Mff (NM_029409) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR203974
Mff (Myc-DDK-tagged) - Mouse mitochondrial fission factor (Mff), nuclear gene encoding mitochondrial protein
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mff
Synonyms 5230400G24Rik; AI314724
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203974 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAAATTAGTCGAATTCAGTATGAAATGGAATACACCGAAGGTATTAGTCAGCGAATGAGGGTCC
CGGAAAAATTAAAAGTAGCACCACCAAATGCTGACCTGGAGCAAGAGTTCCAAGATGGAGTTCCAAATGC
CAGTGTGATAATGCAAGTCCCAGAGAGGATCGTGGTTACAGGAAATAATGAAGACATTTCATTTTCAAGA
CCAGCAGATCTTGACCTTATCCAGTCCACTCCCTTTAAACCTCTGGCACTGAAAACACCACCACGCGTGC
TCACACTGAGTGAGAGACCACTAGATTTCCTGGATTTAGAAAGACCACTTCCAACTCCTCAAAGCGAAGA
GAGCCGAGCAGTTGGCAGGCTAAAAAGAGAGCGTTCTATGAGTGAAAATGCTGTTCGCCAAAATGGACAG
TTGGTCAGAAACGATTCCATTGTGACACCATCACCACCACAGGCTCGGGTCTGTCCTCCCCATATGCTAC
CTGAAGATGGAGCTAATCTTTCCTCTGCCCGTGGCATTTTGTCGCTTATCCAGTCTTCTACTCGTAGGGC
TTACCAGCAGATCTTGGATGTGCTGGATGAAAACCGCAGACCTGTGTTGCGTGGTGGGTCAGCTGCCGCC
ACTTCTAATCCTCATCATGACAACGTCAGGTATGGCATTTCAAACCTAGATGCAGCCATTGAAGGAGCTT
CAGATGACATGACTGTGGTAGATGCAGCTTCATTAAGACGTCAGATAATCAAATTAAATAGACGTCTCCA
ACTTCTGGAAGAGGAGAATAAAGAGCGTGCTAAAAGAGAAATGGTCATGTATTCAATCACTGTAGCGTTC
TGGCTGCTTAACAGCTGGCTCTGGTTTCGACGC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR203974 protein sequence
Red=Cloning site Green=Tags(s)

MAEISRIQYEMEYTEGISQRMRVPEKLKVAPPNADLEQEFQDGVPNASVIMQVPERIVVTGNNEDISFSR
PADLDLIQSTPFKPLALKTPPRVLTLSERPLDFLDLERPLPTPQSEESRAVGRLKRERSMSENAVRQNGQ
LVRNDSIVTPSPPQARVCPPHMLPEDGANLSSARGILSLIQSSTRRAYQQILDVLDENRRPVLRGGSAAA
TSNPHHDNVRYGISNLDAAIEGASDDMTVVDAASLRRQIIKLNRRLQLLEEENKERAKREMVMYSITVAF
WLLNSWLWFRR

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_029409
ORF Size 873 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029409.3, NP_083685.2
RefSeq Size 1920 bp
RefSeq ORF 876 bp
Locus ID 75734
UniProt ID Q6PCP5
Cytogenetics 1 C5
MW 32.9 kDa
Summary Plays a role in mitochondrial and peroxisomal fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. May be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles.UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_029409" in other vectors (6)
SKU Description Size Price
MC207757 Mff (untagged) - Mouse mitochondrial fission factor (Mff), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$330.00
MG203974 Mff (tGFP-tagged) - Mouse RIKEN cDNA 5230400G24 gene (5230400G24Rik) 10 ug
$650.00
MR203974L1 Lenti ORF clone of Mff (Myc-DDK-tagged) - Mouse mitochondrial fission factor (Mff), nuclear gene encoding mitochondrial protein 10 ug
$750.00
MR203974L2 Lenti ORF clone of Mff (mGFP-tagged) - Mouse mitochondrial fission factor (Mff), nuclear gene encoding mitochondrial protein 10 ug
$750.00
MR203974L3 Lenti ORF clone of Mff (Myc-DDK-tagged) - Mouse mitochondrial fission factor (Mff), nuclear gene encoding mitochondrial protein 10 ug
$750.00
MR203974L4 Lenti ORF clone of Mff (mGFP-tagged) - Mouse mitochondrial fission factor (Mff), nuclear gene encoding mitochondrial protein 10 ug
$750.00

Plasmid Map for MR203974

Download sequence for

Cloning scheme for MR203974

Circular map for MR203974

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products