Clk4 (BC002220) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204207
Clk4 (Myc-DDK-tagged) - Mouse CDC like kinase 4 (cDNA clone MGC:7477 IMAGE:3490616)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Clk4
Synonyms AI987988; C85119
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204207 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGGCTTACATGTAGCAGTGAAAATTGTAAAAAATGTAGGCCGTTACCGGGAGGCAGCTCGTTCTG
AAATCCAAGTATTGGAGCACTTGAACAGCACTGACCCCAACAGTGTCTTCCGATGCGTCCAGATGCTAGA
GTGGTTTGATCATCATGGTCATGTTTGTATTGTGTTTGAGCTGCTGGGACTTAGTACCTATGATTTTATT
AAAGAAAATAGTTTTCTGCCATTTCAAATTGATCACATCAGGCAAATGGCTTATCAGATCTGCCAGTCTA
TAAATTTTTTACATCATAATAAATTAACACACACGGACCTAAAACCTGAAAATATTTTATTTGTGAAGTC
TGACTATGTAGTCAAATACAATTCTAAAATGAAACGAGATGAGCGCACATTGAAAAACACAGATATCAAA
GTTGTTGATTTTGGAAGTGCAACATATGACGACGAACATCATAGTACTTTGGTGTCCACAAGGCACTACA
GGGCTCCAGAGGTCATTTTGGCTCTAGGTTGGTCTCAGCCTTGTGATGTTTGGAGCATAGGCTGCATTCT
TATTGAGTACTACCTTGGGTTCACAGTCTTTCAGACCCACGATAGTAAAGAGCACCTGGCAATGATGGAG
CGGATCTTAGGACCCATCCCAGCACATATGATCCAGAAGACAAGGAAACGCAAGTATTTCCACCATAACC
AGCTAGATTGGGACGAGCATAGTTCAGCTGGGAGATATGTTAGGAGACGCTGCAAGCCGTTAAAGGAATT
TATGCTGTGTCATGACGAAGAGCATGAGAAGCTGTTTGACCTGGTTCGAAGAATGTTGGAGTATGACCCA
GCGAGAAGGATCACCTTGGATGAAGCATTGCAGCACCCTTTCTTTGACTTATTAAAAAGGAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204207 protein sequence
Red=Cloning site Green=Tags(s)

MDGLHVAVKIVKNVGRYREAARSEIQVLEHLNSTDPNSVFRCVQMLEWFDHHGHVCIVFELLGLSTYDFI
KENSFLPFQIDHIRQMAYQICQSINFLHHNKLTHTDLKPENILFVKSDYVVKYNSKMKRDERTLKNTDIK
VVDFGSATYDDEHHSTLVSTRHYRAPEVILALGWSQPCDVWSIGCILIEYYLGFTVFQTHDSKEHLAMME
RILGPIPAHMIQKTRKRKYFHHNQLDWDEHSSAGRYVRRRCKPLKEFMLCHDEEHEKLFDLVRRMLEYDP
ARRITLDEALQHPFFDLLKRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC002220
ORF Size 903 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC002220, AAH02220
RefSeq Size 1445 bp
RefSeq ORF 905 bp
Locus ID 12750
Cytogenetics 11 B1.3
MW 35.8 kDa
Summary Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates SRSF1 and SRSF3. Required for the regulation of alternative splicing of MAPT/TAU. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC002220" in other vectors (3)
SKU Description Size Price
MG204207 Clk4 (tGFP-tagged) - Mouse CDC like kinase 4 (cDNA clone MGC:7477 IMAGE:3490616) 10 ug
$500.00
MR204207L3 Lenti ORF clone of Clk4 (Myc-DDK-tagged) - Mouse CDC like kinase 4 (cDNA clone MGC:7477 IMAGE:3490616) 10 ug
$600.00
MR204207L4 Lenti ORF clone of Clk4 (mGFP-tagged) - Mouse CDC like kinase 4 (cDNA clone MGC:7477 IMAGE:3490616) 10 ug
$600.00

Plasmid Map for MR204207

Download sequence for

Cloning scheme for MR204207

Circular map for MR204207

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