OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Rsph1 (NM_025290) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR204219
Rsph1 (Myc-DDK-tagged) - Mouse radial spoke head 1 homolog (Chlamydomonas) (Rsph1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Rsph1 or NM_025290"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rsph1
Synonyms MCA; Tsga2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204219 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGACCTGGGCTCTGAGGAGTTAGAGGAGGAGGGAGAGAACGACCTTGGGGAGTACGAAGGGGAGC
GCAATGAGGTGGGAGAACGACACGGACATGGGAAAGCACGACTGCCCAACGGGGACACATATGAAGGAAG
CTATGAGTTTGGAAAAAGACATGGCCAGGGGACTTACAAATTTAAGAATGGGGCCCGGTACACCGGAGAC
TATGTCAAAAATAAAAAGCACGGCCAAGGCACCTTTATCTATCCAGATGGATCCAGATATGAAGGGGAGT
GGGCTGATGACCAGAGGCACGGCCAAGGCGTGTACTACTATGTCAACAATGACACCTACACAGGGGAGTG
GTTCAATCATCAAAGGCACGGGCAAGGCACCTACCTCTACGCAGAGACCGGCAGTAAGTATGTCGGTACC
TGGGTGCACGGACAGCAGGAGGGTGCTGCCGAGCTCATCCACCTAAACCACAGGTACCAGGGCAAGTTCA
TGAACAAAAATCCTGTGGGCCCTGGAAAGTACGTATTTGATATTGGATGCGAACAGCACGGTGAATATCG
CCTCACAGATACGGAAAGAGGAGAAGAGGAGGAGGAGGAAGAGACATTAGTGAACATCGTTCCAAAATGG
AAAGCTCTCAACATCACAGAATTGGCCCTGTGGACTCCAACCCTCTCCGAGGAGCAGCCTCCCCCTGAGG
GCCAAGGCCAGGAGGAACCGCAGGGACTTACTGGCGTGGGTGACCCCTCAGAAGACATCCAGGCAGAAGG
TTTTGAGGGCGAGCTGGAGCCGAGGGGGGCCGATGAAGACGTAGACACGTTCAGGCAGGAGAGCCAGGAG
AACAGCTACGACATAGACCAGGGAAACTTGAACTTTGATGAAGAACCGTCAGACCTCCAGGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204219 protein sequence
Red=Cloning site Green=Tags(s)

MSDLGSEELEEEGENDLGEYEGERNEVGERHGHGKARLPNGDTYEGSYEFGKRHGQGTYKFKNGARYTGD
YVKNKKHGQGTFIYPDGSRYEGEWADDQRHGQGVYYYVNNDTYTGEWFNHQRHGQGTYLYAETGSKYVGT
WVHGQQEGAAELIHLNHRYQGKFMNKNPVGPGKYVFDIGCEQHGEYRLTDTERGEEEEEEETLVNIVPKW
KALNITELALWTPTLSEEQPPPEGQGQEEPQGLTGVGDPSEDIQAEGFEGELEPRGADEDVDTFRQESQE
NSYDIDQGNLNFDEEPSDLQD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025290
ORF Size 903 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025290.4
RefSeq Size 1164 bp
RefSeq ORF 906 bp
Locus ID 22092
UniProt ID Q8VIG3
Cytogenetics 17 15.94 cM
MW 34.2 kDa
Summary The specific expression during male germ cell development and its characteristic localization suggest that it may play an important role in male meiosis. It is necessary for proper building of the axonemal central pair and radial spokes (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_025290" in other vectors (4)
SKU Description Size Price
MC209592 Rsph1 (untagged) - Mouse radial spoke head 1 homolog (Chlamydomonas) (Rsph1), (10ug) 10 ug
$330.00
MG204219 Rsph1 (tGFP-tagged) - Mouse testis specific gene A2 (Tsga2) 10 ug
$500.00
MR204219L3 Lenti ORF clone of Rsph1 (Myc-DDK-tagged) - Mouse radial spoke head 1 homolog (Chlamydomonas) (Rsph1) 10 ug
$600.00
MR204219L4 Lenti ORF clone of Rsph1 (mGFP-tagged) - Mouse radial spoke head 1 homolog (Chlamydomonas) (Rsph1) 10 ug
$600.00

Plasmid Map for MR204219

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204219
Circular map for MR204219

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products