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Gpm6a (BC023461) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203545
Gpm6a (Myc-DDK-tagged) - Mouse glycoprotein m6a (cDNA clone MGC:32434 IMAGE:5041793)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gpm6a
Synonyms MGC38999, M6A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203545 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGTGCTTCGAGTGCTGCATTAAATGCCTGGGAGGTATTCCCTATGCTTCTCTGATTGCAACCATCC
TGCTGTATGCAGGCGTTGCCCTGTTCTGTGGCTGTGGCCATGAAGCCCTTTCTGGAACAGTCAACATTCT
GCAGACCTACTTTGAGTTGGCAAGGACTGCTGGAGACACACTGGATGTTTTCACTATGATTGACATCTTT
AAGTATGTGATCTATGGCATTGCGGCTGCTTTCTTTGTCTATGGCATTTTACTGATGGTAGAAGGTTTCT
TCACAACTGGGGCTATCAAAGATCTCTATGGAGACTTCAAAATCACCACCTGTGGCAGATGTGTGAGCGC
TTGGTTTATCATGCTGACATACCTCTTCATGTTGGCCTGGCTGGGAGTCACAGCTTTCACCTCACTGCCC
GTGTACATGTATTTCAATGTGTGGACCATCTGCCGGAACACCACTCTAGTGGAGGGAGCAAATCTCTGCT
TGGATCTGCGTCAGTTTGGGATTGTGACAATTGGAGAGGAAAAGAAAATTTGTACTGCCTCTGAGAACTT
CCTGAGGATGTGTGAATCTACTGAGCTGAATATGACCTTCCACTTGTTCATTGTGGCACTTGCTGGAGCT
GGAGCAGCAGTTATTGCTATGGTCCACTACCTGATGGTTCTGTCTGCCAACTGGGCCTATGTGAAAGATG
CCTGCCGCATGCAGAAGTACGAAGACATCAAGTCAAAGGAAGAGCAGGAGCTGCACGACATCCATTCTAC
TCGCTCTAAAGAGCGGCTCAATGCGTACACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203545 protein sequence
Red=Cloning site Green=Tags(s)

MGCFECCIKCLGGIPYASLIATILLYAGVALFCGCGHEALSGTVNILQTYFELARTAGDTLDVFTMIDIF
KYVIYGIAAAFFVYGILLMVEGFFTTGAIKDLYGDFKITTCGRCVSAWFIMLTYLFMLAWLGVTAFTSLP
VYMYFNVWTICRNTTLVEGANLCLDLRQFGIVTIGEEKKICTASENFLRMCESTELNMTFHLFIVALAGA
GAAVIAMVHYLMVLSANWAYVKDACRMQKYEDIKSKEEQELHDIHSTRSKERLNAYT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC023461
ORF Size 801 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC023461, AAH23461
RefSeq Size 2780 bp
RefSeq ORF 803 bp
Locus ID 234267
Cytogenetics 8 B1.3
MW 29.8 kDa
Summary Involved in neuronal differentiation, including differentiation and migration of neuronal stem cells. Plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation. Gpm6a-induced filopodia formation involves mitogen-activated protein kinase (MAPK) and Src signaling pathways. Conflictingly, PubMed:22162747 reports that induced cellular protrusions are simple membrane-wrapped tubules without actin or tubulin-based cytoskeletons and with Gpm6a gliding along membrane edges indicative for a function in actin-independent membrane deformation. May be involved in neuronal NGF-dependent Ca(2+) influx. May be involved in regulation of endocytosis and intracellular trafficking of G-protein-coupled receptors (GPCRs); enhances internalization and recycling of mu-type opioid receptor.UniProtKB/Swiss-Prot Function
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"BC023461" in other vectors (3)
SKU Description Size Price
MG203545 Gpm6a (tGFP-tagged) - Mouse glycoprotein m6a (cDNA clone MGC:32434 IMAGE:5041793) 10 ug
$500.00
MR203545L3 Lenti ORF clone of Gpm6a (Myc-DDK-tagged) - Mouse glycoprotein m6a (cDNA clone MGC:32434 IMAGE:5041793) 10 ug
$600.00
MR203545L4 Lenti ORF clone of Gpm6a (mGFP-tagged) - Mouse glycoprotein m6a (cDNA clone MGC:32434 IMAGE:5041793) 10 ug
$600.00

Plasmid Map for MR203545

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203545
Circular map for MR203545

file-alt Citations

A mammalian nervous-system-specific plasma membrane proteasome complex that modulates neuronal function
Ramachandran, Margolis
Nat. Struct. Mol. Biol. (2017)
AI Summary: OriGene products, specifically the full-length mouse tagged GPM6A, tagged GPM6B, and tagged β5 constructs, were used to investigate the components and function of the nervous-system-specific membrane proteasome complex described in the study.
Snippet: The full-length mouse tagged GPM6A, tagged GPM6B, and tagged β 5 constructs were acquired from Origene
Showing 1-1 of 1 papers.
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