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Tpsb2 (NM_010781) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203701
Tpsb2 (Myc-DDK-tagged) - Mouse tryptase beta 2 (Tpsb2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tpsb2
Synonyms AV011504; Mcp-6; Mcp6; Mcpt6; MMCP-6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203701 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGAAGCGGCGGCTGCTGCTGCTGTGGGCACTGTCCCTCCTGGCTAGTCTGGTGTACTCAGCCCCTC
GCCCAGCCAATCAGCGAGTGGGCATCGTGGGAGGACATGAGGCTTCTGAGAGTAAGTGGCCCTGGCAGGT
GAGCCTGAGATTTAAATTAAACTACTGGATACATTTCTGCGGAGGCTCTCTCATCCACCCACAGTGGGTG
CTCACTGCGGCACACTGTGTGGGACCGCACATCAAAAGCCCACAGCTCTTCCGGGTGCAGCTTCGTGAGC
AGTATCTATACTATGGGGACCAGCTCCTCTCTTTGAACCGGATCGTGGTGCACCCCCACTATTACACGGC
CGAGGGTGGGGCAGACGTTGCCCTGCTGGAGCTTGAGGTCCCTGTGAATGTCTCCACCCATATCCACCCC
ATATCCCTGCCCCCTGCCTCGGAGACCTTCCCCCCTGGGACATCGTGCTGGGTGACAGGCTGGGGCGACA
TTGATAATGACGAGCCTCTCCCACCTCCTTATCCTCTGAAGCAAGTGAAGGTTCCCATTGTGGAAAACAG
CCTGTGTGACCGGAAGTACCACACTGGCCTCTACACGGGAGATGATTTTCCCATTGTCCATGATGGCATG
CTGTGTGCTGGAAATACCAGGAGAGACTCCTGCCAGGGCGATTCAGGGGGGCCACTGGTCTGCAAAGTGA
AGGGTACCTGGCTGCAGGCAGGAGTGGTCAGCTGGGGTGAGGGCTGCGCACAGCCCAACAAGCCTGGCAT
CTACACCCGGGTGACATACTACTTAGACTGGATCCACCGCTATGTCCCTGAGCATTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203701 protein sequence
Red=Cloning site Green=Tags(s)

MLKRRLLLLWALSLLASLVYSAPRPANQRVGIVGGHEASESKWPWQVSLRFKLNYWIHFCGGSLIHPQWV
LTAAHCVGPHIKSPQLFRVQLREQYLYYGDQLLSLNRIVVHPHYYTAEGGADVALLELEVPVNVSTHIHP
ISLPPASETFPPGTSCWVTGWGDIDNDEPLPPPYPLKQVKVPIVENSLCDRKYHTGLYTGDDFPIVHDGM
LCAGNTRRDSCQGDSGGPLVCKVKGTWLQAGVVSWGEGCAQPNKPGIYTRVTYYLDWIHRYVPEHS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010781
ORF Size 828 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010781.2
RefSeq Size 1088 bp
RefSeq ORF 831 bp
Locus ID 17229
Cytogenetics 17 12.53 cM
MW 30.9 kDa
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"NM_010781" in other vectors (4)
SKU Description Size Price
MC202130 Tpsb2 (untagged) - Mouse tryptase beta 2 (Tpsb2), (10ug) 10 ug
$300.00
MG203701 Tpsb2 (tGFP-tagged) - Mouse mast cell protease 6 (Mcpt6) 10 ug
$500.00
MR203701L3 Lenti ORF clone of Tpsb2 (Myc-DDK-tagged) - Mouse tryptase beta 2 (Tpsb2) 10 ug
$600.00
MR203701L4 Lenti ORF clone of Tpsb2 (mGFP-tagged) - Mouse tryptase beta 2 (Tpsb2) 10 ug
$600.00

Plasmid Map for MR203701

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203701
Circular map for MR203701

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