Tprgl (NM_026388) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR203533
Tprgl (Myc-DDK-tagged) - Mouse transformation related protein 63 regulated like (Tprgl)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tprgl
Synonyms 1200015A19Rik; mover; Svap30; Tprg1l
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203533 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCAGCTGCGGGACACGGTGGACTCGGCGGGCACGAGCCCCACGGCGGTGCTGGCGGCCGGCGAGG
ACGCGGGTGCGGGCCGGCCTGGGGCGGGCACACCGCTGCGCCAGACGTTGTGGCCGCTGAACGTCCACGA
CCCTACACGCCGCGCCCGCGTCAAGGAGTACTTCGTGTTCCGGCCCGGCACCATCGAGCAGGCGGTCGAG
GAGATCCGCGCCGTGGTACGGCCCGTGGAGGACGGCGAGATCCAGGGGGTGTGGCTGCTGACCGAGGTGG
ACCACTGGAACAACGAGAAGGAGCGGCTGGTGCTGGTCACCGACCAGTCGCTGCTCATCTGCAAATATGA
CTTCATCAGCCTTCAGTGCCAGCAGGTGGTCAGGGTGGCGCTCAGCGCGGTAGACACCATCTCCTGTGGA
GAATTCCAGTTTCCTCCGAAGTCGCTCAACAAGAGAGAAGGCTTTGGGGTTCGCATTCAGTGGGACAAGC
AGAGCCGCCCTTCCTTCATCAACAGATGGAACCCCTGGTCTACAAACATGCCCTATGCCACCTTCATAGA
ACACCCAATGGCTGGCATGGACGAGAAGACAGCGTCTCTGTGCCATTTGGAAAGCTTCAAGGCTCTCCTG
ATCCAAGCTGTCAAGAAGGCCCAGAAGGAGAGCCCACTGCCAGGGCAGGCGAATACCGTGCTGGTGCTGG
AGCGCCCCCTCCTCATCGAGACCTACGTGGGGCTCATGTCCTTCATCAACAATGAAGCCAAGCTGGGCTA
CTCCATGACCAGGGGCAAGATAGGCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR203533 protein sequence
Red=Cloning site Green=Tags(s)

MLQLRDTVDSAGTSPTAVLAAGEDAGAGRPGAGTPLRQTLWPLNVHDPTRRARVKEYFVFRPGTIEQAVE
EIRAVVRPVEDGEIQGVWLLTEVDHWNNEKERLVLVTDQSLLICKYDFISLQCQQVVRVALSAVDTISCG
EFQFPPKSLNKREGFGVRIQWDKQSRPSFINRWNPWSTNMPYATFIEHPMAGMDEKTASLCHLESFKALL
IQAVKKAQKESPLPGQANTVLVLERPLLIETYVGLMSFINNEAKLGYSMTRGKIGF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026388
ORF Size 798 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026388.2, NP_080664.1
RefSeq Size 1756 bp
RefSeq ORF 801 bp
Locus ID 67808
UniProt ID Q9DBS2
Cytogenetics 4 E2
MW 29.8 kDa
Summary Presynaptic protein involved in the synaptic transmission tuning. Regulates synaptic release probability by decreasing the calcium sensitivity of release.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026388" in other vectors (4)
SKU Description Size Price
MC201234 Tprgl (untagged) - Mouse transformation related protein 63 regulated like (Tprgl), (10ug) 10 ug
$300.00
MG203533 Tprgl (tGFP-tagged) - Mouse RIKEN cDNA 1200015A19 gene (1200015A19Rik) 10 ug
$500.00
MR203533L3 Lenti ORF clone of Tprgl (Myc-DDK-tagged) - Mouse transformation related protein 63 regulated like (Tprgl) 10 ug
$600.00
MR203533L4 Lenti ORF clone of Tprgl (mGFP-tagged) - Mouse transformation related protein 63 regulated like (Tprgl) 10 ug
$600.00

Plasmid Map for MR203533

Download sequence for

Cloning scheme for MR203533

Circular map for MR203533

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