Miox (NM_019977) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR203880
Miox (Myc-DDK-tagged) - Mouse myo-inositol oxygenase (Miox)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Miox
Synonyms 0610009I10Rik; AI314022; Aldrl6; C85427; RSOR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203880 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGTCGATGTGGGCCCAGACCCTTCCCTGGTCTATCGACCCGATGTGGACCCAGAGATGGCCAAAA
GCAAGGACAGCTTCCGAAACTATACTTCAGGCCCGCTGCTGGATCGTGTCTTTACCACATACAAGCTCAT
GCACACTCACCAGACTGTGGACTTCGTCAGCAGGAAGCGCATCCAGTATGGAAGCTTCTCTTACAAGAAG
ATGACCATCATGGAGGCTGTGGGCATGCTGGATGATCTGGTGGACGAATCTGACCCAGACGTAGATTTCC
CCAACTCCTTCCACGCGTTCCAGACCGCGGAGGGCATCCGGAAAGCCCACCCGGACAAGGACTGGTTCCA
CCTGGTCGGACTTTTGCACGATCTGGGGAAAATTATGGCTCTGTGGGGGGAACCTCAGTGGGCTGTTGTT
GGAGACACGTTCCCCGTGGGCTGCCGTCCCCAGGCCTCTGTGGTGTTCTGTGACTCTACTTTCCAGGACA
ATCCTGACCTCCAGGATCCTCGATACAGCACAGAACTCGGCATGTACCAGCCTCACTGTGGACTAGAGAA
CGTCCTTATGTCCTGGGGCCATGATGAGTACCTATACCAGATGATGAAGTTCAACAAGTTCTCCCTGCCT
TCAGAGGCCTTCTACATGATCCGATTCCACTCCTTCTATCCGTGGCACACCGGCGGTGACTACCGGCAGC
TGTGCAGCCAGCAGGACCTGGATATGCTGCCCTGGGTGCAAGAGTTCAACAAGTTTGATCTCTACACGAA
GTGCCCTGACCTACCGGATGTGGAGAGCCTGCGGCCCTACTATCAAGGGCTGATTGACAAGTACTGCCCG
GGCACCCTGAGCTGG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR203880 protein sequence
Red=Cloning site Green=Tags(s)

MKVDVGPDPSLVYRPDVDPEMAKSKDSFRNYTSGPLLDRVFTTYKLMHTHQTVDFVSRKRIQYGSFSYKK
MTIMEAVGMLDDLVDESDPDVDFPNSFHAFQTAEGIRKAHPDKDWFHLVGLLHDLGKIMALWGEPQWAVV
GDTFPVGCRPQASVVFCDSTFQDNPDLQDPRYSTELGMYQPHCGLENVLMSWGHDEYLYQMMKFNKFSLP
SEAFYMIRFHSFYPWHTGGDYRQLCSQQDLDMLPWVQEFNKFDLYTKCPDLPDVESLRPYYQGLIDKYCP
GTLSW

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_019977
ORF Size 855 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019977.2, NP_064361.2
RefSeq Size 1074 bp
RefSeq ORF 858 bp
Locus ID 56727
UniProt ID Q9QXN5
Cytogenetics 15 E3
MW 33.2 kDa
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Documents
Resources
"NM_019977" in other vectors (4)
SKU Description Size Price
MC201149 Miox (untagged) - Mouse myo-inositol oxygenase (Miox), (10ug) 10 ug
$300.00
MG203880 Miox (tGFP-tagged) - Mouse myo-inositol oxygenase (Miox) 10 ug
$500.00
MR203880L3 Lenti ORF clone of Miox (Myc-DDK-tagged) - Mouse myo-inositol oxygenase (Miox) 10 ug
$600.00
MR203880L4 Lenti ORF clone of Miox (mGFP-tagged) - Mouse myo-inositol oxygenase (Miox) 10 ug
$600.00

Plasmid Map for MR203880

Download sequence for

Cloning scheme for MR203880

Circular map for MR203880

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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