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Mlec (NM_175403) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203976
Mlec (Myc-DDK-tagged) - Mouse malectin (Mlec)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mlec
Synonyms 2410014A08Rik; AI850530; ESTM19; mKIAA0152; X83352
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203976 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGAGGCCCCGGGGAGCCGAGGGAACCGCTGTGGCGCTTCTCCGGCTGCTTCTGTTGCTGCTGCTGC
TGGGACCGAAGCTCCGGGGTCCGGGGCTCGGCGTGGTCGGCGCCGCCGGGGCCGGACTGCCGGAGAGCGT
CATCTGGGCAGTGAACGCAGGCGGCGAGGCGCATGTGGACGTGCACGGGATCCACTTCCGCAAGGACCCT
CTGGAAGGCCGGGTGGGCCGAGCCTCGGACTATGGCATGAAACTGCCGATCCTGCGCTCCACCCCGGAGG
ACCAGATCCTATATCAGACAGAGCGGTACAATGAAGAGACCTTTGGCTACGAAGTACCCGTCAAGGAGGA
GGGCGACTATGTGCTGGTGTTGAAGTTTGCTGAGGTCTACTTTGCACAGTCTCAGCAGAAGGTGTTTGAT
GTCCGGTTGAATGGCCATGTGGTGGTGAAGGACTTGGACATCTTTGACCGTGTCGGGCACAGCACGGCCC
ATGATGAAATCATCCCGATGAGCATCAGGAAGGGGAAGCTGAGTGTGCGGGGGGAGGTGTCCACCTTCAC
AGGGAAGCTATACATCGAGTTTGTCAAGGGGTACTATGACAATCCCAAAGTCTGTGCACTCTACATCCTG
GCTGGGACAGTAGACGATGTACCAAAGCTGCAGCCTCATCCAGGATTGGAAAAGAAAGAAGAGGAGGAGG
AGGAGGAGGAATATGATGAGGGGTCTAATCTCAAAAGACAGACCAACAAGAACAGGGTGCAGTCAGGCCC
CCGCACGCCTAACCCCTATGCCTCGGACAACAGCAGCCTCATGTTCCCTATCCTGGTGGCCTTCGGAGTG
TTCATTCCAACCCTCTTCTGCCTCTGCCGCTTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203976 protein sequence
Red=Cloning site Green=Tags(s)

MLRPRGAEGTAVALLRLLLLLLLLGPKLRGPGLGVVGAAGAGLPESVIWAVNAGGEAHVDVHGIHFRKDP
LEGRVGRASDYGMKLPILRSTPEDQILYQTERYNEETFGYEVPVKEEGDYVLVLKFAEVYFAQSQQKVFD
VRLNGHVVVKDLDIFDRVGHSTAHDEIIPMSIRKGKLSVRGEVSTFTGKLYIEFVKGYYDNPKVCALYIL
AGTVDDVPKLQPHPGLEKKEEEEEEEEYDEGSNLKRQTNKNRVQSGPRTPNPYASDNSSLMFPILVAFGV
FIPTLFCLCRL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175403
ORF Size 873 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175403.3
RefSeq Size 5970 bp
RefSeq ORF 876 bp
Locus ID 109154
UniProt ID Q6ZQI3
Cytogenetics 5 55.99 cM
MW 32.3 kDa
Summary Carbohydrate-binding protein with a strong ligand preference for Glc2-N-glycan. May play a role in the early steps of protein N-glycosylation (By similarity).UniProtKB/Swiss-Prot Function
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"NM_175403" in other vectors (4)
SKU Description Size Price
MC204835 Mlec (untagged) - Mouse malectin (Mlec), (10ug) 10 ug
$300.00
MG203976 Mlec (tGFP-tagged) - Mouse RIKEN cDNA 2410014A08 gene (2410014A08Rik) 10 ug
$500.00
MR203976L3 Lenti ORF clone of Mlec (Myc-DDK-tagged) - Mouse malectin (Mlec) 10 ug
$600.00
MR203976L4 Lenti ORF clone of Mlec (mGFP-tagged) - Mouse malectin (Mlec) 10 ug
$600.00

Plasmid Map for MR203976

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203976
Circular map for MR203976

file-alt Citations

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