Srprb (NM_009275) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR203576
Srprb (Myc-DDK-tagged) - Mouse signal recognition particle receptor, B subunit (Srprb)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Srprb
Synonyms AA409543
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203576 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCGGCGAACACCCGGCGAGTTGGCGATGGCGCCGGTGGCGCCTTCCAGCCTTACCTCGACTCCT
TACGACAGGAGCTGCAGCAGAGGGATCCGACGCTGCTCTCCGTGGCGGTGGCTCTCCTGGCGGTGCTGCT
GACGCTGGTGTTCTGGAAGTTCATCTGGAGCCGAAAGAGCAGTCAGAGAGCTGTTCTCTTCGTTGGTCTC
TGTGACTCTGGGAAAACGTTGCTGTTTGTCAGGTTGCTAACTGGCCAGTACAGAGACACACAGACTTCCA
TTACCGATAGTTCTGCCATATACAAAGTCAACAATAACAGGGGTAACAGCCTGACTCTGATCGACCTCCC
CGGGCATGAGAGCTTGAGGTTTCAGCTCTTAGATCGCTTTAAGTCTTCAGCTAGGGCTGTGGTGTTTGTG
GTGGACAGTGCGGCGTTCCAGCGGGAGGTGAAAGATGTGGCCGAATTTCTGTACCAGGTCCTCATCGACA
GCATGGCTCTGAAGAACTCACCGTCCTTATTAATAGCCTGCAATAAGCAAGATATCGCAATGGCAAAATC
AGCGAAGTTGATCCAGCAGCAGCTTGAGAAGGAGCTCAACACCTTACGAGTCACCCGGTCCGCGGCTCCC
AGCACACTGGACAGTTCCAGCACCGCACCTGCTCAGTTGGGGAAGAAAGGCAAAGAATTTGAATTTTCCC
AGTTGCCACTCAAAGTGGAGTTCCTGGAGTGTAGTGCCAAGGGTGGACGAGGGGACACTGGCTCTGCTGA
CATCCAGGACTTGGAGAAGTGGCTGGCCAAGATTGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR203576 protein sequence
Red=Cloning site Green=Tags(s)

MASANTRRVGDGAGGAFQPYLDSLRQELQQRDPTLLSVAVALLAVLLTLVFWKFIWSRKSSQRAVLFVGL
CDSGKTLLFVRLLTGQYRDTQTSITDSSAIYKVNNNRGNSLTLIDLPGHESLRFQLLDRFKSSARAVVFV
VDSAAFQREVKDVAEFLYQVLIDSMALKNSPSLLIACNKQDIAMAKSAKLIQQQLEKELNTLRVTRSAAP
STLDSSSTAPAQLGKKGKEFEFSQLPLKVEFLECSAKGGRGDTGSADIQDLEKWLAKIA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009275
ORF Size 807 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009275.4, NP_033301.1
RefSeq Size 2991 bp
RefSeq ORF 810 bp
Locus ID 20818
UniProt ID P47758
Cytogenetics 9 F1
MW 29.6 kDa
Summary Component of the SRP (signal recognition particle) receptor. Ensures, in conjunction with the signal recognition particle, the correct targeting of the nascent secretory proteins to the endoplasmic reticulum membrane system. Has GTPase activity. May mediate the membrane association of SRPR.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009275" in other vectors (4)
SKU Description Size Price
MC207408 Srprb (untagged) - Mouse signal recognition particle receptor, B subunit (Srprb), (10ug) 10 ug
$330.00
MG203576 Srprb (tGFP-tagged) - Mouse signal recognition particle receptor, B subunit (Srprb) 10 ug
$500.00
MR203576L3 Lenti ORF clone of Srprb (Myc-DDK-tagged) - Mouse signal recognition particle receptor, B subunit (Srprb) 10 ug
$600.00
MR203576L4 Lenti ORF clone of Srprb (mGFP-tagged) - Mouse signal recognition particle receptor, B subunit (Srprb) 10 ug
$600.00

Plasmid Map for MR203576

Download sequence for

Cloning scheme for MR203576

Circular map for MR203576

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