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6720463M24Rik (BC052183) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203649
6720463M24Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 6720463M24 gene (cDNA clone MGC:59525 IMAGE:6336038)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "6720463M24Rik or BC052183"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 6720463M24Rik
Synonyms 6720463M24Rik; AI317232
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203649 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGATGTCAGCGAATTAAAAATGCAAATAACACCAGAAACTCCAGGAAGGATCCCTGTTTTAAATC
CCTTTGAAAGTCCCAGTGATTATTCTAATCTCCATGAACAAACCCTTGCTAGTCCTTCTATCTTTAAGTC
CACAAAACTACCAACTCCAGGGAAATTTAGGTGGTCTATTGATCAGCTGGCCATAATAAACCCTGTAGAG
ATCGATCCAGAGGAGATCCATCGGCAGGCTTCCTACCTAAGGCTTTCTCGAATAGATAAAGATGTGGAAG
ACAAAAGACAAAAAGCCATTGAAGAGTTTTTTACTAAAGATGTCATTGTGCCCTCTCCCTGGACTGATCA
TGATGGAAAACAGCCTTCAGAGCTTCATCCCAGTAAATGCCTAAGTAGCCATGATGACTCTCCTGATGGG
AAAAAGCCAAGTCTTCCCTCACAGAAGTGCAACGCTGCCTGCCAGACTCTGCTGTCTCTTCCTGTGGATT
TTAATTTGGAAGCCATATTAGGAGACTATTTTAGAGAAGAGGACTTTGTGGCCCACACTCCTGGGAACCT
CAGTTCTTCATCTCTGAGAAGAAAGCTCTTTTTGGATGGGAATGGAAGCATCTGTGACCCTTTGCCTTCG
CCGTCGCCCGGAAGTCCTCCCTGCAGTGCCCGGGGGTCGCTCGAGGGACAGTTTTCTTCCAGCCCCATTC
AGAACAGTGTGAAAAAATACAGCTTGGGAAGCGTCACCACGAGCCCGTCGGCCATCTCCTCACCTACCTT
CTCACCTATCGCACTTCAAGGAGGAAAGACGCCGCTCTCAGGTCAAATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203649 protein sequence
Red=Cloning site Green=Tags(s)

MGDVSELKMQITPETPGRIPVLNPFESPSDYSNLHEQTLASPSIFKSTKLPTPGKFRWSIDQLAIINPVE
IDPEEIHRQASYLRLSRIDKDVEDKRQKAIEEFFTKDVIVPSPWTDHDGKQPSELHPSKCLSSHDDSPDG
KKPSLPSQKCNAACQTLLSLPVDFNLEAILGDYFREEDFVAHTPGNLSSSSLRRKLFLDGNGSICDPLPS
PSPGSPPCSARGSLEGQFSSSPIQNSVKKYSLGSVTTSPSAISSPTFSPIALQGGKTPLSGQI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC052183
ORF Size 819 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC052183, AAH52183
RefSeq Size 3866 bp
RefSeq ORF 821 bp
Locus ID 77744
Cytogenetics 14 E2.2
MW 29.7 kDa
Summary Required for the activation of AURKA at the onset of mitosis.UniProtKB/Swiss-Prot Function
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"BC052183" in other vectors (4)
SKU Description Size Price
MC218493 6720463M24Rik (untagged) - Mouse RIKEN cDNA 6720463M24 gene (cDNA clone MGC:59525 IMAGE:6336038), (10ug) 10 ug
$330.00
MG203649 6720463M24Rik (tGFP-tagged) - Mouse RIKEN cDNA 6720463M24 gene (cDNA clone MGC:59525 IMAGE:6336038) 10 ug
$500.00
MR203649L3 Lenti ORF clone of 6720463M24Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 6720463M24 gene (cDNA clone MGC:59525 IMAGE:6336038) 10 ug
$600.00
MR203649L4 Lenti ORF clone of 6720463M24Rik (mGFP-tagged) - Mouse RIKEN cDNA 6720463M24 gene (cDNA clone MGC:59525 IMAGE:6336038) 10 ug
$600.00

Plasmid Map for MR203649

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203649
Circular map for MR203649

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