Bphl (NM_026512) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR203984
Bphl (Myc-DDK-tagged) - Mouse biphenyl hydrolase-like (serine hydrolase, breast epithelial mucin-associated antigen) (Bphl)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bphl
Synonyms 2010012D11Rik; 5730533B08Rik; AI115341
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203984 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACGGCAACAGTTCGCCCGGCGGCCCAGCGGTTGCGGCTGCTGCTCTCGCCCCTGAAATCCCGGA
TCTGCGTACCCCAGGCCGAACCCGTAGCCACCTTCGGCACTGCAGTAACTTCTGCCAAGGTGGCTGTGAA
TGGCGTTCACCTGCATTACCAGCGCGTGGGAGAAGGGGAACATGTGATCCTCCTGCTTCCTGGGATGTTG
GGTAGCGGAAAGACCGATTTTGCACCTCAGCTTCAGAGCCTAAATAAGAAGCGCTTCACATTGGTGGCCT
GGGACCCTCGAGGATATGGATATTCCAGACCCCCAGATCGAGATTTTCCACGGGATTTTTTTGAAAGGGA
TGCAAAGGATGCTGTTGACTTGATGAAGGCTCTACAGTTCAAGCAGGTCTCCCTCCTGGGCTGGAGTGAT
GGCGGCATAACTGCACTCATCGCTGCTGCAAAGTACCCATCTTATATCCGCAAGATGGTGATCTGGGGAG
CAAATGCCTATGTCACCGAGGAAGACAGCAGGATTTACCAGGGTATCCGAGATGTTTCTAAATGGAGTGA
GAAGGCAAGGAAACCCCTGGAAGCCCTGTATGGATATGACTACCTTGCCAAAACCTGTGAGGACTGGGTG
GATGGCATAAGCCAGTTTAAACAACTGCCAGAGGGTAACATCTGCCGGCACTTGCTGCCCCTGGTCCAGT
GCCCTACCCTCATTGTGCATGGGGAGAAGGACCCATTGGTCCCCCGCTTTCATGCTGACTTCCTTCTCCA
GCATGTGAAAGGGTCACGGTTGCATCTGATGCCAGAAGGCAAGCACAACTTACACTTACGGTTTGCAGAC
GAATTCAACAGGTTGGTAGAAGACTTTCTACAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR203984 protein sequence
Red=Cloning site Green=Tags(s)

MATATVRPAAQRLRLLLSPLKSRICVPQAEPVATFGTAVTSAKVAVNGVHLHYQRVGEGEHVILLLPGML
GSGKTDFAPQLQSLNKKRFTLVAWDPRGYGYSRPPDRDFPRDFFERDAKDAVDLMKALQFKQVSLLGWSD
GGITALIAAAKYPSYIRKMVIWGANAYVTEEDSRIYQGIRDVSKWSEKARKPLEALYGYDYLAKTCEDWV
DGISQFKQLPEGNICRHLLPLVQCPTLIVHGEKDPLVPRFHADFLLQHVKGSRLHLMPEGKHNLHLRFAD
EFNRLVEDFLQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026512
ORF Size 873 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026512.1, NP_080788.1
RefSeq Size 1224 bp
RefSeq ORF 876 bp
Locus ID 68021
UniProt ID Q8R164
Cytogenetics 13 A3.3
MW 32.9 kDa
Summary Serine hydrolase that catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. Activates valacyclovir to acyclovir. May play a role in detoxification processes. It is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026512" in other vectors (4)
SKU Description Size Price
MC202243 Bphl (untagged) - Mouse biphenyl hydrolase-like (serine hydrolase, breast epithelial mucin-associated antigen) (Bphl), (10ug) 10 ug
$300.00
MG203984 Bphl (tGFP-tagged) - Mouse biphenyl hydrolase-like (serine hydrolase, breast epithelial mucin-associated antigen) (Bphl) 10 ug
$500.00
MR203984L3 Lenti ORF clone of Bphl (Myc-DDK-tagged) - Mouse biphenyl hydrolase-like (serine hydrolase, breast epithelial mucin-associated antigen) (Bphl) 10 ug
$600.00
MR203984L4 Lenti ORF clone of Bphl (mGFP-tagged) - Mouse biphenyl hydrolase-like (serine hydrolase, breast epithelial mucin-associated antigen) (Bphl) 10 ug
$600.00

Plasmid Map for MR203984

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Cloning scheme for MR203984

Circular map for MR203984

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