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Ufd1 (NM_011672) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204334
Ufd1l (Myc-DDK-tagged) - Mouse ubiquitin fusion degradation 1 like (Ufd1l), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Ufd1 or NM_011672"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ufd1
Synonyms Ufd1; Ufd1l
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204334 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTTCTTTCAACATGTTTGACCACCCGATTCCCCGGGTCTTCCAGAACCGCTTCTCCACGCAGTACC
GCTGCTTCTCCGTGTCCATGCTAGCAGGGCCTAATGACAGGTCAGATGTGGAGAAAGGAGGGAAGATAAT
TATGCCACCCTCAGCCCTCGATCAACTCAGCCGGCTCAACATTACCTATCCTATGCTGTTTAAATTGACC
AATAAGAATTCAGATCGGATGACACACTGCGGTGTACTGGAGTTTGTTGCTGATGAAGGCATCTGTTACC
TCCCCCACTGGATGATGCAGAATTTGCTGTTGGAGGAAGGGGGCCTGGTTCAGGTGGAAAGTGTCAACCT
CCAAGTGGCGACCTACTCTAAGTTCCAGCCTCAGAGCCCAGACTTCCTGGATATTACCAACCCTAAAGCG
GTATTAGAAAATGCATTGAGAAACTTCGCCTGTCTGACGACTGGAGATGTGATTGCTATCAACTACAATG
AGAAGATCTATGAGCTGCGGGTGATGGAGACCAAACCTGACAAGGCTGTATCCATTATTGAATGTGACAT
GAATGTGGATTTTGATGCTCCCTTGGGATACAAAGAACCAGAAAGACCAGTGCAGCATGAGGAGTCAATA
GAGGGAGAAGCTGACCACAGTGGCTATGCCGGAGAGGTGGGCTTCCGTGCCTTCTCTGGTTCTGGGAATA
GACTGGATGGGAAGAAAAAAGGGGTTGAGCCCAGTCCCTCCCCAATCAAGCCTGGAGACATCAAAAGAGG
AATTCCTAATTACGAATTTAAGCTTGGTAAGATCACTTTCATCAGAAATTCACGTCCATTGGTCAAAAAG
GTTGAAGAGGATGAAGCTGGAGGCAGATTCATTGCTTTCTCTGGAGAAGGACAGTCACTGCGTAAGAAGG
GAAGAAAGCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204334 protein sequence
Red=Cloning site Green=Tags(s)

MFSFNMFDHPIPRVFQNRFSTQYRCFSVSMLAGPNDRSDVEKGGKIIMPPSALDQLSRLNITYPMLFKLT
NKNSDRMTHCGVLEFVADEGICYLPHWMMQNLLLEEGGLVQVESVNLQVATYSKFQPQSPDFLDITNPKA
VLENALRNFACLTTGDVIAINYNEKIYELRVMETKPDKAVSIIECDMNVDFDAPLGYKEPERPVQHEESI
EGEADHSGYAGEVGFRAFSGSGNRLDGKKKGVEPSPSPIKPGDIKRGIPNYEFKLGKITFIRNSRPLVKK
VEEDEAGGRFIAFSGEGQSLRKKGRKP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011672
ORF Size 921 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011672.4, NP_035802.3
RefSeq Size 1996 bp
RefSeq ORF 924 bp
Locus ID 22230
UniProt ID P70362
Cytogenetics 16 11.65 cM
MW 34.5 kDa
Summary Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and NPLOC4, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I (By similarity).UniProtKB/Swiss-Prot Function
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"NM_011672" in other vectors (4)
SKU Description Size Price
MC208050 Ufd1l (untagged) - Mouse ubiquitin fusion degradation 1 like (Ufd1l), transcript variant 1, (10ug) 10 ug
$330.00
MG204334 Ufd1l (tGFP-tagged) - Mouse ubiquitin fusion degradation 1 like (Ufd1l) 10 ug
$500.00
MR204334L3 Lenti ORF clone of Ufd1l (Myc-DDK-tagged) - Mouse ubiquitin fusion degradation 1 like (Ufd1l), transcript variant 1 10 ug
$600.00
MR204334L4 Lenti ORF clone of Ufd1l (mGFP-tagged) - Mouse ubiquitin fusion degradation 1 like (Ufd1l), transcript variant 1 10 ug
$600.00

Plasmid Map for MR204334

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204334
Circular map for MR204334

file-alt Citations

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