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Eaf1 (NM_028932) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203554
Eaf1 (Myc-DDK-tagged) - Mouse ELL associated factor 1 (Eaf1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Eaf1
Synonyms 4933403C17Rik; AI666536
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203554 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACGGCACGGCGAACCCGCTGCTGGACCGGGAGGAACACTGTCTGCGGCTAGGGGAAAGCTTCGAGA
AGCGGCCGCGGGCCTCCTTCCACACGATTCGCTACGATTTTAAGCCAGCATCAATAGACACTTCCTGTGA
AGGAGAACTACAGGTTGGCAAAGGAGATGAAGTCACCATTACGCTGCCACACATCCCTGGGTCCACGCCG
CCAATGACGGTGTTCAAAGGGAACAAGCGGCCCTATCAGAAAGACTGTGTGCTCATTATTAACCACGACA
CTGGAGAGTATGTGCTGGAGAAGCTGAGCAGCAGCATTCAGGTCAAGAAGACAAGAGCTGAGGGAAGCAG
TAAAATCCAAGCTCGAATGGAGCAGCAGCCCGCTCGTCCCCCACAGCCGTCACAGCCACCCCCTCCACCT
CCACCCATGCCGTTCCGAGCCCCAACGAAGCCTCCAGCTGGACCCAAAACTTCTCCCCTGAAGGATAACC
CCTCACCTGAGCCCCAGTTGGATGACATCAAAAGAGAGCTGAGGGCCGAGGTGGACATTATTGAGCAGAT
GAGCAGCAGCAGTGGGAGCAGCTCCTCCGACTCTGAGAGCTCCTCGGGAAGTGACGACGACAGCTCCAGC
AGCGCAGGCGAAGACAATGGCCCAGCCTCGCCTCCCCAGCCCTCACACCAGCAGCCCTACAACAGCAGGC
CTGCGGTGGCCAATGGAACCAGCCGGCCCCAAGGAAGCTCTCAGCTCATGAACACGCTCAGAAATGACCT
GCAGCTAAGTGAGTCTGGCAGTGACAGTGATGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203554 protein sequence
Red=Cloning site Green=Tags(s)

MNGTANPLLDREEHCLRLGESFEKRPRASFHTIRYDFKPASIDTSCEGELQVGKGDEVTITLPHIPGSTP
PMTVFKGNKRPYQKDCVLIINHDTGEYVLEKLSSSIQVKKTRAEGSSKIQARMEQQPARPPQPSQPPPPP
PPMPFRAPTKPPAGPKTSPLKDNPSPEPQLDDIKRELRAEVDIIEQMSSSSGSSSSDSESSSGSDDDSSS
SAGEDNGPASPPQPSHQQPYNSRPAVANGTSRPQGSSQLMNTLRNDLQLSESGSDSDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028932
ORF Size 804 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028932.1, NM_028932.2, NM_028932.3, NM_028932.4, NP_083208.1
RefSeq Size 4238 bp
RefSeq ORF 807 bp
Locus ID 74427
UniProt ID Q9D4C5
Cytogenetics 14 B
MW 29 kDa
Summary Acts as a transcriptional transactivator of ELL and ELL2 elongation activities.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028932" in other vectors (4)
SKU Description Size Price
MC207743 Eaf1 (untagged) - Mouse ELL associated factor 1 (Eaf1), (10ug) 10 ug
$330.00
MG203554 Eaf1 (tGFP-tagged) - Mouse ELL associated factor 1 (Eaf1) 10 ug
$500.00
MR203554L3 Lenti ORF clone of Eaf1 (Myc-DDK-tagged) - Mouse ELL associated factor 1 (Eaf1) 10 ug
$600.00
MR203554L4 Lenti ORF clone of Eaf1 (mGFP-tagged) - Mouse ELL associated factor 1 (Eaf1) 10 ug
$600.00

Plasmid Map for MR203554

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203554
Circular map for MR203554

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