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Cops7a (NM_012003) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203690
Cops7a (Myc-DDK-tagged) - Mouse COP9 (constitutive photomorphogenic) homolog, subunit 7a (Arabidopsis thaliana) (Cops7a), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Cops7a or NM_012003"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cops7a
Synonyms D6Ertd35e; SGN7a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203690 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGCGGAGGTGAAGGTGACAGGGCAGAACCAAGAGCAGTTTCTGCTCCTTGCCAAGTCGGCTAAGG
GGGCGGCACTGGCCACACTCATCCACCAGGTGCTGGAGGCCCCTGGTGTCTACGTGTTTGGGGAACTGCT
GGATATGCCTAATGTTAGAGAGCTGGCAGAAAGCGACTTTGCCTCCACCTTCCGGCTGCTCACAGTGTTT
GCCTATGGGACCTATGCGGACTACTTAGCTGAAGCCAGGAATCTCCCCCCACTGACTGACGCACAGAAGA
ATAAGCTTCGACATCTGTCAGTTGTCACTCTGGCTGCCAAAGTCAAGTGTATCCCATATGCAGTGTTGCT
GGAGGCCCTTGCCCTTCGAAACGTGCGCCAGCTGGAAGACCTTGTGATCGAGGCTGTGTATGCTGATGTC
CTTCGTGGCTCTCTGGACCAGCGCAATCAGCGGCTAGAGGTTGATTACAGCATCGGGCGGGACATCCAGC
GCCAGGACCTCAGTGCCATCGCCCAGACCCTGCAAGAGTGGTGCGTGGGCTGTGAGGTTGTGTTGTCGGG
CATCGAAGAGCAGGTCAGCCGTGCCAACCAGCACAAGGAGCAGCAGCTGGGCCTGAAGCAGCAGATCGAA
AGTGAGGTTGCCAACCTTAAGAAAACCATTAAAGTTACGACAGCAGCTGCTGCTGCAGCCACCTCCCAGG
ATCCTGAGCAACACCTGACAGAGCTGAGAGAACCAGCTTCTGGCACCAACCAGCGCCAGCCCAGCAAGAA
AGCCTCCAAGGGCAAGGGACTCCGAGGGAGCGCCAAGATTTGGTCCAAGTCGAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203690 protein sequence
Red=Cloning site Green=Tags(s)

MSAEVKVTGQNQEQFLLLAKSAKGAALATLIHQVLEAPGVYVFGELLDMPNVRELAESDFASTFRLLTVF
AYGTYADYLAEARNLPPLTDAQKNKLRHLSVVTLAAKVKCIPYAVLLEALALRNVRQLEDLVIEAVYADV
LRGSLDQRNQRLEVDYSIGRDIQRQDLSAIAQTLQEWCVGCEVVLSGIEEQVSRANQHKEQQLGLKQQIE
SEVANLKKTIKVTTAAAAAATSQDPEQHLTELREPASGTNQRQPSKKASKGKGLRGSAKIWSKSN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012003
ORF Size 825 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012003.4
RefSeq Size 1775 bp
RefSeq ORF 828 bp
Locus ID 26894
UniProt ID Q9CZ04
Cytogenetics 6 59.17 cM
MW 30.2 kDa
Summary Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively (By similarity).UniProtKB/Swiss-Prot Function
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"NM_012003" in other vectors (4)
SKU Description Size Price
MC200375 Cops7a (untagged) - Mouse COP9 (constitutive photomorphogenic) homolog, subunit 7a (Arabidopsis thaliana) (Cops7a), transcript variant 1, (10ug) 10 ug
$300.00
MG203690 Cops7a (tGFP-tagged) - Mouse COP9 (constitutive photomorphogenic) homolog, subunit 7a (Arabidopsis thaliana) (Cops7a) 10 ug
$500.00
MR203690L3 Lenti ORF clone of Cops7a (Myc-DDK-tagged) - Mouse COP9 (constitutive photomorphogenic) homolog, subunit 7a (Arabidopsis thaliana) (Cops7a), transcript variant 1 10 ug
$600.00
MR203690L4 Lenti ORF clone of Cops7a (mGFP-tagged) - Mouse COP9 (constitutive photomorphogenic) homolog, subunit 7a (Arabidopsis thaliana) (Cops7a), transcript variant 1 10 ug
$600.00

Plasmid Map for MR203690

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203690
Circular map for MR203690

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