Xpa (BC029241) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR203762
Xpa (Myc-DDK-tagged) - Mouse xeroderma pigmentosum, complementation group A (cDNA clone MGC:36016 IMAGE:4489063)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Xpa
Synonyms AI573865; Xpac
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203762 representing BC029241
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACGGCCGAGGAAAAGCAGACGTCACCTGAGCCCGTGGCCGCGGACGAGCCCGCGCAGCTGCCGG
CCGCCGTACGCGCGAGCGTGGAGCGGAAGCGACAGCGGGCACTGATGCTGCGCCAGGCTCGGCTGGCTGC
GCGGCCCTACCCCGCGGCGGCGGCCACCGGAGGCGTGGCCAGTGTAAAAGCAGCTCCCAAAATGATTGAC
ACCAAAGGTGGCTTCATTTTAGAAGAGGAAGAAGAGAAACACGAGATTGGAAACATTGTTCATGAACCAG
GGCCCGTCATGGAGTTTGATTACACCATCTGTGAAGAATGCGGGAAAGAGTTCATGGACTCGTACCTTAT
GAACCACTTTGATCTGCCAACGTGTGACAGCTGCAGAGATGCTGATGATAAACACAAGCTTATAACCAAG
ACAGAAGCGAAGCAAGAGTACCTTCTGAAGGACTGTGATCTAGAGAAGAGAGAGCCAGCGCTCAGGTTCC
TTGTGAAGAAGAACCCACGCCATTCACAGTGGGGCGATATGAAGCTCTACCTAAAGCTACAGGTGGTAAA
GCGGGCTCTTGAAGTGTGGGGTAGCCAGGAAGCTCTGGAAGATGCAAAGGAAGTGAGGCAGGAAAACCGA
GAAAAAATGAAGCAGAAGAAGTTTGATAAAAAAGTAAAAGTAGGACTTGATGATGCAGTGAAGATTCTCC
TCGATGACAAACCTCTCCACCGAATGGCTCCCTGGCATAACAGGGCCCTGGAAAAAAGATCAAGTGTACT
CACACGCACAACCACAAAACAGATTTCTTTCATTTATTCAATAAACACTTGCGACTGTTGTGCTAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR203762 representing BC029241
Red=Cloning site Green=Tags(s)

MATAEEKQTSPEPVAADEPAQLPAAVRASVERKRQRALMLRQARLAARPYPAAAATGGVASVKAAPKMID
TKGGFILEEEEEKHEIGNIVHEPGPVMEFDYTICEECGKEFMDSYLMNHFDLPTCDSCRDADDKHKLITK
TEAKQEYLLKDCDLEKREPALRFLVKKNPRHSQWGDMKLYLKLQVVKRALEVWGSQEALEDAKEVRQENR
EKMKQKKFDKKVKVGLDDAVKILLDDKPLHRMAPWHNRALEKRSSVLTRTTTKQISFIYSINTCDCCAK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC029241
ORF Size 837 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC029241.1
RefSeq Size 1234 bp
RefSeq ORF 839 bp
Locus ID 22590
Cytogenetics 4 24.49 cM
MW 45.2 kDa
Summary Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC029241" in other vectors (4)
SKU Description Size Price
MC218270 Xpa (untagged) - Mouse xeroderma pigmentosum, complementation group A (cDNA clone MGC:36016 IMAGE:4489063), (10ug) 10 ug
$330.00
MG203762 Xpa (tGFP-tagged) - Mouse xeroderma pigmentosum, complementation group A (cDNA clone MGC:36016 IMAGE:4489063) 10 ug
$500.00
MR203762L3 Lenti ORF clone of Xpa (Myc-DDK-tagged) - Mouse xeroderma pigmentosum, complementation group A (cDNA clone MGC:36016 IMAGE:4489063) 10 ug
$600.00
MR203762L4 Lenti ORF clone of Xpa (mGFP-tagged) - Mouse xeroderma pigmentosum, complementation group A (cDNA clone MGC:36016 IMAGE:4489063) 10 ug
$600.00

Plasmid Map for MR203762

Download sequence for

Cloning scheme for MR203762

Circular map for MR203762

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