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Tia1 (BC023813) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203884
Tia1 (Myc-DDK-tagged) - Mouse cytotoxic granule-associated RNA binding protein 1 (cDNA clone MGC:36034 IMAGE:5353938)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tia1
Synonyms 2310050N03Rik; AI256674; mTIA-1; TIA-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203884 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGACGAGATGCCCAAGACTCTATACGTCGGTAACCTTTCTCGAGATGTGACAGAAGCTCTCATCC
TCCAGCTCTTTAGCCAGATTGGACCTTGTAAAAACTGCAAAATGATTATGGATACAGCAGGAAATGACCC
ATACTGTTTTGTGGAGTTCCATGAGCACCGTCATGCAGCTGCAGCCCTGGCTGCCATGAATGGGCGGAAG
ATAATGGGTAAGGAAGTGAAAGTGAATTGGGCAACAACCCCTAGCAGTCAAAAGAAGGATACGAGCAATC
ATTTCCATGTGTTTGTTGGTGACCTCAGTCCAGAAATCACAACCGAAGACATCAAAGCAGCGTTTGCACC
ATTTGGAAGAATTTCAGATGCCCGTGTGGTAAAAGACATGGCTACCGGGAAGTCTAAGGGATATGGCTTT
GTCTCCTTTTTCAACAAATGGGATGCAGAAAATGCCATTCAGCAGATGGGTGGCCAGTGGCTTGGTGGAA
GACAAATCAGAACTAACTGGGCAACCCGAAAGCCTCCAGCTCCAAAGAGTACATATGAGTCGAACACCAA
ACAGCTGTCATATGATGAGGTTGTGAGTCAGTCTAGTCCCAACAACTGCACTGTGTACTGTGGAGGGGTG
ACGTCAGGACTGACAGAACAACTAATGCGTCAGACTTTTTCTCCATTTGGACAAATAATGGAAATTCGAG
TCTTCCCAGATAAAGGATACTCATTTGTTCGTCAGACCCAGTCTTCTGCACCGTGGATGGGACCCAATTA
CAGTGTGCCACCACCTCAAGGGCAGAATGGCAGCATGTTGCCTAGTCAGCCTGCTGGGTATCGAGTGGCC
GGGTATGAAACCCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203884 protein sequence
Red=Cloning site Green=Tags(s)

MEDEMPKTLYVGNLSRDVTEALILQLFSQIGPCKNCKMIMDTAGNDPYCFVEFHEHRHAAAALAAMNGRK
IMGKEVKVNWATTPSSQKKDTSNHFHVFVGDLSPEITTEDIKAAFAPFGRISDARVVKDMATGKSKGYGF
VSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYESNTKQLSYDEVVSQSSPNNCTVYCGGV
TSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFVRQTQSSAPWMGPNYSVPPPQGQNGSMLPSQPAGYRVA
GYETQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC023813
ORF Size 855 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC023813, AAH23813
RefSeq Size 1455 bp
RefSeq ORF 857 bp
Locus ID 21841
Cytogenetics 6 D1
MW 31.6 kDa
Summary Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis (By similarity).UniProtKB/Swiss-Prot Function
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"BC023813" in other vectors (4)
SKU Description Size Price
MC217759 Tia1 (untagged) - Mouse cytotoxic granule-associated RNA binding protein 1 (cDNA clone MGC:36034 IMAGE:5353938), (10ug) 10 ug
$330.00
MG203884 Tia1 (tGFP-tagged) - Mouse cytotoxic granule-associated RNA binding protein 1 (cDNA clone MGC:36034 IMAGE:5353938) 10 ug
$500.00
MR203884L3 Lenti ORF clone of Tia1 (Myc-DDK-tagged) - Mouse cytotoxic granule-associated RNA binding protein 1 (cDNA clone MGC:36034 IMAGE:5353938) 10 ug
$600.00
MR203884L4 Lenti ORF clone of Tia1 (mGFP-tagged) - Mouse cytotoxic granule-associated RNA binding protein 1 (cDNA clone MGC:36034 IMAGE:5353938) 10 ug
$600.00

Plasmid Map for MR203884

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203884
Circular map for MR203884

file-alt Citations

YB-1 regulates stress granule formation and tumor progression by translationally activating G3BP1
Somasekharan, El-Naggar, Leprivier et al
J. Cell Biol. (2015) 208 (7), 913-29 DOI: 10.1083/jcb.201411047
Cited by 290
AI Summary: In this study, OriGene’s Myc-DDK–tagged G3BP1 and Myc-DDK–tagged TIA-1 plasmids were used to investigate the roles of these proteins in stress granule formation and tumor progression.
Snippet: …TranscriptAid T7 High Yield Transcription kit, RNA 3′ End Biotinylation kit, LightShift Chemiluminescent RNA EMSA kit, and Zeba Spin desalting columns (7K MWCO, 2 mL) were from Thermo Fisher Scientific. Myc-DDK–tagged G3BP1 (CMV promoter) and Myc-DDK-tagged TIA-1 (CMV promoter) were from OriGene. 5-FAM-oligodT probe was from Integrated DNA Technologies. …
Showing 1-1 of 1 papers.
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