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Apool (NM_026565) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203508
Apool (Myc-DDK-tagged) - Mouse apolipoprotein O-like (Apool)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Apool
Synonyms 6720473G16Rik; 9430083G14Rik; E130106L15Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203508 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCTTTAGGATGGGAAAGTTGACAACCATACCTGCAGGTCTGATATATGCATCTATAAATGTGC
GTCTTGCCAAAGAAGAGGAACCCAAAAAGCAGCTAGTGAGACCGGACCAGCTCCCCATATATACTGCACC
ACCCCTCCATTCTAAATATGTTGAAGAACAGCCTGGTAATTTACAAAGGGGCTTTGCATCCATCCGTACT
ACAACCGTGTATTATATTGGCTGGTGCAAGAGCATTTATCTCTTTATGAAGAATGGGGTTATGGATACAG
TACAGTTTGGAAAAGATGCATATGTCTATCTGAAGAATCCTCCGCAAGATTTTCTCCCCAAAATGGGAGT
GATTACAGCTTCGGGACTGGCAGGTTTGCTTTCAGCAAGAAAAGGTTCCAGGTTTAAGAAGATTGCTTAT
CCACTAGGACTGGCCACATTAGGAGCGACTGTTTGCTACCCAGCTCAGTCAGTAATAATTGCAAAGATAA
CTGGGAAAAAGGCATATGCTACAAGCCAGCAAATTTTTCAAGCCATTAAGTCATTGTGGACACAAAAAAG
TGAAAACGAATCACTCCCTGAACCAAAGGAAGAAAGCAAGGAAGGAAGGTCTGATGAAATCCATGCCAGC
TTGCCTGACCTGAAACATTCAGTGTCTTTGCCAAAAGAACTTGCCTCTGCAACAGTGATTAAATCAGAAT
CTACCTCAGGTACTACACAGTTCATTCCTGACCCCAAGCTCATGGATCATGGGCAGTCTCATCCAGATGA
TAAAGATATGTACAGCACTAGAAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203508 protein sequence
Red=Cloning site Green=Tags(s)

MAAFRMGKLTTIPAGLIYASINVRLAKEEEPKKQLVRPDQLPIYTAPPLHSKYVEEQPGNLQRGFASIRT
TTVYYIGWCKSIYLFMKNGVMDTVQFGKDAYVYLKNPPQDFLPKMGVITASGLAGLLSARKGSRFKKIAY
PLGLATLGATVCYPAQSVIIAKITGKKAYATSQQIFQAIKSLWTQKSENESLPEPKEESKEGRSDEIHAS
LPDLKHSVSLPKELASATVIKSESTSGTTQFIPDPKLMDHGQSHPDDKDMYSTRS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026565
ORF Size 795 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026565.2, NP_080841.1
RefSeq Size 1001 bp
RefSeq ORF 798 bp
Locus ID 68117
UniProt ID Q78IK4
Cytogenetics X E1
MW 29.3 kDa
Summary Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Specifically binds to cardiolipin (in vitro) but not to the precursor lipid phosphatidylglycerol. Plays a crucial role in crista junction formation and mitochondrial function.UniProtKB/Swiss-Prot Function
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"NM_026565" in other vectors (4)
SKU Description Size Price
MC201850 Apool (untagged) - Mouse apolipoprotein O-like (Apool), (10ug) 10 ug
$300.00
MG203508 Apool (tGFP-tagged) - Mouse apolipoprotein O-like (Apool) 10 ug
$500.00
MR203508L3 Lenti ORF clone of Apool (Myc-DDK-tagged) - Mouse apolipoprotein O-like (Apool) 10 ug
$600.00
MR203508L4 Lenti ORF clone of Apool (mGFP-tagged) - Mouse apolipoprotein O-like (Apool) 10 ug
$600.00

Plasmid Map for MR203508

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203508
Circular map for MR203508

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