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Ccdc3 (NM_028804) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203657
Ccdc3 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 3 (Ccdc3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ccdc3
Synonyms 2310045O21Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203657 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCTCCCGCTGCTGCTCGCCGCGCTCTGCCTCGCCGCCTCCCCGGCGCCCGCGCGCGCCTGCCAGC
TGCCGTCGGAGTGGAGACCCTTGAGCGAAGGCTGCCGCGCCGAGCTAGCCGAGACCATCGTGTATGCCAA
GGTGCTGGCGCTGCACCCCGAGGTGCCTGGCCTCTACAACTACCTGCCGTGGCAGTACCAAGCTGGAGAG
GGAGGGCTCTTCTACTCCGCCGAGGTGGAGATGCTGTGTGACCAGGCGTGGGGCAGTATGTTGGAGGTGC
CCGCCGGCTCCCGGCTCAACCTTACTGGTCTGGGCTACTTCTCCTGCCACTCCCACACGGTGGTCCAGGA
CTACTCTTATTTCTTCTTTGTAAGGATGGATGAGAATTACAATCTCTTGCCACACGGAGTCAATTTCCAA
GATGCCATCTTTCCAGACACCCAGGAGAACAGAAGGATGTTTTCCAGCCTTTTCCAGTTTGCTAACTGTT
CCCAAGGGCAGCAGCTGACGACTTTCTCGAGTGACTGGGAGGTCCAGGAAGACAACAGGCTCATGTGCTC
CTCGGTGCAGAAGGCCCTGTTTGAGGAAGAGGACCATGTCAAGAAGCTGCAGCAGAAGGTGGCCACCCTG
GAGAAGCGGAACAGGCAGCTCCGAGAGCGGGTCAAGAAGGTCAAGAGGTCTCTGAGGCAGGCACGGAAGA
ACAGCCGCCACCTGGAGCTGGTGAACCAAAAACTCAATGAGAAGCTAGGGGCCTCCAGTGCTCAGCAGCA
CATTAACGCTCTGGGCCGGGAGCCCGTGCGTGCCCCCTATCTGCATGGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203657 protein sequence
Red=Cloning site Green=Tags(s)

MPLPLLLAALCLAASPAPARACQLPSEWRPLSEGCRAELAETIVYAKVLALHPEVPGLYNYLPWQYQAGE
GGLFYSAEVEMLCDQAWGSMLEVPAGSRLNLTGLGYFSCHSHTVVQDYSYFFFVRMDENYNLLPHGVNFQ
DAIFPDTQENRRMFSSLFQFANCSQGQQLTTFSSDWEVQEDNRLMCSSVQKALFEEEDHVKKLQQKVATL
EKRNRQLRERVKKVKRSLRQARKNSRHLELVNQKLNEKLGASSAQQHINALGREPVRAPYLHG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028804
ORF Size 819 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028804.1, NP_083080.1
RefSeq Size 2769 bp
RefSeq ORF 822 bp
Locus ID 74186
UniProt ID Q9D6Y1
Cytogenetics 2 A1
MW 31.1 kDa
Summary Negatively regulates TNF-alpha-induced pro-inflammatory response in endothelial cells (ECs) via inhibition of TNF-alpha-induced NF-kappaB activation in ECs (By similarity). Positively regulates lipid accumulation in adipose cells (PubMed:25605713).UniProtKB/Swiss-Prot Function
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"NM_028804" in other vectors (4)
SKU Description Size Price
MC203416 Ccdc3 (untagged) - Mouse coiled-coil domain containing 3 (Ccdc3), (10ug) 10 ug
$300.00
MG203657 Ccdc3 (tGFP-tagged) - Mouse coiled-coil domain containing 3 (Ccdc3) 10 ug
$500.00
MR203657L3 Lenti ORF clone of Ccdc3 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 3 (Ccdc3) 10 ug
$600.00
MR203657L4 Lenti ORF clone of Ccdc3 (mGFP-tagged) - Mouse coiled-coil domain containing 3 (Ccdc3) 10 ug
$600.00

Plasmid Map for MR203657

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR203657
Circular map for MR203657

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