Cdca3 (NM_013538) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR203531
Cdca3 (Myc-DDK-tagged) - Mouse cell division cycle associated 3 (Cdca3)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cdca3
Synonyms 2410005A12Rik; C8; Grcc8; Tome-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203531 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTTCGACTCAGAGCGTCTCAGGCACTCCAGCACGGCCTCTGCCACGCAACAAGCAAGTGGCTCGAG
TAGCAGACCCTCGTTCACCTAGTGCTGGCATCCAGCGCACTCCTATTCAGGTAGAGAGCTCTCCACAGCC
AAGCCTACCAGCAGAACAGCTGAACGGTCTCAAACAGGCACAAGACCCAGATCCCCGCTCTCCTACTCTT
GGCATTGCACGGACACCCATGAAGATCAGTGGTCCAGACCCTCAGTGCTCACTGGTGAAAGAGCTGAGCG
AAGTATTGGAGACGGAAGCGTCGGAATCGATTTCCTCCCCAGAGCTTGCTCTGCCCCGGGAAACGCCTTT
ATTTTCTGACCTGGACCTGTCTTCAGGTCCTCAGTTATCCCCTGAGGACCAGTTACTGCCTTGGAGCCAG
GCTGAACTCGATCCCAAACAGGTGTTTACCAAGGAGGAAGCCAAACAATCCGCAGAAACTATAGCTGCCA
GCCAGAACTCAGACAAGCCCTCCAGAGACCCAGAGACTCCCCAGTCCTCAGGTTCTAAGCGCAGCAGACG
AAAAGCAAACAGCAAGGTTCTAGGGAGGTCCCCTCTCACCATCCTGCAGGATGACAACTCCCCTGGGACC
TTGACACTACGACAGGGTAAGCGGCCTTCTGCCCTCAGTGAGAACGTTAAGGACCTAAAGGAAGGAGTCG
TTCTTGGAACTGGAAGATTTCTCAAAGCTGGAGGAGGAGCACGGGAGCCAAACCAGGACCACGACAAGGA
AAATCAGCATTTTGCCTTGTTGGAGAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR203531 protein sequence
Red=Cloning site Green=Tags(s)

MGSTQSVSGTPARPLPRNKQVARVADPRSPSAGIQRTPIQVESSPQPSLPAEQLNGLKQAQDPDPRSPTL
GIARTPMKISGPDPQCSLVKELSEVLETEASESISSPELALPRETPLFSDLDLSSGPQLSPEDQLLPWSQ
AELDPKQVFTKEEAKQSAETIAASQNSDKPSRDPETPQSSGSKRSRRKANSKVLGRSPLTILQDDNSPGT
LTLRQGKRPSALSENVKDLKEGVVLGTGRFLKAGGGAREPNQDHDKENQHFALLES

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013538
ORF Size 798 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013538.1, NM_013538.2, NM_013538.3, NM_013538.4, NM_013538.5, NP_038566.1
RefSeq Size 1488 bp
RefSeq ORF 801 bp
Locus ID 14793
UniProt ID Q99M54
Cytogenetics 6 59.17 cM
MW 28.6 kDa
Summary F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013538" in other vectors (4)
SKU Description Size Price
MC200366 Cdca3 (untagged) - Mouse cell division cycle associated 3 (Cdca3), (10ug) 10 ug
$300.00
MG203531 Cdca3 (tGFP-tagged) - Mouse cell division cycle associated 3 (Cdca3) 10 ug
$500.00
MR203531L3 Lenti ORF clone of Cdca3 (Myc-DDK-tagged) - Mouse cell division cycle associated 3 (Cdca3) 10 ug
$600.00
MR203531L4 Lenti ORF clone of Cdca3 (mGFP-tagged) - Mouse cell division cycle associated 3 (Cdca3) 10 ug
$600.00

Plasmid Map for MR203531

Download sequence for

Cloning scheme for MR203531

Circular map for MR203531

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