; 4933425D03Rik; AU041562
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204350 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGTGGACATCACACTGCTCTTCCGGGCTAGCGTCAAGACAGTGAAGACGCGGAATAAGGCTCTGG
GAGTGGCCGTGGGTGGCGGGGCCGATGGCAGCCGGGACGAGCTGTTCCGCAGAAGCCCTCGGCCCAAGGG
AGACTTCTCCAGCCGGGCCCGTGAAGTGATTTCTCACATTGGGAAGCTGAGAGATTTTCTCCTGGAGCAC
AGGAAGGAGTATATTAATGCCTACAGCCACACCATGTCTGACTATGGGAGGATGACAGACACAGAGCGAG
ACCAGATAGACCAGGATGCTCAGATATTCATAAGGACCTGTTCAGAAGCAATCCAGCAACTCAGAACAGA
AGGAGTGTGTAAGCTTTACTCTGAACAGAGAGCCATCCGAGTGAAGCGCGTGGTGGACAAGAAAAGACTA
TCTAAGCTGGAACCTGAGCCACATACCAAGAGGAAAGACTCCACATCTGAGAAAGCCCCTCAGAACGTTT
CCCAGGACTCTGAAGGAAAGCCTGCTGCTGAGGAGCTGCCAGAAAAGCCTTTGGCTGAATCACAGCCCGA
ACTGGGAACCTGGGGCGATGGCAAGGGTGAAGATGAGCTGTCTCCAGAAGAGATACAGATGTTTGAACAA
GAAAATCAGCGACTCATTGGTGAAATGAACAGCTTGTTTGACGAAGTGAGGCAAATCGAAGGGAAAGTCG
TTGAAATTTCCAGACTCCAAGAGATATTCACCGAAAAGGTTTTGCAACAGGAAACTGAGATCGACAGCAT
TCACCAGTTAGTCGTGGGGGCAACTGAAAATATTAAGGAAGGCAACGAAGACATCCGAGAGGCCATCAAA
AACAATGCAGGTTTCCGAGTGTGGATCCTCTTCTTCCTCGTGATGTGTTCCTTTTCCTTGCTCTTCCTCG
ACTGGTACGACAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204350 protein sequence
Red=Cloning site Green=Tags(s)

MAVDITLLFRASVKTVKTRNKALGVAVGGGADGSRDELFRRSPRPKGDFSSRAREVISHIGKLRDFLLEH
RKEYINAYSHTMSDYGRMTDTERDQIDQDAQIFIRTCSEAIQQLRTEGVCKLYSEQRAIRVKRVVDKKRL
SKLEPEPHTKRKDSTSEKAPQNVSQDSEGKPAAEELPEKPLAESQPELGTWGDGKGEDELSPEEIQMFEQ
ENQRLIGEMNSLFDEVRQIEGKVVEISRLQEIFTEKVLQQETEIDSIHQLVVGATENIKEGNEDIREAIK
NNAGFRVWILFFLVMCSFSLLFLDWYDS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC021362
ORF Size 924 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC021362, AAH21362
RefSeq Size 1521 bp
RefSeq ORF 926 bp
Locus ID 71116
Cytogenetics 5 B3
MW 35.2 kDa
Summary Syntaxin that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER.UniProtKB/Swiss-Prot Function
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"BC021362" in other vectors (4)
SKU Description Size Price
MC217874 Stx18 (untagged) - Mouse syntaxin 18 (cDNA clone MGC:29151 IMAGE:5035350), (10ug) 10 ug
$330.00
MG204350 Stx18 (tGFP-tagged) - Mouse syntaxin 18 (cDNA clone MGC:29151 IMAGE:5035350) 10 ug
$500.00
MR204350L3 Lenti ORF clone of Stx18 (Myc-DDK-tagged) - Mouse syntaxin 18 (cDNA clone MGC:29151 IMAGE:5035350) 10 ug
$600.00
MR204350L4 Lenti ORF clone of Stx18 (mGFP-tagged) - Mouse syntaxin 18 (cDNA clone MGC:29151 IMAGE:5035350) 10 ug
$600.00

Plasmid Map for MR204350

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204350
Circular map for MR204350

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