Cdca5 (NM_026410) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR203497
Cdca5 (Myc-DDK-tagged) - Mouse cell division cycle associated 5 (Cdca5)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cdca5
Synonyms 2610036L13Rik; AL024086; AW536684; C85404
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203497 representing NM_026410
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGCGGCGAACACGATCAGGAGGGGCTGCCCAGCGGTCTGGGCCCAGGACTTCACTTACTAAGC
CTTCGAAGTCGTCCAAGCGGAAATCTGGCTCAGATCTCCCGAACTCGTTCTCTGAAATCTGGCCGAGGAC
AACTCCAGCGGTCCCAGTCAGAAAGGCCATCGTCTTGAAGAAGATCGTAGCTCATGCAGTAGAGGTCCCA
GATGTCCACACGGTTCGAAGAAGTCCTAGGATCTCTTTTATTTTGGAAAAAGAGAACAACCCTCCGCTCA
AGGTACCCACGAAAGAAGACCTCTTCAAGACTTGTAGTGTCCCTGGTACTCCCTCCAGCACCCCCGTGCT
GTACACTCAGAATGTTGAGCCTGACTCTGGAGAAGCAGAGCTGGACAGCAGAGACCTGGAAATGTCTCAG
AAAGTCAGGCGGTCGTACAGTCGGCTACAGAGCCTTGGTTGTGCCTCAACCTCCACCCCTGGCCGCCGAT
CCTTCTTTGGCTTTGAGGGACCTGACGACTTGCCTGGAGTCTCACCTGTCGTGTGTTCAAAGTTAATCGA
GACCCCAAAGGTACCTGCAAAGGACTTGGTTCCGGCTAGAACGAAGGACTTGGTTCCAGATAGTACGAAG
GACTTGGTTCCGGCTAGAACGCTCCCTGGAATCTCTCCCCCAGTTGTGAAAGAGAAACGAAAGAAGAAGG
TTCCTGAAATCCTGAAATCAGAGCTGGATAAGTGGGCTGTGGCCATGAATGCTGAATTTGAAGCTGCCGA
GCAGTTTGAGCTTCTGATTGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR203497 representing NM_026410
Red=Cloning site Green=Tags(s)

MAERRTRSGGAAQRSGPRTSLTKPSKSSKRKSGSDLPNSFSEIWPRTTPAVPVRKAIVLKKIVAHAVEVP
DVHTVRRSPRISFILEKENNPPLKVPTKEDLFKTCSVPGTPSSTPVLYTQNVEPDSGEAELDSRDLEMSQ
KVRRSYSRLQSLGCASTSTPGRRSFFGFEGPDDLPGVSPVVCSKLIETPKVPAKDLVPARTKDLVPDSTK
DLVPARTLPGISPPVVKEKRKKKVPEILKSELDKWAVAMNAEFEAAEQFELLIE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026410
ORF Size 792 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026410.3, NP_080686.1
RefSeq Size 1906 bp
RefSeq ORF 795 bp
Locus ID 67849
UniProt ID Q9CPY3
Cytogenetics 19 A
MW 29.4 kDa
Summary Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026410" in other vectors (4)
SKU Description Size Price
MC202058 Cdca5 (untagged) - Mouse cell division cycle associated 5 (Cdca5), (10ug) 10 ug
$300.00
MG203497 Cdca5 (tGFP-tagged) - Mouse cell division cycle associated 5 (Cdca5) 10 ug
$500.00
MR203497L3 Lenti ORF clone of Cdca5 (Myc-DDK-tagged) - Mouse cell division cycle associated 5 (Cdca5) 10 ug
$600.00
MR203497L4 Lenti ORF clone of Cdca5 (mGFP-tagged) - Mouse cell division cycle associated 5 (Cdca5) 10 ug
$600.00

Plasmid Map for MR203497

Download sequence for

Cloning scheme for MR203497

Circular map for MR203497

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